CAJ | 학술논문

目的:研究强脉冲光(intense pulsed light,IPL)照射对皮肤成纤维细胞分泌TGF-β1的影响及JNK抑制剂SP600125的干预作用.方法:利用包皮环切术切除的包皮组织体外分离、培养原代成纤维细胞.然后,将成纤维细胞分为两组试验:第1组为IPL治疗组,用能量密度分别为0(阴性对照),10,18,27,36和36 J/cm2×2(能量密度为36 J/cm2的IPL照射两次)的IPL照射;第2组为IPL+抑制剂组,包括IPL(对照)和IPL+SP600125(JNK抑制剂)两亚组,在加入抑制剂2 h后,用能量密度为36 J/cm2的IPL照射.48 h后,采用ELISA法检测两组细胞培养上清液(culture supernatants, CS)中TGF-β1的浓度.结果:IPL治疗组CS中TGF-β1的浓度在10,18,27及36 J/cm2分别与阴性对照组相比较均减少,而在36 J/cm2×2时与阴性对照相比较增高;IPL+抑制剂组CS中TGF-β1的浓度IPL+SP600125组与对照相比较减少(P<0.05).结论:强脉冲光在较低能量密度时抑制皮肤成纤维细胞TGF-β1的分泌,较高能量密度时能促进TGF-β1的分泌;在IPL影响成纤维细胞分泌TGF-β1的过程中,JNK抑制剂起抑制作用,IPL可能通过JNK途径上调TGF-β1的分泌.
목적:연구강맥충광(intense pulsed light,IPL)조사대피부성섬유세포분비TGF-β1적영향급JNK억제제SP600125적간예작용.방법:이용포피배절술절제적포피조직체외분리、배양원대성섬유세포.연후,장성섬유세포분위량조시험:제1조위IPL치료조,용능량밀도분별위0(음성대조),10,18,27,36화36 J/cm2×2(능량밀도위36 J/cm2적IPL조사량차)적IPL조사;제2조위IPL+억제제조,포괄IPL(대조)화IPL+SP600125(JNK억제제)량아조,재가입억제제2 h후,용능량밀도위36 J/cm2적IPL조사.48 h후,채용ELISA법검측량조세포배양상청액(culture supernatants, CS)중TGF-β1적농도.결과:IPL치료조CS중TGF-β1적농도재10,18,27급36 J/cm2분별여음성대조조상비교균감소,이재36 J/cm2×2시여음성대조상비교증고;IPL+억제제조CS중TGF-β1적농도IPL+SP600125조여대조상비교감소(P<0.05).결론:강맥충광재교저능량밀도시억제피부성섬유세포TGF-β1적분비,교고능량밀도시능촉진TGF-β1적분비;재IPL영향성섬유세포분비TGF-β1적과정중,JNK억제제기억제작용,IPL가능통과JNK도경상조TGF-β1적분비.
Objective To determine the influence of intense pulsed light (IPL) on the secretion of TGF-β1 in cultured human fibroblasts and the intervention of JNK inhibitor.Methods The callan foreskin fibroblasts were cultured and divided into 2 groups. In the IPL treatment group, cells were irradiated with IPL with fluences of0 (negative control), 10, 18, 27, 36, and 36 J/cm2 × 2 (irradiated with IPL with fluences of 36 J/cm2 twice). In the IPL + inhibitor group, cells were irradiated with IPL with fluences of 36 J/cm2 after incubation with the inhibitor SP600125 for 2 h. TGF-β1 in the culture supernatant was evaluated 48 h after the irradiation using enzyme-linked immunosorbent assay. Results Compared with the negative control, TGF-β1 in the culture supernatant decreased at the IPL irradiation of 10, 18, 27, and 36 J / cm2, whereas TGF-β1 increased at the IPL irradiation of 36 J/cm2× 2. In the IPL + inhibitor group, the concentration of TGF-β1 in the culture supernatant decreased compared with the controls (P<0.05). Conclusion IPL can suppress the secretion of TGF-β1 at the lower fluence and promote the secretion at a higher fluence. JNK inhibitor may play an inhibitive role when IPL regulates the TGF-β1 secretion in cultured human fibroblasts. IPL may stimulate TGF-β1 secretion of the fibroblast cells in human skin via JNK signal pathway.