中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2008年
6期
463-466
,共4页
袁文常%刘慧姝%郝荣增%熊正方
袁文常%劉慧姝%郝榮增%熊正方
원문상%류혜주%학영증%웅정방
胎盘%胎膜%滋养层%Toll样受体2%模式识别受体%先天性免疫
胎盤%胎膜%滋養層%Toll樣受體2%模式識彆受體%先天性免疫
태반%태막%자양층%Toll양수체2%모식식별수체%선천성면역
Placenta%Fetal membranes%Trophoblasts%Toll like receptor 2%Pattern recognition receptor%Innate immunity
目的 检测正常胎盘和胎膜组织中Toll样受体2(TLR2)的表达.方法 收集5例足月剖宫分娩胎盘和胎膜样本,运用RT-PCR法检测胎盘和胎膜组织中TLR2 mRNA的表达,运用免疫组织化学和Westen blot印迹方法检测TLR2蛋白质在胎盘和胎膜组织中的表达.结果 RT-PCR显示在mRNA水平,胎盘和胎膜组织中均有TLR2表达;Westen blot印迹显示TLR2抗体在胎盘和胎膜组织中相对分子质量为50000左右的位置有一条明显的条带;免疫组化研究显示TLR2在胎盘的合体滋养细胞、胎膜的羊膜上皮细胞及平滑绒毛膜滋养细胞中有表达.结论 TLR2在人胎盘和胎膜组织中的表达,提示其在妊娠期先天性免疫中可能发挥重要作用.
目的 檢測正常胎盤和胎膜組織中Toll樣受體2(TLR2)的錶達.方法 收集5例足月剖宮分娩胎盤和胎膜樣本,運用RT-PCR法檢測胎盤和胎膜組織中TLR2 mRNA的錶達,運用免疫組織化學和Westen blot印跡方法檢測TLR2蛋白質在胎盤和胎膜組織中的錶達.結果 RT-PCR顯示在mRNA水平,胎盤和胎膜組織中均有TLR2錶達;Westen blot印跡顯示TLR2抗體在胎盤和胎膜組織中相對分子質量為50000左右的位置有一條明顯的條帶;免疫組化研究顯示TLR2在胎盤的閤體滋養細胞、胎膜的羊膜上皮細胞及平滑絨毛膜滋養細胞中有錶達.結論 TLR2在人胎盤和胎膜組織中的錶達,提示其在妊娠期先天性免疫中可能髮揮重要作用.
목적 검측정상태반화태막조직중Toll양수체2(TLR2)적표체.방법 수집5례족월부궁분면태반화태막양본,운용RT-PCR법검측태반화태막조직중TLR2 mRNA적표체,운용면역조직화학화Westen blot인적방법검측TLR2단백질재태반화태막조직중적표체.결과 RT-PCR현시재mRNA수평,태반화태막조직중균유TLR2표체;Westen blot인적현시TLR2항체재태반화태막조직중상대분자질량위50000좌우적위치유일조명현적조대;면역조화연구현시TLR2재태반적합체자양세포、태막적양막상피세포급평활융모막자양세포중유표체.결론 TLR2재인태반화태막조직중적표체,제시기재임신기선천성면역중가능발휘중요작용.
Objective To detect Toll like receptor 2(TLR2) expression in tissues of normal human placenta and fetal membranes.Method Placenta and fetal membranes(5 cases)were collected from women with intact membranes underwent elective cesarean sections at term.Expression and localization of TLR2 was detected by RT-PCR,Westen blot analysis and immol/Lunohistochemistry.Results TLR2 messenger RNA was found in tissues of placenta and fetal membranes.Westen blot analysis with TLR2 antibody also yielded positive results for placenta and fetal membranes with one band site around 50 000.Immol/Lunohistochemical localization of TLR2 showed it to be present at syncytotrophoblast of placenta,cytotrophoblast of chorion and epithelial cell of amnion.Conclusion TLR2 expression in tissues of placenta and fetal membranes suggest that TLR2 may play an important role in the innate immol/Lunity of placenta.
