中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
2期
169-172
,共4页
王治东%葛常辉%许望翔%詹轶群%汤立宣%曹萌萌%杨晓明
王治東%葛常輝%許望翔%詹軼群%湯立宣%曹萌萌%楊曉明
왕치동%갈상휘%허망상%첨질군%탕립선%조맹맹%양효명
TLR5%CBLB502蛋白%辐射防护
TLR5%CBLB502蛋白%輻射防護
TLR5%CBLB502단백%복사방호
Toll-like receptor 5%CBLB502 protein%Radiation protection
目的 原核表达并纯化TLR5受体的激动剂CBLB502蛋白,验证其辐射防护作用.方法 采用pET28b原核表达系统表达CBLB502蛋白,利用Ni-NTA亲和层析柱亲和层析纯化CBLB502蛋白,经报告基因检测方法结合动物实验评价纯化蛋白活性.结果 成功表达并纯化CBLBS02蛋白,纯度可达95%以上,能够激活NF-KB报告基因,可以提高9 Gy照射小鼠存活率,对照组小鼠全都死亡.CBLB502蛋白预防组全部存活.结论 成功表达并纯化CBLB502蛋白,并验证其对小鼠的辐射防护作用.
目的 原覈錶達併純化TLR5受體的激動劑CBLB502蛋白,驗證其輻射防護作用.方法 採用pET28b原覈錶達繫統錶達CBLB502蛋白,利用Ni-NTA親和層析柱親和層析純化CBLB502蛋白,經報告基因檢測方法結閤動物實驗評價純化蛋白活性.結果 成功錶達併純化CBLBS02蛋白,純度可達95%以上,能夠激活NF-KB報告基因,可以提高9 Gy照射小鼠存活率,對照組小鼠全都死亡.CBLB502蛋白預防組全部存活.結論 成功錶達併純化CBLB502蛋白,併驗證其對小鼠的輻射防護作用.
목적 원핵표체병순화TLR5수체적격동제CBLB502단백,험증기복사방호작용.방법 채용pET28b원핵표체계통표체CBLB502단백,이용Ni-NTA친화층석주친화층석순화CBLB502단백,경보고기인검측방법결합동물실험평개순화단백활성.결과 성공표체병순화CBLBS02단백,순도가체95%이상,능구격활NF-KB보고기인,가이제고9 Gy조사소서존활솔,대조조소서전도사망.CBLB502단백예방조전부존활.결론 성공표체병순화CBLB502단백,병험증기대소서적복사방호작용.
Objective To obtain the purified CBLB502 protein as an agonist of Toll like receptor 5 in prokaryocytes and to validate its radiation protective effect.Methods pET28b expression system was used to express CBLB502 protein,and the Ni-NTA agarose and purification column was used to purify CBLB502 protein.The radiation proective effect of CBLB502 was evaluated by NF-kB reporter gene assay and mice experiments.Results CBLB502 protein was expressed and purified,which could activate NFkB reporter gene in vitro and improved the livability of irradiated mice by 9 Gy γ-ray.Conclusions CBLB502 protein could be successfully expressed and purified using prokaryotic expression system.It has the excellent radiation protective effect in mice.
