中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2010年
5期
381-383,后插6
,共4页
秦金保%沈尊理%祝加学%王晓盼%李康安%金羽青
秦金保%瀋尊理%祝加學%王曉盼%李康安%金羽青
진금보%침존리%축가학%왕효반%리강안%금우청
许旺细胞%超顺磁性纳米铁(SPIO)%体外示踪%磁共振成像
許旺細胞%超順磁性納米鐵(SPIO)%體外示蹤%磁共振成像
허왕세포%초순자성납미철(SPIO)%체외시종%자공진성상
Schwann cells%Superparamagnetic iron oxide (SPIO)%Labeling in vitro%MRI
目的 研究超顺磁性纳米铁颗粒(SPIO)体外标记小鼠许旺细胞(SCs)及MRI成像示踪的可行性.方法 分离、纯化新生C57BL/6小鼠(5～7 d)的许旺细胞,取已分别用25.0 μg/ml、50.0 μg/ml浓度SPIO标记的0.5×106、1.0×106、5.0×106个许旺细胞,普鲁士蓝染色和透射电镜观察标记细胞内铁颗粒的分布情况,并用不同MRI扫描序列,测定标记的细胞群信号.结果 0.5×106、1.0×106、5.0×106小鼠许旺细胞与不同浓度的SPIO共同培养24 h后,普鲁士蓝染色见细胞内有许多蓝染的铁颗粒;透射电镜检查显示致密的铁颗粒位于细胞的吞噬泡或溶酶体中.体外MRI呈明显的低信号改变,以T2WI和GRE/30°序列改变最为明显.结论 SPIO可以标记小鼠许旺细胞,应用MRI可以对其进行体外示踪和监测.
目的 研究超順磁性納米鐵顆粒(SPIO)體外標記小鼠許旺細胞(SCs)及MRI成像示蹤的可行性.方法 分離、純化新生C57BL/6小鼠(5～7 d)的許旺細胞,取已分彆用25.0 μg/ml、50.0 μg/ml濃度SPIO標記的0.5×106、1.0×106、5.0×106箇許旺細胞,普魯士藍染色和透射電鏡觀察標記細胞內鐵顆粒的分佈情況,併用不同MRI掃描序列,測定標記的細胞群信號.結果 0.5×106、1.0×106、5.0×106小鼠許旺細胞與不同濃度的SPIO共同培養24 h後,普魯士藍染色見細胞內有許多藍染的鐵顆粒;透射電鏡檢查顯示緻密的鐵顆粒位于細胞的吞噬泡或溶酶體中.體外MRI呈明顯的低信號改變,以T2WI和GRE/30°序列改變最為明顯.結論 SPIO可以標記小鼠許旺細胞,應用MRI可以對其進行體外示蹤和鑑測.
목적 연구초순자성납미철과립(SPIO)체외표기소서허왕세포(SCs)급MRI성상시종적가행성.방법 분리、순화신생C57BL/6소서(5～7 d)적허왕세포,취이분별용25.0 μg/ml、50.0 μg/ml농도SPIO표기적0.5×106、1.0×106、5.0×106개허왕세포,보로사람염색화투사전경관찰표기세포내철과립적분포정황,병용불동MRI소묘서렬,측정표기적세포군신호.결과 0.5×106、1.0×106、5.0×106소서허왕세포여불동농도적SPIO공동배양24 h후,보로사람염색견세포내유허다람염적철과립;투사전경검사현시치밀적철과립위우세포적탄서포혹용매체중.체외MRI정명현적저신호개변,이T2WI화GRE/30°서렬개변최위명현.결론 SPIO가이표기소서허왕세포,응용MRI가이대기진행체외시종화감측.
Objective To investigate the effects of labeling Schwann cells with different concentrations of SPIO, and to investigate the feasibility of in vitro MR imaging. Methods The C57BL/6 mices'Schwann cells were isolated, purified, and then 0.5 × 106, 1.0 × 106, 5.0 × 106 cells were labeled with 25.0 μg/ml, 50.0 μg/ml SPIO. Prussian blue stain and transmission electron microscope (TEM) were performed for showing intracellular iron. The signal intensity of cells were evaluated by 3.0 MRI with different sequences in vitro. Results Different cell population (0.5 × 106, 1.0 × 106,5.0 × 106) were cultured with different concentration SPIO about 24 hours. Dyeing degree of labeling cells stained by Prussion blue gradually deepened from 25.0 μg/ml to 50.0 μg/ml. Transmission electron microscope indicated that iron particles accumulated inendosomes/lysosomes. The MR signal intensity of labeling cells were inversely correlated with the concentration of SPIO groups in T2WI and GRE/30° imaging in vitro. Conclusion Schwann cells could be labeled effectively with SPIO, and MRI could be used to monitor these labeled cells in vitro.
