CAJ | 학술논문

采用RP-HPLC法建立了同时测定珠芽蓼中牡荆素、槲皮苷、槲皮素三种黄酮的方法,色谱柱为kromasil C18(250 mm×4.6mm,5μm),以甲醇-水(含0.25％磷酸)为流动相梯度洗脱,流速1 mL/min,检测波长360 nm.结果表明,牡荆素、槲皮苷和槲皮素质量浓度在8～40、5～50 μg/mL和5.33～52 μg/mL范围内与色谱峰面积均呈良好的线形关系,平均回收率分别为100.3％、98.9％和100.4％,日内稳定性偏差分别为0.78％,0.51％和0.38％,日间稳定性偏差为2.6％,1.9％和2.1％.该方法简单、准确,可为珠芽蓼的质量控制提供科学依据.
채용RP-HPLC법건립료동시측정주아료중모형소、곡피감、곡피소삼충황동적방법,색보주위kromasil C18(250 mm×4.6mm,5μm),이갑순-수(함0.25％린산)위류동상제도세탈,류속1 mL/min,검측파장360 nm.결과표명,모형소、곡피감화곡피소질량농도재8～40、5～50 μg/mL화5.33～52 μg/mL범위내여색보봉면적균정량호적선형관계,평균회수솔분별위100.3％、98.9％화100.4％,일내은정성편차분별위0.78％,0.51％화0.38％,일간은정성편차위2.6％,1.9％화2.1％.해방법간단、준학,가위주아료적질량공제제공과학의거.
To develop a 8ensitive and specific reversed-phase high perfonnance liquid chromatography ( RP-HPLC) method for simultaneous detennination of three flavoniods in Polygonum viviparum,the HPLC separation was performed on a Kromasil C18 column (250 mm ×4.6 mm,5 μm) and detected with DAD at 360 nm.The gradient elution was carried out with methano-H2O (containing 0.25％ phosphoric acid) as the mobile phase at a flow rate of 1 mL/min.The results indicated that the calibration cunre8 for viterxin,quercetin and quercitrin were linear over the ranges of 8-40 μg/mL,5-50 μg/mL and 5.33 -52 μg/mL,their assay recoveries were 100.3％,98.9％and 100.4％,their intraday repeatability were 0.78％,0.51％ and 0.38％,and interday repeatability were 2.6％,1.9％ and 2.1％,respectively.This method was simple,accurate and sensitive,so it could be used for the quality control of P.viviparum.<英文关键词>=Key words:Polygonum viviparum ; viterxin ; quercetin ; quercitrin ; RP-HPLC