中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
8期
606-609
,共4页
口腔肿瘤%血管生成素2%淋巴结转移%肿瘤分期
口腔腫瘤%血管生成素2%淋巴結轉移%腫瘤分期
구강종류%혈관생성소2%림파결전이%종류분기
Oral neoplasms%Angiopoietin-2%Lymph node metastasis%Neoplasm stage
目的 检测血管生成素2(Ang-2)在口腔颌面部鳞癌中的表达,分析其表达强度与淋巴结转移和临床分期等临床生物学行为的关系,并探讨Ang-2在口腔颌面部恶性肿瘤生长中的作用.方法 采用实时荧光定量聚合酶链反应(PCR)法检测23例口腔颌面部鳞癌组织中Ang-2 mRNA的表达;采用免疫组化半定量法检测28例口腔颌面部鳞癌组织中Ang-2蛋白的表达.结果 Ang-2mRNA在口腔颌面部鳞癌组织中表达的dCt值为6.86±1.37,在癌旁无瘤组织中表达的dCt值为7.95±2.08,差异有统计学意义(P＜0.05).Ang-2蛋白阳性染色定位于肿瘤细胞和血管内皮细胞的胞浆和胞膜中,在肿瘤组织及癌旁无瘤组织中的阳性表达率分别为53.6%和24.0%,差异有统计学意义(P＜0.05).Ang-2 mRNA在淋巴结转移阴性和阳性组中表达的dCt值分别为7.16±1.49和6.48±1.16;在不同临床分期组(Ⅰ、Ⅱ期与Ⅲ、Ⅳ期)中表达的dCt值分别为7.11±1.63和6.49±1.10,差异均无统计学意义(均P＞0.05).结论 Ang-2参与了口腔颌面部肿瘤血管的形成.Ang-2mRNA的表达强度与淋巴结转移及临床分期无明显的相关性,有待更大样本量的研究来进一步论证.
目的 檢測血管生成素2(Ang-2)在口腔頜麵部鱗癌中的錶達,分析其錶達彊度與淋巴結轉移和臨床分期等臨床生物學行為的關繫,併探討Ang-2在口腔頜麵部噁性腫瘤生長中的作用.方法 採用實時熒光定量聚閤酶鏈反應(PCR)法檢測23例口腔頜麵部鱗癌組織中Ang-2 mRNA的錶達;採用免疫組化半定量法檢測28例口腔頜麵部鱗癌組織中Ang-2蛋白的錶達.結果 Ang-2mRNA在口腔頜麵部鱗癌組織中錶達的dCt值為6.86±1.37,在癌徬無瘤組織中錶達的dCt值為7.95±2.08,差異有統計學意義(P＜0.05).Ang-2蛋白暘性染色定位于腫瘤細胞和血管內皮細胞的胞漿和胞膜中,在腫瘤組織及癌徬無瘤組織中的暘性錶達率分彆為53.6%和24.0%,差異有統計學意義(P＜0.05).Ang-2 mRNA在淋巴結轉移陰性和暘性組中錶達的dCt值分彆為7.16±1.49和6.48±1.16;在不同臨床分期組(Ⅰ、Ⅱ期與Ⅲ、Ⅳ期)中錶達的dCt值分彆為7.11±1.63和6.49±1.10,差異均無統計學意義(均P＞0.05).結論 Ang-2參與瞭口腔頜麵部腫瘤血管的形成.Ang-2mRNA的錶達彊度與淋巴結轉移及臨床分期無明顯的相關性,有待更大樣本量的研究來進一步論證.
목적 검측혈관생성소2(Ang-2)재구강합면부린암중적표체,분석기표체강도여림파결전이화림상분기등림상생물학행위적관계,병탐토Ang-2재구강합면부악성종류생장중적작용.방법 채용실시형광정량취합매련반응(PCR)법검측23례구강합면부린암조직중Ang-2 mRNA적표체;채용면역조화반정량법검측28례구강합면부린암조직중Ang-2단백적표체.결과 Ang-2mRNA재구강합면부린암조직중표체적dCt치위6.86±1.37,재암방무류조직중표체적dCt치위7.95±2.08,차이유통계학의의(P＜0.05).Ang-2단백양성염색정위우종류세포화혈관내피세포적포장화포막중,재종류조직급암방무류조직중적양성표체솔분별위53.6%화24.0%,차이유통계학의의(P＜0.05).Ang-2 mRNA재림파결전이음성화양성조중표체적dCt치분별위7.16±1.49화6.48±1.16;재불동림상분기조(Ⅰ、Ⅱ기여Ⅲ、Ⅳ기)중표체적dCt치분별위7.11±1.63화6.49±1.10,차이균무통계학의의(균P＞0.05).결론 Ang-2삼여료구강합면부종류혈관적형성.Ang-2mRNA적표체강도여림파결전이급림상분기무명현적상관성,유대경대양본량적연구래진일보론증.
Objective To investigate the expression of angiopaietin-2(Ang-2)and its clinical significance in oral squamons cell carcinoma.Methods The expression of Ang-2 mRNA was measured by real-time RT-PCR,and the expression of Ang-2 protein in tissue samples was detected by immunohistochemical staining.Results The mean dCt value of Ang-2 mRNA expression in the cancer tissue was 6.86±1.37,significantly lower than that in the paired adjacent non-cancerous tissue(7.95±2,08,P＜0.05),indicating a significantly higher expression of Ang-2 mRNA in the cancerous tissue than that in the adjacent non-cancerous tissue.The distribution of Ang-2 protein was found not only in the vascular endothelial ceils but also in tumor cells.Semi-quantitative analysis revealed that the expression of Ang-2 protein in tumor specimens(53.6%)was significantly higher than that(24.0%)in the paired adjacent non-cancerous tissue(P＜0.05),the result was well consistent with that measured by RT-PCR.The dCt value of Ang-2 mRNA expression was 6.48±1.16 in the patients with metastasis in lymph nodes versus 7.16±1.49 in those without,with a non-significant difierence between the two groups(P＞0.05).As regards the clinical stages,no significant difference was found between the expressious of Ang-2 mRNA in stage Ⅰ+Ⅱ(7.11±1.63)and stage Ⅲ+Ⅳ cases(6.49±1.10,P＞0.05).Condusion Angiopoietin-2 protein is expressed not only in vascular endothelial cells,but also in tumor cells,suggesting that angiopoietin-2 may take part in angiogenesis in oral squamons cell carcinoma.However,our results that high expression of angiopoietin-2 mRNA is not correlated with lymph node metastasis and clinical stages.needs to be further verified in a large scale study.
