中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2010年
1期
44-46
,共3页
高压氧%缺氧缺血性脑损伤%颗粒酶b%凋亡
高壓氧%缺氧缺血性腦損傷%顆粒酶b%凋亡
고압양%결양결혈성뇌손상%과립매b%조망
Hyperbaric oxygen%Hypoxia-ischemia brain damage%Granzyme-b%Apoptosis
目的 研究新生大鼠缺氧缺血性脑损伤(HIBD)后脑组织颗粒酶b的表达及高压氧(HBO)的治疗作用.方法 110只7 d龄SD大鼠随机分为假手术组(n=10)、HIBD组(n=50)及HBO治疗组(n=50).采用经典的Rice法制作缺氧缺血性脑损伤模型.应用免疫组织化学法检测颗粒酶b表达变化及应用Epics XL流式细胞仪检测伤侧脑组织细胞凋亡情况.结果 缺氧缺血后6 h,HIBD组和HBO组伤侧脑组织颗粒酶b表达水平和细胞凋亡率即明显升高,于1 d达到峰值后开始下降,至7 d仍明显高于假手术组(P<0.05).HBO组伤侧大脑中颗粒酶b表达水平及细胞凋亡率在缺氧缺血后12 h和1、3、7 d均明显低于HIBD组(P<0.05).结论 新生大鼠HIBD后颗粒酶b表达增加;HBO可抑制新生大鼠HIBD后颗粒酶b的表达,减少缺氧缺血后神经元凋亡的发生,发挥脑保护作用.
目的 研究新生大鼠缺氧缺血性腦損傷(HIBD)後腦組織顆粒酶b的錶達及高壓氧(HBO)的治療作用.方法 110隻7 d齡SD大鼠隨機分為假手術組(n=10)、HIBD組(n=50)及HBO治療組(n=50).採用經典的Rice法製作缺氧缺血性腦損傷模型.應用免疫組織化學法檢測顆粒酶b錶達變化及應用Epics XL流式細胞儀檢測傷側腦組織細胞凋亡情況.結果 缺氧缺血後6 h,HIBD組和HBO組傷側腦組織顆粒酶b錶達水平和細胞凋亡率即明顯升高,于1 d達到峰值後開始下降,至7 d仍明顯高于假手術組(P<0.05).HBO組傷側大腦中顆粒酶b錶達水平及細胞凋亡率在缺氧缺血後12 h和1、3、7 d均明顯低于HIBD組(P<0.05).結論 新生大鼠HIBD後顆粒酶b錶達增加;HBO可抑製新生大鼠HIBD後顆粒酶b的錶達,減少缺氧缺血後神經元凋亡的髮生,髮揮腦保護作用.
목적 연구신생대서결양결혈성뇌손상(HIBD)후뇌조직과립매b적표체급고압양(HBO)적치료작용.방법 110지7 d령SD대서수궤분위가수술조(n=10)、HIBD조(n=50)급HBO치료조(n=50).채용경전적Rice법제작결양결혈성뇌손상모형.응용면역조직화학법검측과립매b표체변화급응용Epics XL류식세포의검측상측뇌조직세포조망정황.결과 결양결혈후6 h,HIBD조화HBO조상측뇌조직과립매b표체수평화세포조망솔즉명현승고,우1 d체도봉치후개시하강,지7 d잉명현고우가수술조(P<0.05).HBO조상측대뇌중과립매b표체수평급세포조망솔재결양결혈후12 h화1、3、7 d균명현저우HIBD조(P<0.05).결론 신생대서HIBD후과립매b표체증가;HBO가억제신생대서HIBD후과립매b적표체,감소결양결혈후신경원조망적발생,발휘뇌보호작용.
Objective To investigate effects of hyperbaric oxygen (HBO) on the expression of granzyme-b after hypoxia-ischemia brain damage (HIBD) in neonatal rats. Methods One hundred and ten rats aged 7 days were randomly divided into the sham group (n = 10), the HIBD group (n =50) and the HBO group (n =50). The HIBD model was developed by the Rice method. Samples were obtained following injury, and changes in the expression of granzyme-b were measured by immunohistochemical assay and apoptosis of injured brain cells were detected with Epics XL flow cytometer. Results Six hours after hypoxia - ischemia, the expression of granzyme-b and cell apoptosis rate in the injured brain tissue in the HIBD and HBO groups increased markedly, peaked 1d after hypoxia ischemia (HI), then, gradually decreased. However, it was still significantly higher than those of the sham group 7days after HI (P <0.05). The expression of granzyme-b in the damaged brain and apoptosis rate 12 h, 1 d, 3 d and 7 d after HI for the HBO group were all significantly lower than those of the HIBD group (P < 0.05). Conclusions Expression of granzyme-b in the brain tissue was up-regulated following HIBD. HBO might be effective in the protection of brain damage induced by HI through inhibiting the expression of granzyme-b and apoptosis of neurons.
