CAJ | 학술논문

针对目前胃肠间质瘤诊断的临床和病理常规工作中普遍存在标本固定不足的问题,应该强调标本固定、免疫组织化学(免疫组化)和基因突变检测的规范化.笔者根据自己的工作经验,对固定不佳标本的免疫组化和c-kit基因检测的常规方法作某些调整,以改善或提高检测效果,这对参与胃肠间质瘤诊断工作的临床医生、病理医生以及免疫组化和基因检测实验室的工作人员有一定参考价值.
침대목전위장간질류진단적림상화병리상규공작중보편존재표본고정불족적문제,응해강조표본고정、면역조직화학(면역조화)화기인돌변검측적규범화.필자근거자기적공작경험,대고정불가표본적면역조화화c-kit기인검측적상규방법작모사조정,이개선혹제고검측효과,저대삼여위장간질류진단공작적림상의생、병리의생이급면역조화화기인검측실험실적공작인원유일정삼고개치.
The most common problem in the diagnosis of gastrointestinal stromal tumor (GIST) is inadequate specimen fixation. The paper focused on specimen fixation and standardized protocol in immunohistochemistry staining and gene mutation detection.We have adjusted some procedure used in immunohistochemsitry staining and c-kit gene detection to improve the quality of inadequately fixed specimen.It maybe useful for clinicians,pathologists and technicians working in immunohischemistry labs and gene detection labs.