CAJ | 학술논문

目的研究高能聚焦超声(HIFU)治疗后的兔肝VX2种植瘤氢质子磁共振波谱(~1H-MRS)的谱图特点及变化规律.方法建立20只新西兰大白兔肝VX2肿瘤模型,对其行HIFU治疗,分别于治疗前、后行常规MR平扫和二维多体素~1H-MRS检查,每只兔均选取瘤体中央区、周边区及瘤周正常肝组织作为感兴趣区(ROI),比较HIFU治疗前、后相同ROI的Cho/Cr及Lip/Cr.结果 20只兔肝VX2模型治疗前、后共获得28瘤次可纳入统计学分析的~1H-MRS图.典型的~1H-MRS图上可见6个主要的波峰,包括脂质(Lip)、谷氨酰胺和谷氨酸复合物(Glx)、胆碱(Cho)、乳酸(Lac)和肌酸(Cr).治疗后肿瘤中央区、周边区内Cho峰和Lac峰均较治疗前升高,Lip峰治疗后较治疗前降低;瘤周正常肝组织治疗前、后各主要代谢物变化不明显.治疗前后肿瘤中央区及周边区Cho/Cr、Lip/Cr值差异有统计学意义(P＜0.05).结论二维多体素~1H-MRS能反映VX2种植瘤经HIFU治疗后不同ROI主要代谢物水平变化.
목적 연구고능취초초성(HIFU)치료후적토간VX2충식류경질자자공진파보(~1H-MRS)적보도특점급변화규률.방법 건립20지신서란대백토간VX2종류모형,대기행HIFU치료,분별우치료전、후행상규MR평소화이유다체소~1H-MRS검사,매지토균선취류체중앙구、주변구급류주정상간조직작위감흥취구(ROI),비교HIFU치료전、후상동ROI적Cho/Cr급Lip/Cr.결과 20지토간VX2모형치료전、후공획득28류차가납입통계학분석적~1H-MRS도.전형적~1H-MRS도상가견6개주요적파봉,포괄지질(Lip)、곡안선알화곡안산복합물(Glx)、담감(Cho)、유산(Lac)화기산(Cr).치료후종류중앙구、주변구내Cho봉화Lac봉균교치료전승고,Lip봉치료후교치료전강저;류주정상간조직치료전、후각주요대사물변화불명현.치료전후종류중앙구급주변구Cho/Cr、Lip/Cr치차이유통계학의의(P＜0.05).결론 이유다체소~1H-MRS능반영VX2충식류경HIFU치료후불동ROI주요대사물수평변화.
Objective To observe imaging characteristics and changes of vivo two-dimension multi-voxel proton magnetic resonance spectroscopy (~1H-MRS) in the rabbit models of VX2 hepatic carcinoma after high intensity focused ultrasound (HIFU) treatment. Methods VX2 hepatic carcinoma models were established in 20 New Zealand rabbits. Routine MR and 2-dimentional ~1H-MRS scanning were performed before and after HIFU treatment. The central regions of interest (ROI) of the VX2 tumor, tumor border and paratumor normal liver tissues were selected. The Cho/Cr and Lip/Cr of the same ROI before and after HIFU treatment were compared. Results Total 28 satisfied spectrogram diagram of ~1H-MRS were brought into statistical analysis. Of all the spectra, 6 metabolite peaks were detected as lipids (Lip), glutamine and glutamate complex (Glx), choline (Cho), lactate (Lac) and creatine (Cr). Cho and Lac peak in tumor center and tumor border regions after HIFU treatment were higher than those before HIFU treatment. Lip peak was lower than before, and major metabolites of paratumor normal liver tissues did not changed significantly. Statistical differences of Cho/Cr and Lipid/Cr of tumor center and border region were found between before and after HIFU treatment (P＜0.05). Conclusion Two-dimension multi-voxel ~1H-MRS can reflect major changes in the level of metabolites of different ROI for hepatic VX2 carcinoma after HIFU treatment.