CAJ | 학술논문

从植物根际的土壤样品中分离筛选出两株高效解磷细菌P_9和P_(28),分别被鉴定为粘质沙雷氏菌和产气肠杆菌.研究了不同环境条件对解磷能力的影响,结果表明,P_(28)耐 3%NaCl(w/v,下同)、28℃是两菌的最适温度、在初始pH 5.5～10.0条件下培养时能够高效溶解Ca_3(PO_4)_2.盆栽试验结果表明,单接种、双接种解磷菌处理的玉米株高、植株干重和磷含量显著高于不接菌对照;单接种P_9处理的玉米明显强于单接种P_(28)处理;将有机肥(M)和磷矿石(RP)作为载体和解磷菌一同混合施入土壤的处理,玉米苗干重,磷含量较单施解磷菌显著增加.
종식물근제적토양양품중분리사선출량주고효해린세균P_9화P_(28),분별피감정위점질사뢰씨균화산기장간균.연구료불동배경조건대해린능력적영향,결과표명,P_(28)내 3%NaCl(w/v,하동)、28℃시량균적최괄온도、재초시pH 5.5～10.0조건하배양시능구고효용해Ca_3(PO_4)_2.분재시험결과표명,단접충、쌍접충해린균처리적옥미주고、식주간중화린함량현저고우불접균대조;단접충P_9처리적옥미명현강우단접충P_(28)처리;장유궤비(M)화린광석(RP)작위재체화해린균일동혼합시입토양적처리,옥미묘간중,린함량교단시해린균현저증가.
Two efficient phosphate-solubilizing bacteria(PSBs)were isolated from plant rhizosphere soil samples,and were identified as Serratia marcescens P_9and Enterobacter aerogenes P_(28).We measured quantitatively phosphate solubilization activities under various environmental conditions,28℃ was their optimum temperature,P_(28) was able to withstand high concentration of NaCl upto 3%(w/v,The same below),and a wide range of initial pH from 5.5 to 10.0 while hydrolyzing phosphate compounds actively.Pot experiment showed that plant height,dry mass and phosphorus content was significantly higher with single-inoculation,dual inoculation PSBs treatments than CK;these indicators of corn only applying P_9 agent were better than applying P_(28) agent only;Applying PSBs agent with manure and rock phosphate as a carrier could significantly increase the seedlingdry weight,phosphorus accumulation,in comparing with applying PSBs agent alone.