CAJ | 학술논문

目的：观察谷氨酰胺(Gln)对短肠综合征大鼠残留小肠、结肠形态的影响。　方法：23只雄性SD大鼠切除80%小肠，随机分为三组：饮食组(n=8)大鼠术后自由进食；全胃肠外营养(TPN)组(n=8)输TPN标准液；Gln组(n=7)输TPN+Gln液；正常大鼠8只，作为正常对照组。术后第7天，称体重，取残留空肠、回肠、结肠进行组织学检查(包括光镜和电镜)。　结果：饮食组和Gln组术前体重无明显差异，但术后体重有明显差异；饮食组空肠粘膜绒毛高度(VH)和粘膜厚度(MT)、回肠粘膜的VH均明显大于正常组；TPN组空肠粘膜VH、MT明显小于正常组；回肠粘膜隐窝深度(CD)、MT亦明显小于正常组；Gln组空肠和回肠粘膜VH、CD和MT明显大于TPN组；饮食组结肠MT明显大于正常组，Gln组结肠MT明显大于TPN组。　结论：80%小肠切除后，残留小肠发生代偿性改变，食物刺激是残留小肠代偿的重要因素；但这种代偿不完全，TPN可维持机体体重，但可引起残留小肠粘膜萎缩；Gln能阻止TPN引起残留小肠粘膜萎缩，促进残留小肠代偿；同时Gln还促使结肠粘膜增生。
목적：관찰곡안선알(Gln)대단장종합정대서잔류소장、결장형태적영향。　방법：23지웅성SD대서절제80%소장，수궤분위삼조：음식조(n=8)대서술후자유진식；전위장외영양(TPN)조(n=8)수TPN표준액；Gln조(n=7)수TPN+Gln액；정상대서8지，작위정상대조조。술후제7천，칭체중，취잔류공장、회장、결장진행조직학검사(포괄광경화전경)。　결과：음식조화Gln조술전체중무명현차이，단술후체중유명현차이；음식조공장점막융모고도(VH)화점막후도(MT)、회장점막적VH균명현대우정상조；TPN조공장점막VH、MT명현소우정상조；회장점막은와심도(CD)、MT역명현소우정상조；Gln조공장화회장점막VH、CD화MT명현대우TPN조；음식조결장MT명현대우정상조，Gln조결장MT명현대우TPN조。　결론：80%소장절제후，잔류소장발생대상성개변，식물자격시잔류소장대상적중요인소；단저충대상불완전，TPN가유지궤체체중，단가인기잔류소장점막위축；Gln능조지TPN인기잔류소장점막위축，촉진잔류소장대상；동시Gln환촉사결장점막증생。
Objectives:To investigate the effect of glutamine on the shape of residual intestine and colon in rats with short bowel syndrome.　Methods:23 male Sprague-Dawley rats,underwent a 80% small bowel resection,were randomly divided into three groups:food group(n=8) rats,fed rat chow and water libitum after operation;TPN group(n=8),infused with Gln-supplied TPN;and normal control group.On seventh day after operation,rats were weighted and remaining jejunum、remaining ileum and colon were harvested for histological observation(light microscopy and electron microscopy).　Results:There was significant difference in rat average weight between food group and Gln group after operation.Jejunal mucosal villus height(VH) and mucosal thickness(MT) and ileal mucosal VH in food group were significantly increased than those in control group.Jejunal mucosal VH and MT in control group were significantly higher than in TPN group.Ileal mucosal crypt depth(CD) and MT in control group were also significantly higher than in TPN group.Jejunal and ileal mucosal VH、CD and MT in Gln group were significantly higher than in TPN group.Colonic MT in food group was significantly higher than in control group.Colonic MT in Gln group was significantly bigger than in TPN group.　Conclusions:After 80% intestinal resection,the remaining intestine can develop the adaptation,but the adaptation is incomplete.TPN therapy can maintain body weight,but only TPN can not result in the adaption.Gln-supplied TPN can stop the remaining itestinal mucosal atrophy,and promote the remaining intestinal adaptation and colonic mucosal hypertrophy.