生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2010年
2期
178-183
,共6页
李祖明%张洪勋%薛文通%张保国%李鸿玉%张晓岚
李祖明%張洪勛%薛文通%張保國%李鴻玉%張曉嵐
리조명%장홍훈%설문통%장보국%리홍옥%장효람
碱性内切聚半乳糖醛酸酶%筛选%固态发酵%提取条件优化%鉴定
堿性內切聚半乳糖醛痠酶%篩選%固態髮酵%提取條件優化%鑒定
감성내절취반유당철산매%사선%고태발효%제취조건우화%감정
Alkaline endopolygalacturonase%Screening Solid-state fermentation Optimization of extraction condition%Identi fication
从碱性土样中经分离筛选得到1株固态发酵产碱性内切聚半乳糖醛酸酶活力较高的菌株.经提酶条件优化得到较优的酶液提取提条件为30倍Na_2CO_3/NaHCO_3缓冲液提取1 h.从形态特征、培养特征、生理生化特征以及分子生物学鉴定结果来看,该菌株属B.gibsonii.
從堿性土樣中經分離篩選得到1株固態髮酵產堿性內切聚半乳糖醛痠酶活力較高的菌株.經提酶條件優化得到較優的酶液提取提條件為30倍Na_2CO_3/NaHCO_3緩遲液提取1 h.從形態特徵、培養特徵、生理生化特徵以及分子生物學鑒定結果來看,該菌株屬B.gibsonii.
종감성토양중경분리사선득도1주고태발효산감성내절취반유당철산매활력교고적균주.경제매조건우화득도교우적매액제취제조건위30배Na_2CO_3/NaHCO_3완충액제취1 h.종형태특정、배양특정、생리생화특정이급분자생물학감정결과래간,해균주속B.gibsonii.
A bacterial strain highly producing alkaline endopolygalacturonase by solid-state fermentation was obtained from a sampie of alkaline soil.The recovery ratio of the enzyme from the isolate was the highest after 1 h of extraction in 30 volume of Na_2CO_3/NaHCO_3 buffer at 30℃.The isolate was confirmed to be a strain of B.gibsonii after identified by colony characteristics,morphological characteristics,physiologica and biochemical characteristics and molecular biology methods.
