国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2010年
12期
812-815,封3
,共5页
酸性成纤维细胞生长因子%细胞培养%干细胞%内皮祖细胞
痠性成纖維細胞生長因子%細胞培養%榦細胞%內皮祖細胞
산성성섬유세포생장인자%세포배양%간세포%내피조세포
Acid fibroblast growth factor%Cell culture%Stem cells%Endothelial progenitor cells
目的 观察酸性成纤维细胞生长因子(aFGF)对体外培养内皮祖细胞(EPCs)数量、功能及凋亡的影响.方法 密度梯度离心法获取人脐血单个核细胞(MNCs),培养6 d后,收集贴壁细胞流式细胞仪和激光共聚焦显微镜鉴定EPCs.并向贴壁细胞分别加入aFGF 2、5、10μg/L干预培养24 h,同时对作用效果最为显著的组(aFGF 5μg/L组)分别培养6、12、24、48 h,分别观察EPCs数量、增生、迁移、黏膜及凋亡状况,从而对其时效关系进行观察.结果 与对照组比较,不同浓度的aFGF可以显著提高EPCs的数量、生物学功能并抑制其凋亡(P<0.05);本研究5μg/L aFGF作用24 h时对EPCs数量、生物学功能及凋亡抑制的影响最为显著(P<0.05).结论 aFGF增加体外培养条件下EPCs的数目、改善其生物学功能并抑制其凋亡.
目的 觀察痠性成纖維細胞生長因子(aFGF)對體外培養內皮祖細胞(EPCs)數量、功能及凋亡的影響.方法 密度梯度離心法穫取人臍血單箇覈細胞(MNCs),培養6 d後,收集貼壁細胞流式細胞儀和激光共聚焦顯微鏡鑒定EPCs.併嚮貼壁細胞分彆加入aFGF 2、5、10μg/L榦預培養24 h,同時對作用效果最為顯著的組(aFGF 5μg/L組)分彆培養6、12、24、48 h,分彆觀察EPCs數量、增生、遷移、黏膜及凋亡狀況,從而對其時效關繫進行觀察.結果 與對照組比較,不同濃度的aFGF可以顯著提高EPCs的數量、生物學功能併抑製其凋亡(P<0.05);本研究5μg/L aFGF作用24 h時對EPCs數量、生物學功能及凋亡抑製的影響最為顯著(P<0.05).結論 aFGF增加體外培養條件下EPCs的數目、改善其生物學功能併抑製其凋亡.
목적 관찰산성성섬유세포생장인자(aFGF)대체외배양내피조세포(EPCs)수량、공능급조망적영향.방법 밀도제도리심법획취인제혈단개핵세포(MNCs),배양6 d후,수집첩벽세포류식세포의화격광공취초현미경감정EPCs.병향첩벽세포분별가입aFGF 2、5、10μg/L간예배양24 h,동시대작용효과최위현저적조(aFGF 5μg/L조)분별배양6、12、24、48 h,분별관찰EPCs수량、증생、천이、점막급조망상황,종이대기시효관계진행관찰.결과 여대조조비교,불동농도적aFGF가이현저제고EPCs적수량、생물학공능병억제기조망(P<0.05);본연구5μg/L aFGF작용24 h시대EPCs수량、생물학공능급조망억제적영향최위현저(P<0.05).결론 aFGF증가체외배양조건하EPCs적수목、개선기생물학공능병억제기조망.
Objective To investigate whether acid fibroblast growth factor (aFGF)can augument the number of endothelial progenitor cells ( EPCs), enhance their biological function and inhibit their apoptosis.Methods Mononuclear cells(MNCs) from human umbilical cord blood were isolated by Ficoll density gradient centrifugation,and cultured in vitro. After cultured six days, the attached cells were identified by flow cytometry and confocal laser scanning microscope. The cells were added with aFGF(2, 5, 10 μg/L) for 24 hours. Meanwhile, the attached cells in the group (aFGF 5 μg/L group)of the most obvious effects on EPCs was cultured for 6,12,24,48 h respectively ,accordingly, to explore the relationship between time and effect of aFGF 5 μg/L group. EPCs proliferation was assayed with CCK-8 kit assay. EPCs migration was assayed by modified Boyden chamber assay. EPCs adhesion assay was performed by replating cells on fibronectin-coated dishes,and then adherent cells were counted. Flow cytometry was uesd to detect cell apoptosis. Results Compared with control groop, aFGF can argument the number of EPCs and enhance the biological function of proliferation, migration, adhesion. In addition, aFGF can inhibit apoptosis of EPCs ( P ＜ 0. 05 ). The increase and inhibition ratio of apoptosis reached the maximum 24 h after administration of 5 μg/L( P ＜ 0.05 ). Conclusion The results of the present study define a novel mechanism of the action of aFGF: aFGF can augment the number of EPCs, enhance the functional activity and inhibit apoptosis in vitro.
