CAJ | 학술논문

目的观察颈外动脉移植骨髓间充质干细胞( BMSC)对脑挫伤大鼠神经功能恢复及学习记忆的影响.方法 10只SD大鼠随机数字表法分为外伤性颅脑损伤(TBI)组和BMSC移植组.采用自由落体制作大鼠皮质运动区脑挫伤模型,将培养纯化并鉴定的BMSC于术后当天经颈外动脉移植人体内；术后1d、3d、7d、15d行改良神经功能缺失(NSS)评分,并于15d进行水迷宫测试以观察动物学习记忆能力.15d时取脑组织用免疫荧光技术检测hochest标记的移植BMSC在体内存活、迁移情况.结果脑外伤组大鼠损伤后即出现不同程度抽搐、瘫痪、平衡功能缺失,神经功能缺损评分明显升高.而BMSC移植组的NSS评分至第7天后[(6.5±1.19)分]与单纯手术组(TBI)[ (8.75 ±0.68)分]比较明显减少(P＜0.01).第15天进行水迷宫测试发现BMSC移植组逃避潜伏期[(20.48±2.29)s]显著优于TBI组[(85.93±47.48)s,P＜0.01].BMSC组在目标象限停留时间百分比[(28.62±1.72)％]和路程百分比[(29.05±3.08)％]明显高于TBI组[(19.37±2.81)％,(21.78±3.06)％],均差异有统计学意义(P＜0.01).BMSC组穿越站台次数[(8.00±2.45)次]和TBI组[(2.00±1.87)次]相比,差异也有统计学意义(P＜0.01).免疫荧光检测显示,移植BMSC能在宿主脑组织存活,并向四周迁移.结论颈外动脉移植BMSC能在挫伤脑组织存活、迁移,并改善脑挫伤大鼠神经功能和学习记忆能力.
목적 관찰경외동맥이식골수간충질간세포( BMSC)대뇌좌상대서신경공능회복급학습기억적영향.방법 10지SD대서수궤수자표법분위외상성로뇌손상(TBI)조화BMSC이식조.채용자유락체제작대서피질운동구뇌좌상모형,장배양순화병감정적BMSC우술후당천경경외동맥이식인체내；술후1d、3d、7d、15d행개량신경공능결실(NSS)평분,병우15d진행수미궁측시이관찰동물학습기억능력.15d시취뇌조직용면역형광기술검측hochest표기적이식BMSC재체내존활、천이정황.결과 뇌외상조대서손상후즉출현불동정도추휵、탄탄、평형공능결실,신경공능결손평분명현승고.이BMSC이식조적NSS평분지제7천후[(6.5±1.19)분]여단순수술조(TBI)[ (8.75 ±0.68)분]비교명현감소(P＜0.01).제15천진행수미궁측시발현BMSC이식조도피잠복기[(20.48±2.29)s]현저우우TBI조[(85.93±47.48)s,P＜0.01].BMSC조재목표상한정류시간백분비[(28.62±1.72)％]화로정백분비[(29.05±3.08)％]명현고우TBI조[(19.37±2.81)％,(21.78±3.06)％],균차이유통계학의의(P＜0.01).BMSC조천월참태차수[(8.00±2.45)차]화TBI조[(2.00±1.87)차]상비,차이야유통계학의의(P＜0.01).면역형광검측현시,이식BMSC능재숙주뇌조직존활,병향사주천이.결론 경외동맥이식BMSC능재좌상뇌조직존활、천이,병개선뇌좌상대서신경공능화학습기억능력.
Objective To study the effect of bone mesenchymal stem cells (BMSC) transplantation into traumatic brain injury(TBI) rats by the external carotid artery on neurological function and learning and memory.Methods Ten adult SD rats were randomly divided into TBI group ( n =5 ) and BMSC transplantation group ( n=5).Feeney free falling method was used to establish TBI models.The experimental rats were administrated with BMSC via external carotid artery (ECA),while TBI rats were injected with sterile liquid medium of equal volume via right ECA.Neurological function were evaluated according to the modified neurological severity score (NSS) at 1,3,7,15 days.Morris water maze test was used to observe the animal capabilities of place navigation and space exploration at 15 days,then animals were sacrificed.Survival and migration of implanted BMSC in brains under fluorescence microscope. Result After traumatic brain,varying degrees convulsions,paralysis,loss of balance function in rats were found.Compared with TBI group,BMSC transplantation decreased significantly NSS (P ＜0.01 ).BMSC transplantation significantly decreased on escape latency ( ( 20.48 ± 2.29 ) s ) than the TBI group ( ( 85.93 ± 47.48 ) s) (P ＜ 0.01 ).Moreover,BMSC group in the target quadrant dwell time ( ( 28.62 ± 1.72) ％ )and distance ( (29.05 ± 3.08 )％ ) as well as the number of passing the platform (8.00 ± 2.45 ) were significantly higher than the TBI group ( ( 19.37 ± 2.81 ) ％,(21.78 ± 3.06) ％,(2.00 ± 1.87) respectively,P ＜ 0.01 ).Transplanted BMSC could survive and migrate around injury brain through Hochest mark immunofluorescence.Conclusion BMSC can survive and migrate around injury brain by transplantation of external carotid artery,which results in a significant neurological function improvement and learning and memory increase in rats with traumatic brain injury.