CAJ | 학술논문

目的比较原发性气管腺样囊性癌患者与健康体检者血清蛋白组学,通过两组间差异蛋白的筛选为原发性气管腺样囊性癌的特异性蛋白标记物的寻找奠定基础.方法应用表面增强激光解析电离飞行时间质谱技术,分析原发性气管腺样囊性癌组和对照组血清,获得蛋白表达图谱,用Biomarker Wizard软件分析得到两组间差异蛋白.结果原发性气管腺样囊性癌组与对照组比较,存在31个差异蛋白(P＜0.01),其中16个蛋白在原发性气管腺样囊性癌中高表达,15个蛋白低表达.结论 ①原发性腺样囊性癌血清与对照组比较,存在明显差异蛋白,为进一步寻找有诊断价值的特异性血清蛋白标记物提供了依据和研究方向；②血清差异蛋白的出现进而血清蛋白标记物的确定为该病发病机制的明确以及由此针对发病机制产生的新的治疗方法奠定了基础.
목적 비교원발성기관선양낭성암환자여건강체검자혈청단백조학,통과량조간차이단백적사선위원발성기관선양낭성암적특이성단백표기물적심조전정기출.방법 응용표면증강격광해석전리비행시간질보기술,분석원발성기관선양낭성암조화대조조혈청,획득단백표체도보,용Biomarker Wizard연건분석득도량조간차이단백.결과 원발성기관선양낭성암조여대조조비교,존재31개차이단백(P＜0.01),기중16개단백재원발성기관선양낭성암중고표체,15개단백저표체.결론 ①원발성선양낭성암혈청여대조조비교,존재명현차이단백,위진일보심조유진단개치적특이성혈청단백표기물제공료의거화연구방향；②혈청차이단백적출현진이혈청단백표기물적학정위해병발병궤제적명학이급유차침대발병궤제산생적신적치료방법전정료기출.
Objective To compare the profiles of serum proteomics in patients with primary tracheal adenoid cystic carcinoma and those without this disease,aiming at identifying significant biomarkers of primary tracheal adenoid cystic carcinoma.Methods The profiles of serum proteomics in patients with primary tracheal adenoid cystic carcinoma and controls were obtained by the technique of SELDI-TOF-MS,and the differential expression protein were identified through the analysis of Biomarker Wizard software.Results 31 differential expression protein were found out across the two groups.16 protein were expressed higher in primary tracheal adenoid cystic carcinoma,and 15 were expressed lower.Conclusions ①Differential expressed protein exists in primary tracheal adenoid cystic carcinoma,and this provides the evidence and orients for the research of the specific biomarkers in the adenoid cystic carcinoma.②The outcome of the differential protein and the definitude of the biomarkers lay a foundation for the detection of the mechanism in the development of adenoid cystic carcinoma,and furthermore for the emergency of the new treatment according to the mechanism.