CAJ | 학술논문

目的通过分离并鉴定具有不同辐射抗拒的人鼻咽癌细胞株CNE-2R与CNE-2的差异表达蛋白,探讨与鼻咽癌细胞辐射抗拒相关的分子机制.方法分别提取鼻咽癌辐射抗拒细胞株CNE-2R及其亲本细胞株CNE-2的总蛋白,采用双向凝胶电泳分离,经软件分析识别差异表达蛋白点,应用基质辅助激光解析电离飞行时间串联质谱技术(MALDI-TOF-MS)鉴定差异蛋白质.结果两种不同辐射敏感性的鼻咽癌细胞株CNE-2R和CNE-2中,共筛选出差异表达明显的蛋白质点有32个,其中11个蛋白质被鉴定成功,在CNE-2R中上调的蛋白有3个,下调的蛋白有8个.结论不同辐射敏感性的鼻咽癌细胞的差异表达蛋白,主要涉及调节凋亡、DNA损伤与修复、细胞周期调控、RNA转录、细胞信号转导、细胞骨架组成及辐射应激反应等多个方面.
목적 통과분리병감정구유불동복사항거적인비인암세포주CNE-2R여CNE-2적차이표체단백,탐토여비인암세포복사항거상관적분자궤제.방법 분별제취비인암복사항거세포주CNE-2R급기친본세포주CNE-2적총단백,채용쌍향응효전영분리,경연건분석식별차이표체단백점,응용기질보조격광해석전리비행시간천련질보기술(MALDI-TOF-MS)감정차이단백질.결과 량충불동복사민감성적비인암세포주CNE-2R화CNE-2중,공사선출차이표체명현적단백질점유32개,기중11개단백질피감정성공,재CNE-2R중상조적단백유3개,하조적단백유8개.결론 불동복사민감성적비인암세포적차이표체단백,주요섭급조절조망、DNA손상여수복、세포주기조공、RNA전록、세포신호전도、세포골가조성급복사응격반응등다개방면.
Objective To discover radioresistance-associated proteins by performing comparative proteomic analysis on nasopharyngeal carcinoma cell lines.Methods The total proteins were extracted from radioresistant human nasopharyngeal carcinoma cell line CNE-2R and its parental cell line CNE-2,respectively.These proteins were separated by high quality two-dimensional polyacrylamide gel electrophoresis (2-DE) and then the 2-DE profiles were screened for differentially expressed protein spots by the Image Master 5.0 software.Those spots were identified by a matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.Results 32 significantly differentially expressed protein spots were screened in two different radiosensitivity cell lines and 11 proteins were identified by tandem mass spectrometry,among which 3 proteins were up-regulated in radioresistant human nasopharyngcal carcinoma cell line CNE-2R and the other 8 proteins were down-regulated.Conclusions The differentially expressed proteins of nasopharyegeal carcinoma cells with different radiosensitivity were mainly involved in apoptosis regulation,DNA damage and repair,cell cycle regulation,RNA transcription,cell signaling,cytoskeleton formation and radiation stress responses.