CAJ | 학술논문

目的检测静脉移植术后不同时期血管平滑肌细胞(VSMCSs)增殖与凋亡,进一步探讨其在血管内膜增生(IH)发生中的作用.方法60只Wistar大鼠建立自体静脉移植模型,自身正常静脉做对照,应用DNA原位缺口末端标记技术(TUNEL)及免疫组织化学方法检测不同时期(术后1、2、4、6、8周)凋亡及Ki-67表达情况,并作相关分析.结果术后1～8周,移植静脉VSMCS的TUNEL及Ki-67的阳性表达均显著高于对照组(P＜0.01).术后1、2周,二者表达均达高峰,且TUNEL表达低于Ki-67表达;而术后4～8周,TUNEL表达高于Ki-67表达.二者的表达具有显著相关性(r=0.813,P＜0.05).结论静脉移植术后VSMCSs的增殖与凋亡共存,且具有明显相关性,提示二者均参与静脉的重塑过程;术后移植静脉的再狭窄可能与VSMCS的凋亡和增殖失衡有关;对再狭窄的防治应采用调节VSMCS增殖和凋亡平衡的联合策略.
목적검측정맥이식술후불동시기혈관평활기세포(VSMCSs)증식여조망,진일보탐토기재혈관내막증생(IH)발생중적작용.방법60지Wistar대서건립자체정맥이식모형,자신정상정맥주대조,응용DNA원위결구말단표기기술(TUNEL)급면역조직화학방법검측불동시기(술후1、2、4、6、8주)조망급Ki-67표체정황,병작상관분석.결과술후1～8주,이식정맥VSMCS적TUNEL급Ki-67적양성표체균현저고우대조조(P＜0.01).술후1、2주,이자표체균체고봉,차TUNEL표체저우Ki-67표체;이술후4～8주,TUNEL표체고우Ki-67표체.이자적표체구유현저상관성(r=0.813,P＜0.05).결론정맥이식술후VSMCSs적증식여조망공존,차구유명현상관성,제시이자균삼여정맥적중소과정;술후이식정맥적재협착가능여VSMCS적조망화증식실형유관;대재협착적방치응채용조절VSMCS증식화조망평형적연합책략.
[Objective] To detect the functions of proliferation and apoptosis in the intimal hyperplasia of autogenous grafted veins through investigating vascular smooth muscle cells (VSMCS) in different phases after transplantation. [Methods] Autogenous vein graft model was established in 60 Wistar rats. The grafted veins were harvested from 1st to 8th weeks respectively after transplantation. The level of apoptosis and the expression of Ki-67were detected by means of TUNEL technique and immunohistochemical stain respectively in different stages after grafting. The autogenous normal vein served as auto-control. [Result] From 1st to 8th weeks after operation, the expressions of both TUNEL and Ki-67 were significantly higher than those of control groups (P ＜0.01). Both TUNEL and Ki-67 showed peak expression value in the first tow weeks, and the percentage of positive cells of TUNEL was lower than that of Ki-67. But a reversed result was shown from 4th to 8th weeks. The expressions of TUNEL and Ki-67 were significantly correlative (r =0.813, P ＜0.05). [Conclusions] After autogenous vein graft, both proliferation and apoptosis of VSMCSs coexisted and had a significant correlation, which indicated that the two processes participated in vascular remodeling. The unbalance of proliferation and apoptosis may relate to the stenosis of the vein graft, and regulating the balance of them may be useful to prevent restenosis.