目的 考察黄芩苷在不同pH值中的平衡溶解度与表观油水分配系数,为制剂研究奠定基础.方法 采用摇瓶-紫外分光光度法测定温度为25和37 ℃时,黄芩苷在不同pH值磷酸盐缓冲溶液中的平衡溶解度及在正辛醇/缓冲液体系中的表观油水分配系数.结果 25 ℃时,黄芩苷在pH=2.0,3.0,4.0,5.0,6.0,6.8,7.4,8.0和9.0缓冲液中的平衡溶解度分别为0.032,0.034,0.119,0.873,3.329,12.96,11.49,4.605和11.87 mg·mL-1,相应条件下表观油水分配系数(P)值分别为0.363,0.244,0.292,0.137,0.057,0.046,0.036,0.028和0.029.37 ℃时,黄芩苷在pH=2.0,3.0,4.0,5.0,6.0,6.8,7.4,8.0和9.0缓冲液中的平衡溶解度分别为0.028,0.048,0.095,0.950,4.881,14.15,26.65,14.48和17.89 mg·mL-1,相应条件下表观油水分配系数(P)值分别为0.234,0.224,0.365,0.103,0.074,0.049,0.034,0.034和0.035.结论 黄芩苷的平衡溶解度在酸性及中性条件下受温度影响很小,在碱性条件下随着温度升高而增加;黄芩苷在酸性条件下的P值比在碱性条件下大,Pmax=0.363(T=25 ℃,pH=2),Pmax=0.365(T=37 ℃,pH=4),随着碱性的增加P值变化不明显,且温度对黄芩苷的P值几乎无影响.
目的 攷察黃芩苷在不同pH值中的平衡溶解度與錶觀油水分配繫數,為製劑研究奠定基礎.方法 採用搖瓶-紫外分光光度法測定溫度為25和37 ℃時,黃芩苷在不同pH值燐痠鹽緩遲溶液中的平衡溶解度及在正辛醇/緩遲液體繫中的錶觀油水分配繫數.結果 25 ℃時,黃芩苷在pH=2.0,3.0,4.0,5.0,6.0,6.8,7.4,8.0和9.0緩遲液中的平衡溶解度分彆為0.032,0.034,0.119,0.873,3.329,12.96,11.49,4.605和11.87 mg·mL-1,相應條件下錶觀油水分配繫數(P)值分彆為0.363,0.244,0.292,0.137,0.057,0.046,0.036,0.028和0.029.37 ℃時,黃芩苷在pH=2.0,3.0,4.0,5.0,6.0,6.8,7.4,8.0和9.0緩遲液中的平衡溶解度分彆為0.028,0.048,0.095,0.950,4.881,14.15,26.65,14.48和17.89 mg·mL-1,相應條件下錶觀油水分配繫數(P)值分彆為0.234,0.224,0.365,0.103,0.074,0.049,0.034,0.034和0.035.結論 黃芩苷的平衡溶解度在痠性及中性條件下受溫度影響很小,在堿性條件下隨著溫度升高而增加;黃芩苷在痠性條件下的P值比在堿性條件下大,Pmax=0.363(T=25 ℃,pH=2),Pmax=0.365(T=37 ℃,pH=4),隨著堿性的增加P值變化不明顯,且溫度對黃芩苷的P值幾乎無影響.
목적 고찰황금감재불동pH치중적평형용해도여표관유수분배계수,위제제연구전정기출.방법 채용요병-자외분광광도법측정온도위25화37 ℃시,황금감재불동pH치린산염완충용액중적평형용해도급재정신순/완충액체계중적표관유수분배계수.결과 25 ℃시,황금감재pH=2.0,3.0,4.0,5.0,6.0,6.8,7.4,8.0화9.0완충액중적평형용해도분별위0.032,0.034,0.119,0.873,3.329,12.96,11.49,4.605화11.87 mg·mL-1,상응조건하표관유수분배계수(P)치분별위0.363,0.244,0.292,0.137,0.057,0.046,0.036,0.028화0.029.37 ℃시,황금감재pH=2.0,3.0,4.0,5.0,6.0,6.8,7.4,8.0화9.0완충액중적평형용해도분별위0.028,0.048,0.095,0.950,4.881,14.15,26.65,14.48화17.89 mg·mL-1,상응조건하표관유수분배계수(P)치분별위0.234,0.224,0.365,0.103,0.074,0.049,0.034,0.034화0.035.결론 황금감적평형용해도재산성급중성조건하수온도영향흔소,재감성조건하수착온도승고이증가;황금감재산성조건하적P치비재감성조건하대,Pmax=0.363(T=25 ℃,pH=2),Pmax=0.365(T=37 ℃,pH=4),수착감성적증가P치변화불명현,차온도대황금감적P치궤호무영향.
