CAJ | 학술논문

目的通过头孢西丁纸片法、耐甲氧西林金黄色葡萄球菌(MRSA)产色筛选平板法和聚合酶链反应(PCR)检测MRSA,评估MRSA产色筛选平板的敏感性和特异性.方法收集仁济医院2008年8月至9月临床分离的金黄色葡萄球菌68株,分别采用头孢西丁纸片法、MRSA产色筛选平板法和PCR检测MRSA,以PCR检测femA基因和mecA基因结果为金标准,比较MRSA产色筛选平板的敏感性和特异性.结果甲氧西林敏感金黄色葡萄球菌(MSSA)除万古霉素、替考拉宁和利奈唑胺外,对其他11种抗菌药物的敏感率明显高于MRSA.68株金黄色葡萄球菌中,头孢西丁纸片法筛选出 50株MRSA,产色平板法筛选出51株MRSA,PCR检测到51株mecA基因阳性,MRSA产色筛选平板结果和mecA基因检测结果符合率为100%.结论 MRSA产色筛选平板可用于临床快速检测MRSA.
목적 통과두포서정지편법、내갑양서림금황색포도구균(MRSA)산색사선평판법화취합매련반응(PCR)검측MRSA,평고MRSA산색사선평판적민감성화특이성.방법 수집인제의원2008년8월지9월림상분리적금황색포도구균68주,분별채용두포서정지편법、MRSA산색사선평판법화PCR검측MRSA,이PCR검측femA기인화mecA기인결과위금표준,비교MRSA산색사선평판적민감성화특이성.결과 갑양서림민감금황색포도구균(MSSA)제만고매소、체고랍저화리내서알외,대기타11충항균약물적민감솔명현고우MRSA.68주금황색포도구균중,두포서정지편법사선출 50주MRSA,산색평판법사선출51주MRSA,PCR검측도51주mecA기인양성,MRSA산색사선평판결과화mecA기인검측결과부합솔위100%.결론 MRSA산색사선평판가용우림상쾌속검측MRSA.
Objective To evaluate the sensitivity and specificity of methicillin-resistant Staphylococcus aureus (MRSA) chromogenic agar medium by cefoxitin disk test and polymerase chain reaction (PCR) amplification of MRSA genes. Methods 68 Staphylococcus aureus strains from clinical isolates were collected from August to September 2008 in Renji hospital. The sensitivity and specificity of the MRSA chromogenic agar medium were compared with PCR standard method. MRSA were determined by cefoxitin disk test, MRSA chromogenic agar medium and PCR amplification. Results The sensitivity of 11 antibiotics from methicillin-susceptible Staphylococcus aureus (MSSA) strains was significantly higher than that from MRSA strains except the other three tested antibiotics such as vancomycin, teicoplanin and linezolid. In the 68 tested Staphylococcus aureus strains,50 strains were positive from MRSA strains by cefoxitin disk test,while 51 strains were positive for MRSA strains by chromogenic agar medium test and 51 strains were positive for mecA gene by PCR amplification. The results of MRSA chromogenic agar medium test were the same as those of PCR amplification for mecA gene. Conclusions MRSA chromogenic agar medium test can be used in fast detection of clinical MRSA strains.