CAJ | 학술논문

目的:探讨应激对大鼠肠道菌群及血清促肾上腺皮质激素释放激素(CRF)的影响及双歧杆菌对应激大鼠肠道功能的调节作用. 方法:50只SD大鼠随机分为5组:正常对照组、压力实验组、双歧杆菌干预组、思密达干预组、双歧杆菌+思密达共同干预组.采用WAS(water avoidance stress)避水实验构建大鼠应激模型,以三糖为探针,衍生化毛细管气相色谱法测定大鼠尿液中三种糖的浓度,以三氯蔗糖/甘露醇(S/M)评价大鼠肠道通透性;取大鼠新鲜粪便,用选择性培养基平皿计数法检测大鼠粪便菌群中几种代表性菌种的数量;取肠系膜淋巴结(MLN)培养后测定细菌移位率;用酶联免疫法测定大鼠血清中CRF和促肾上腺皮质激素(ACTH)的含量. 结果:与正常对照组相比,压力实验组大鼠粪便中以大肠杆菌杆菌为主的条件致病菌数量增多(7.347±0.277 vs 7.078±0.229,P<0.05);24 h尿液中甘露醇量升高(5.097%±0.453% vs 4.718%±0.399%,P<0.05),MLN细菌移位率升高(40% vs 10%,P<0.05);CRH(300.8 ng/L ±34.3 ng/L vs 267.0 ng/L±32.3 ng/L,P<0.05),ACTH (6.79 ng/L±0.65 1 ng/L vs 5.68 ng/L±0.799 ng/L,P<0.05)水平升高.与压力实验组相比,双歧杆菌干预组大肠杆菌(7.044±0.281vs 7.347±0.277,P<0.05)、类杆菌(9.075±0.393 vs 9.485±0.306,P<0.05)数量显著下降,细菌移位率下降(10% vs 40%,P<0.05);ACTH水平下降(5.92 ng/L±0.477 ng/L vs 6.79 ng/L±0.651 ng/L,P<0.05). 结论:在慢性应激条件下,大鼠出现肠道菌群失调、肠道通透性升高、神经内分泌处于应激状态的现象,双歧杆菌能够缓解慢性应激所导致的上述现象.
목적:탐토응격대대서장도균군급혈청촉신상선피질격소석방격소(CRF)적영향급쌍기간균대응격대서장도공능적조절작용. 방법:50지SD대서수궤분위5조:정상대조조、압력실험조、쌍기간균간예조、사밀체간예조、쌍기간균+사밀체공동간예조.채용WAS(water avoidance stress)피수실험구건대서응격모형,이삼당위탐침,연생화모세관기상색보법측정대서뇨액중삼충당적농도,이삼록자당/감로순(S/M)평개대서장도통투성;취대서신선분편,용선택성배양기평명계수법검측대서분편균군중궤충대표성균충적수량;취장계막림파결(MLN)배양후측정세균이위솔;용매련면역법측정대서혈청중CRF화촉신상선피질격소(ACTH)적함량. 결과:여정상대조조상비,압력실험조대서분편중이대장간균간균위주적조건치병균수량증다(7.347±0.277 vs 7.078±0.229,P<0.05);24 h뇨액중감로순량승고(5.097%±0.453% vs 4.718%±0.399%,P<0.05),MLN세균이위솔승고(40% vs 10%,P<0.05);CRH(300.8 ng/L ±34.3 ng/L vs 267.0 ng/L±32.3 ng/L,P<0.05),ACTH (6.79 ng/L±0.65 1 ng/L vs 5.68 ng/L±0.799 ng/L,P<0.05)수평승고.여압력실험조상비,쌍기간균간예조대장간균(7.044±0.281vs 7.347±0.277,P<0.05)、류간균(9.075±0.393 vs 9.485±0.306,P<0.05)수량현저하강,세균이위솔하강(10% vs 40%,P<0.05);ACTH수평하강(5.92 ng/L±0.477 ng/L vs 6.79 ng/L±0.651 ng/L,P<0.05). 결론:재만성응격조건하,대서출현장도균군실조、장도통투성승고、신경내분비처우응격상태적현상,쌍기간균능구완해만성응격소도치적상술현상.