CAJ | 학술논문

应用亲和型生物传感器Iasys实时监测白细胞介素-2(IL-2)与其可溶性受体(Sil-2R)之间的相互作用,并进一步揭示IL-2/sIL-2R在体内的作用机理.将sIL-2R进行生物素标记后,利用生物素-亲和素系统将其固定于Iasys生物传感器的生物素样品池表面,向样品池中加入IL-2,通过检测由IL-2与固定于样品池表面的sIL-2R结合而引起的传感基片表面共振光角度的变化来研究IL-2和sIL-2R之间的相互作用.IL-2可与sIL-2R在体外发生特异性结合.Iasys生物传感器是一种研究生物大分子间相互作用的理想工具,为细胞因子/受体体系的相互作用研究提供了有效的方法和新的思路.
응용친화형생물전감기Iasys실시감측백세포개소-2(IL-2)여기가용성수체(Sil-2R)지간적상호작용,병진일보게시IL-2/sIL-2R재체내적작용궤리.장sIL-2R진행생물소표기후,이용생물소-친화소계통장기고정우Iasys생물전감기적생물소양품지표면,향양품지중가입IL-2,통과검측유IL-2여고정우양품지표면적sIL-2R결합이인기적전감기편표면공진광각도적변화래연구IL-2화sIL-2R지간적상호작용.IL-2가여sIL-2R재체외발생특이성결합.Iasys생물전감기시일충연구생물대분자간상호작용적이상공구,위세포인자/수체체계적상호작용연구제공료유효적방법화신적사로.
The affinity biosensor IAsys was used to monitor the interaction of interleukin-2(IL-2)and its soluble receptor in real-time in order to reveal the interaction mechanism of IL-2 and its soluble receptor in vivo.Biotinlabeled sIL-2R was immobilized on the surface of IAsys cuvette,then IL-2 Was added into the cuvette.The interaction between IL-2 and sIL-2R was studied by detecting the change in the refractive index on the biosensor Surface.The experimental results show that IL-2 could bind to sIL-2R specifically.IAsys biosensor has been known independently as surface sensitive analytical device capable of on-line monitoring of interfacial reactions between biomolecules.It provides an effective method and new idea for the studv of interaction between cytokine and receptor.