CAJ | 학술논문

为应用脉冲场凝胶电泳(PFGE)从分子水平上对禽源沙门氏菌之间的差别进行分析和研究,本研究采用自动微生物鉴定仪对12株疑似鼠伤寒沙门氏菌和15株疑似鸡白痢沙门氏菌进行了鉴定.以限制性核酸内切酶Xba I对其全基因组DNA进行酶切、PFGE分型,Info Quest FP聚类分析软件对电泳结果进行分析.结果显示,共鉴定出11株鼠伤寒沙门氏菌、1株圣保罗沙门氏菌、6株阿姆斯特丹沙门氏菌、5株亚拉巴马沙门氏菌、1株鸡白痢沙门氏菌、2株纽波特沙门氏菌和2株肠炎沙门氏菌,一共分为12个PFGE型.实验结果表明,江苏地区存在有不常见的沙门氏菌血清型,同种血清型之间的基因型差别较小(相似值为0.85～1),不同血清型之间差别较大(相似值为0.45～0.70).PFGE能从分子水平上对禽源沙门氏菌的基因组DNA进行分型.
위응용맥충장응효전영(PFGE)종분자수평상대금원사문씨균지간적차별진행분석화연구,본연구채용자동미생물감정의대12주의사서상한사문씨균화15주의사계백리사문씨균진행료감정.이한제성핵산내절매Xba I대기전기인조DNA진행매절、PFGE분형,Info Quest FP취류분석연건대전영결과진행분석.결과현시,공감정출11주서상한사문씨균、1주골보라사문씨균、6주아모사특단사문씨균、5주아랍파마사문씨균、1주계백리사문씨균、2주뉴파특사문씨균화2주장염사문씨균,일공분위12개PFGE형.실험결과표명,강소지구존재유불상견적사문씨균혈청형,동충혈청형지간적기인형차별교소(상사치위0.85～1),불동혈청형지간차별교대(상사치위0.45～0.70).PFGE능종분자수평상대금원사문씨균적기인조DNA진행분형.
To investigate the molecular diversity of the avian Salmonella, 12 S. Typhimurium strains and 12 S. Pullorum strains were identified by automatic microbiological assay and their genomic DNA were digested by restriction endonuclease Xba I and typed by Pulsed-Field Gel Electrophoresis (PFGE). The electrophoresis profiles were analyzed by cluster analysis software-Info Quest FP. The results showed that there were 12 PFGE patterns, which displayed 11 strains S .typhimurium, 1 strain S. Saint paul,6 strains S. Amsterdam, 5 strains S. Alabama, 1 strain S. Pullorum, 2 strains S. Newport and 2 strains S. Enteritidis. The result indicated that there was uncommon serotype Salmonella existed in Jiangsu province. The diversity among the same serotype Salmonella were not obvious (similarity score 0.85-1), but the obvious diversity were found in the different serotype (similarity score 0.45-0.70). Therefore, PFGE could be used effectively to type the genomic DNA of avian Salmonella at molecular level.