中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
7期
887-890
,共4页
怀旭%沈嵩%石宁%刘国印%顿桓桓%许欣
懷旭%瀋嵩%石寧%劉國印%頓桓桓%許訢
부욱%침숭%석저%류국인%돈환환%허흔
模拟失重%分泌性免疫球蛋白A%分泌率
模擬失重%分泌性免疫毬蛋白A%分泌率
모의실중%분비성면역구단백A%분비솔
Simulated weightlessness%Secretory immunoglobulin A%Secretion rate
目的 探讨模拟失重对人体唾液分泌型免疫球蛋白A(SIgA)分泌率的影响.方法 14名男性被试者以头低位倾斜(-6°)模拟失重30d,地面正常男性14名作为对照,分别在实验前3、3、15、30d及后恢复第3天对受试者采集10 min唾液,并进行SIgA含量的测定并计算出SIgA分泌率.结果 试验组在地面模拟失重条件第3天唾液流量为[(7.1±0.8)ml/10 min],较受试前[(8.5±1.0)ml/10 min]及对照组的流量[(7.7±1.3)ml/10 min]有明显减少(P＜0.01),受试第15天唾液流量为(8.0±0.7)ml/10 min,也较受试前及对照组明显减少(P＜0.05);试验组受试者在受试的第3、15、30天口腔唾液SIgA浓度分别为(122±8j) ng/ml、(192±183)ng/ml和(168±183)ng/ml,SIgA的分泌率分别为(83±54) ng/min、( 14g±137) ng/min和( 135±144) ng/min,这两项指标均较受试前及对照组有明显增高(P＜0.01或P＜0.05),并以受试第3天的指标增高最为明显(P＜0.01).恢复期第3天的SIgA浓度(129±151) ng/ml及分泌率(105±124) ng/min与受试前及对照组相似(P＞0.05).对照组各时间点唾液分泌SIgA各项指标差异均无统计学意义.结论 在30d的地面模拟失重环境测试中,受试者唾液流量表现出先下降再恢复的特点,唾液SIgA的浓度和分泌率明显增加,提示口腔局部黏膜免疫水平增强,受试结束后各指标恢复到受试前水平.
目的 探討模擬失重對人體唾液分泌型免疫毬蛋白A(SIgA)分泌率的影響.方法 14名男性被試者以頭低位傾斜(-6°)模擬失重30d,地麵正常男性14名作為對照,分彆在實驗前3、3、15、30d及後恢複第3天對受試者採集10 min唾液,併進行SIgA含量的測定併計算齣SIgA分泌率.結果 試驗組在地麵模擬失重條件第3天唾液流量為[(7.1±0.8)ml/10 min],較受試前[(8.5±1.0)ml/10 min]及對照組的流量[(7.7±1.3)ml/10 min]有明顯減少(P＜0.01),受試第15天唾液流量為(8.0±0.7)ml/10 min,也較受試前及對照組明顯減少(P＜0.05);試驗組受試者在受試的第3、15、30天口腔唾液SIgA濃度分彆為(122±8j) ng/ml、(192±183)ng/ml和(168±183)ng/ml,SIgA的分泌率分彆為(83±54) ng/min、( 14g±137) ng/min和( 135±144) ng/min,這兩項指標均較受試前及對照組有明顯增高(P＜0.01或P＜0.05),併以受試第3天的指標增高最為明顯(P＜0.01).恢複期第3天的SIgA濃度(129±151) ng/ml及分泌率(105±124) ng/min與受試前及對照組相似(P＞0.05).對照組各時間點唾液分泌SIgA各項指標差異均無統計學意義.結論 在30d的地麵模擬失重環境測試中,受試者唾液流量錶現齣先下降再恢複的特點,唾液SIgA的濃度和分泌率明顯增加,提示口腔跼部黏膜免疫水平增彊,受試結束後各指標恢複到受試前水平.
목적 탐토모의실중대인체타액분비형면역구단백A(SIgA)분비솔적영향.방법 14명남성피시자이두저위경사(-6°)모의실중30d,지면정상남성14명작위대조,분별재실험전3、3、15、30d급후회복제3천대수시자채집10 min타액,병진행SIgA함량적측정병계산출SIgA분비솔.결과 시험조재지면모의실중조건제3천타액류량위[(7.1±0.8)ml/10 min],교수시전[(8.5±1.0)ml/10 min]급대조조적류량[(7.7±1.3)ml/10 min]유명현감소(P＜0.01),수시제15천타액류량위(8.0±0.7)ml/10 min,야교수시전급대조조명현감소(P＜0.05);시험조수시자재수시적제3、15、30천구강타액SIgA농도분별위(122±8j) ng/ml、(192±183)ng/ml화(168±183)ng/ml,SIgA적분비솔분별위(83±54) ng/min、( 14g±137) ng/min화( 135±144) ng/min,저량항지표균교수시전급대조조유명현증고(P＜0.01혹P＜0.05),병이수시제3천적지표증고최위명현(P＜0.01).회복기제3천적SIgA농도(129±151) ng/ml급분비솔(105±124) ng/min여수시전급대조조상사(P＞0.05).대조조각시간점타액분비SIgA각항지표차이균무통계학의의.결론 재30d적지면모의실중배경측시중,수시자타액류량표현출선하강재회복적특점,타액SIgA적농도화분비솔명현증가,제시구강국부점막면역수평증강,수시결속후각지표회복도수시전수평.
Objective To observe the secretion rate changes of salivary secretory immunoglobulin A(sIgA) during simulated weightlessness.Methods Fourteen healthy males were recruited and exposed to a-6°head down tilt(HDT) for 30 days for simulating a status of weighdessness.Another comparable group of 14 healthy males was selected as a blank control.Rest salivary specimens were collected at the time points of 3 days before HDT,Day 3,Day 15,Day 30 and 3 days post-weightlessness recovery period.The salivary volumes and SIgA concentrations of each participate were examined and the salivary flow rates and SIgA secretary rates were then calculated,respectively.Results Level of salivary volumes at HDT Day3(7.1±0.8ml/10min) and Day 15(8.0±0.7)ml/10min were significantly lower comparing to those of pr-HDT and blank control group(7.7±1.3) ml/10min(P ＜ 0.01;P ＜0.05).The concentrations of SIgA at HDT Day 3,Day 15 and Day 30 were(122±81) ng/ml,(192±183) ng/ml(168±183)ng/ml respectively;and the secretary rates of SIgA among H DT Day 3,Day 15 and Day 30 were(83±54) ng/min,(148±137) ng/min and(135±144) ng/min respectively.The concentrations and secretary rates of SIgA at HDT Day 3,Day 15 and Day 30 were significantly higher than that of pre-HDT and blank controls(P ＜0.01,P ＜0.05).There were no differences of SIgA concentrations at 3 days post-HDT,pre-HDT and blank controis.Conclusion During a period of 30 days of simulating weightlessness,the salivary flow rates at HDT Day 3and Day 15 are significantly lower than those of pre-HDT and controls.The salivary secretary rates of SIgA at HDT Day 3,Day 15 and Day 30 are significantly higher compared to normal.It is estimated that there is an elevated level of local mucosal immunity during artificial simulation of weightlessness on earth.
