国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2008年
3期
154-155,160
,共3页
弧菌,副溶血性%药敏试验%弧菌显色培养基
弧菌,副溶血性%藥敏試驗%弧菌顯色培養基
호균,부용혈성%약민시험%호균현색배양기
Vibrio parahaemolyticus%Drug susceptibility test%Vibrio chromogenic medium
目的 研究弧菌显色培养基对副溶血性弧菌(Vp)的筛选性能及Vp在临床中的检出率和抗菌谱.方法 肠道病患者粪便样本经碱性蛋白胨水增菌后,接种于弧菌显色培养基上,对淡紫色菌落进行生化鉴定分析,得出弧菌显色培养基的选择性能;药敏实验采用K-B法.结果 802份粪便样本经碱性蛋白胨水增菌和弧菌显色培养基培养后,193份样本中长出淡紫色菌落.附加试验和生化反应符合Vp标准的菌株为178株,检出率为22.2%,弧菌显色培养基鉴定Vp的阳性预测值为92.0%.药敏结果显示,Vp对氨苄西林耐药率达82.0%,对Ⅰ代头孢菌素(头孢噻吩、头孢唑林)的耐药率为22.5%,而对Ⅲ代头孢菌素、氨基糖苷类、Ⅲ代喹诺酮类及其他抗生素的耐药率<5.0%.结论 弧菌显色培养基对肠道门诊患者Vp的检出简便快速,准确度较高;需要时可选用第Ⅲ代喹诺酮类或头孢菌素、氨基糖苷类和哌拉西林等抗生素进行治疗.
目的 研究弧菌顯色培養基對副溶血性弧菌(Vp)的篩選性能及Vp在臨床中的檢齣率和抗菌譜.方法 腸道病患者糞便樣本經堿性蛋白胨水增菌後,接種于弧菌顯色培養基上,對淡紫色菌落進行生化鑒定分析,得齣弧菌顯色培養基的選擇性能;藥敏實驗採用K-B法.結果 802份糞便樣本經堿性蛋白胨水增菌和弧菌顯色培養基培養後,193份樣本中長齣淡紫色菌落.附加試驗和生化反應符閤Vp標準的菌株為178株,檢齣率為22.2%,弧菌顯色培養基鑒定Vp的暘性預測值為92.0%.藥敏結果顯示,Vp對氨芐西林耐藥率達82.0%,對Ⅰ代頭孢菌素(頭孢噻吩、頭孢唑林)的耐藥率為22.5%,而對Ⅲ代頭孢菌素、氨基糖苷類、Ⅲ代喹諾酮類及其他抗生素的耐藥率<5.0%.結論 弧菌顯色培養基對腸道門診患者Vp的檢齣簡便快速,準確度較高;需要時可選用第Ⅲ代喹諾酮類或頭孢菌素、氨基糖苷類和哌拉西林等抗生素進行治療.
목적 연구호균현색배양기대부용혈성호균(Vp)적사선성능급Vp재림상중적검출솔화항균보.방법 장도병환자분편양본경감성단백동수증균후,접충우호균현색배양기상,대담자색균락진행생화감정분석,득출호균현색배양기적선택성능;약민실험채용K-B법.결과 802빈분편양본경감성단백동수증균화호균현색배양기배양후,193빈양본중장출담자색균락.부가시험화생화반응부합Vp표준적균주위178주,검출솔위22.2%,호균현색배양기감정Vp적양성예측치위92.0%.약민결과현시,Vp대안변서림내약솔체82.0%,대Ⅰ대두포균소(두포새분、두포서림)적내약솔위22.5%,이대Ⅲ대두포균소、안기당감류、Ⅲ대규낙동류급기타항생소적내약솔<5.0%.결론 호균현색배양기대장도문진환자Vp적검출간편쾌속,준학도교고;수요시가선용제Ⅲ대규낙동류혹두포균소、안기당감류화고랍서림등항생소진행치료.
Objective To study the feasibility of vibrio chromogenic medium isolating Vibrio parahaemolyticus(Vp)and the clinical Vp isolation and resistance rate of antibiotics.Method After bacterial enrichment in alkaline peptone water,stool samples of patients were inoculated in vibrio colored medium,and Vp tradidonal biochemical tests were applied later to identify the purple colonies on the plate.The resistance to antibiotics was carried out by K-B test.Results In 802 samples of diarrhea-out patient,there were 193 samples growing purple colonies on vibrio colored medium,in which 178 strains were identified as Vp by traditional biochemical tests. The clinical isolating rate of Vp was 22.2%and the positive predictive value for Vp of the colored medium was 92.0%.The rates of resistance to ampicillin.cefalothin and cefazolin were 82.0%.22.5%and 22.5% respectively.The rates of resistance to other drugs used in this study were all below 5.0%.Conclusions The vibrio colored medium on isolating Vp is fast,simple and has good accuracy.The third generation of quinolones and cephalosporin,and the aminoglycosides could be chosen to treat Vp infection when needed.
