CAJ | 학술논문

由于难溶性矿物磷酸盐和有机磷的大量存在,土壤总磷虽高,但多数土壤缺乏有效磷,这是世界范围内农业生产的主要限制因素之一.土壤中有机磷的存在形式主要是植酸,为了提高土壤有效磷供给,利用根癌农杆菌菌株LBA4404,通过两步再生方法将植物表达载体pBINPR-phyI中含有的带胞外分泌信号肽序列的植酸酶基因转入油菜品种中双6号中,并获得转基因油菜56株,转化效率0.16%～9.20%.分子检测和酶活性检验表明,植酸酶基因已导入中双6号,转基因植株能有效表达植酸酶基因并向根外分泌植酸酶,转基因植株能以植酸为唯一磷源正常生长,非转基因植株则不能.结果表明,转基因植株根系分泌大量高活性植酸酶有助于土壤中有机磷释放出有效磷供植物利用;利用基因工程技术提高植物对土壤闭蓄态有机磷利用效率的前景是可期待的.
유우난용성광물린산염화유궤린적대량존재,토양총린수고,단다수토양결핍유효린,저시세계범위내농업생산적주요한제인소지일.토양중유궤린적존재형식주요시식산,위료제고토양유효린공급,이용근암농간균균주LBA4404,통과량보재생방법장식물표체재체pBINPR-phyI중함유적대포외분비신호태서렬적식산매기인전입유채품충중쌍6호중,병획득전기인유채56주,전화효솔0.16%～9.20%.분자검측화매활성검험표명,식산매기인이도입중쌍6호,전기인식주능유효표체식산매기인병향근외분비식산매,전기인식주능이식산위유일린원정상생장,비전기인식주칙불능.결과표명,전기인식주근계분비대량고활성식산매유조우토양중유궤린석방출유효린공식물이용;이용기인공정기술제고식물대토양폐축태유궤린이용효솔적전경시가기대적.
Phosphorus (P) deficiency in soil is a major constraint in agricultural production worldwide. Most soils contain signifi-cant amounts of total soil P that occurs in insoluble inorganic and organic fractious, but lack available phosphorus. Phytic acid is the major storage form of organic phosphorus in soil. In this experiment, the phytase gene with the signal peptide sequence of extracellular secretion was introduced into Brassica napus cv. Zhongshuang 4 via Agrobacterium tumefaciens LBA4404. Fifty six plants of transgenic Brassica napus were obtained and checked by PCR and phytase activity, most of them gave the positive re-sults. The transformation efficiency was 0.16-9.20%. When grown in MS medium with phytic acid as a sole phosphorus source under sterile conditions, transgenic Brassica napus plants were able to obtain inorganic phosphate from phytic acid and grew normally, but the wild-type plants not. These results show that extracellular phytase secreted from plant roots is a significant fac-tor in the utilization of phosphorus from phytate and indicate that there exists a prospect for using gene technology to improve the ability of plants to utilize accumulated forms of soil organic phosphorus.