CAJ | 학술논문

研究了1种检测酵母菌产酒精能力的新方法。利用酒精发酵液体培养基在厌氧条件下对产酒精酵母菌株YE—1、YE-2、YE-3进行酒精发酵培养，同时利用菌体发酵培养基在有氧条件下进行菌体培养实验作为对照。通过分光光度法检测培养基中葡萄糖的消耗量，根据所消耗的葡萄糖中用于酒精发酵的量，推算出3株酵母菌菌株在酒精发酵培养基中酒精含量分别为4．03mg／mL、3．50mg／mL和3．77mg／mL，进而比较出各菌株产酒精能力的大小。
연구료1충검측효모균산주정능력적신방법。이용주정발효액체배양기재염양조건하대산주정효모균주YE—1、YE-2、YE-3진행주정발효배양，동시이용균체발효배양기재유양조건하진행균체배양실험작위대조。통과분광광도법검측배양기중포도당적소모량，근거소소모적포도당중용우주정발효적량，추산출3주효모균균주재주정발효배양기중주정함량분별위4．03mg／mL、3．50mg／mL화3．77mg／mL，진이비교출각균주산주정능력적대소。
A new method for the evaluation of ethanol-producing capability of microzyme had been developed. In the experiments, while microzyme cells were cultured in cell culture mediums under aerobic conditions, ethanol-producing microzyme strains YE-1, YE-2 and YE-3 were cultured for alcohol fermentation in liquid culture mediums under anaerobic conditions. The amount of glucose consumption in alcohol fermentation liquid mediums was detected by spectrophotometric assay. Then alcohol concentration was calculated according to the amount of glucose consumption for alcohol fermentation. The alcohol concentration in these three alcohol fermentation aleohol mediums were 4.03 mg / mL, 3.50 mg / mL and 3.77 mg / mL respectively, and then ethanol-producing capability of each strain was revealed.