南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2009年
8期
1582-1584
,共3页
李莉%梁刘萍%后桂荣%罗仁
李莉%樑劉萍%後桂榮%囉仁
리리%량류평%후계영%라인
加味玉屏风颗粒%变应性接触性皮炎%NF-κB
加味玉屏風顆粒%變應性接觸性皮炎%NF-κB
가미옥병풍과립%변응성접촉성피염%NF-κB
allergic contact dermatitis%modified Yupingfeng granule%nuclear factor-κB
目的 探讨中药加味玉屏风颗粒对变应性接触性皮炎NF-κB表达的影响.方法 建立小鼠变应性接触性皮炎模型,分组给药,采用免疫组化技术对各组局部皮肤NF-κB的表达水平进行检测.结果 治疗组小鼠在NF-κB染色强度、染色面积及表达阳性率等方面与生理盐水组均存在显著性差异(P<0.05).结论 加味玉屏风颗粒可有效抑制NF-κB的表达相关.这可能是其抗变态反应的作用机制之一.
目的 探討中藥加味玉屏風顆粒對變應性接觸性皮炎NF-κB錶達的影響.方法 建立小鼠變應性接觸性皮炎模型,分組給藥,採用免疫組化技術對各組跼部皮膚NF-κB的錶達水平進行檢測.結果 治療組小鼠在NF-κB染色彊度、染色麵積及錶達暘性率等方麵與生理鹽水組均存在顯著性差異(P<0.05).結論 加味玉屏風顆粒可有效抑製NF-κB的錶達相關.這可能是其抗變態反應的作用機製之一.
목적 탐토중약가미옥병풍과립대변응성접촉성피염NF-κB표체적영향.방법 건립소서변응성접촉성피염모형,분조급약,채용면역조화기술대각조국부피부NF-κB적표체수평진행검측.결과 치료조소서재NF-κB염색강도、염색면적급표체양성솔등방면여생리염수조균존재현저성차이(P<0.05).결론 가미옥병풍과립가유효억제NF-κB적표체상관.저가능시기항변태반응적작용궤제지일.
Objective To investigate the effect of modified Yupingfeng granule on nuclear factor-κB (NF-κB)expression in mice with allergic contact dermatitis (ACD). Methods Mouse models of ACD were treated with the granules at low, medium and high doses, with normal saline and hydrocortisone as the negative and positive controls, respectively. The expressions of NF-κB and its distribution in the lesions were detected using immunohistochemistry. Results The staining intensity, area and positive expression rates of NF-κB p50 were significantly different between the treatment group and the normal saline group (P<0.05). Conclusion Modified Yupingfeng granule can effectively inhibit the expression of NF-κB in ACD, which might be a possible mechanism for its therapeutic effect on ACD.
