CAJ | 학술논문

目的探讨速度向量成像(VVI)技术结合潘生丁负荷试验是否可以早期检测糖尿病(DM)大鼠左室壁潜在的心肌功能障碍.方法雄性SD大鼠18只,腹腔注射链脲菌素复制DM模型,另12只体质量匹配的雄性SD大鼠腹腔注射等量柠檬酸缓冲液作为对照.分别在静息状态和潘生丁负荷后,对两组大鼠(12周)乳头肌水平左室短轴观行VVI及M型超声心动图检查,分别测量潘生丁负荷前后乳头肌水平左室短轴观各个节段心肌最大收缩速度(Vs)、切向应变(εc)、径向应变(εr)、收缩期最大切向应变率(SRc)、径向应变率(SRr)及室壁增厚率的平均值.处死动物,摘除心脏,行HE染色及透射电镜检查.结果静息状态下,DM组SRc较对照组显著减低(P＜0.05),其余指标与对照组的差异无统计学意义.潘生丁负荷后,DM组各指标均较对照组显著减低(P＜0.05).而M型检测的两组间室壁增厚率的差异并无统计学意义(P＞0.05).结论 VVI与负荷超声心动图相结合,可以更敏感地检测出DM大鼠潜在的室壁运动异常.SRc可能是更为敏感的指标,其在静息状态下便可以检测出这种室壁运动的异常.
목적 탐토속도향량성상(VVI)기술결합반생정부하시험시부가이조기검측당뇨병(DM)대서좌실벽잠재적심기공능장애.방법 웅성SD대서18지,복강주사련뇨균소복제DM모형,령12지체질량필배적웅성SD대서복강주사등량저몽산완충액작위대조.분별재정식상태화반생정부하후,대량조대서(12주)유두기수평좌실단축관행VVI급M형초성심동도검사,분별측량반생정부하전후유두기수평좌실단축관각개절단심기최대수축속도(Vs)、절향응변(εc)、경향응변(εr)、수축기최대절향응변솔(SRc)、경향응변솔(SRr)급실벽증후솔적평균치.처사동물,적제심장,행HE염색급투사전경검사.결과 정식상태하,DM조SRc교대조조현저감저(P＜0.05),기여지표여대조조적차이무통계학의의.반생정부하후,DM조각지표균교대조조현저감저(P＜0.05).이M형검측적량조간실벽증후솔적차이병무통계학의의(P＞0.05).결론 VVI여부하초성심동도상결합,가이경민감지검측출DM대서잠재적실벽운동이상.SRc가능시경위민감적지표,기재정식상태하편가이검측출저충실벽운동적이상.
Objective To investigate whether velocity vector imaging (VVI) combined with stress echocardiography could detect potential myocardial impairment of the left ventricle(LV) in diabetic rats.Methods DM rats ( n = 18,administered by STZ at 65 mg/kg) and control rats( n = 12) were performed with VVI and M-mode echocardiography both at rest and after dipyridamole stress 12 weeks later. Twodimensional echocardiographic cine loops and M-mode images of three consecutive beats were obtained from the short-axis views at the mid-LV level. The means of segmental peak systolic velocity(Vs), circumferential strain(εc) ,radial strain(εr), systolic circumferential and radial strain rate (SRc, SRr) and the percent wall thickening (WT% ,derived from M-mode) were obtained. After echocardiograms were performed,the hearts were excised and prepared for HE staining and ultrastructural observations under electron microscopy.Results At rest,only SRc in the DM group was significantly lower than that in the control group( P ＜0.05), the other parameters were statistically comparable between the two groups. After dipyridamole stress,all VVI values in the DM group were significantly lower than those in the control group ( P ＜0.05).However,there was no significant difference in WT% between the two groups either at rest or after dipyridamole stress ( P ＞0.05). Conclusions The VVI parameters combined with dipyridamole stress are more effective in evaluating potential myocardial impairment of the LV walls in diabetic rats. SRc might be more sensitive indices that can be used to detect myocardial impairment at rest.