中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2011年
7期
1155-1158
,共4页
宋恩%李云建%章玉冰%姚黎清%周如丹%李宏昆%李兴国%张春强%王兵%赵学凌
宋恩%李雲建%章玉冰%姚黎清%週如丹%李宏昆%李興國%張春彊%王兵%趙學凌
송은%리운건%장옥빙%요려청%주여단%리굉곤%리흥국%장춘강%왕병%조학릉
深静脉血栓形成%大鼠%动物模型%精氨酸酶Ⅰ%一氧化氮%一氧化氮合酶
深靜脈血栓形成%大鼠%動物模型%精氨痠酶Ⅰ%一氧化氮%一氧化氮閤酶
심정맥혈전형성%대서%동물모형%정안산매Ⅰ%일양화담%일양화담합매
背景:近期相关研究发现精氨酸酶Ⅰ与多种心血管疾病发生发展有关,但国内外相关研究大都集中在动脉,并未对静脉性疾病,进行广泛研究.目的:观察深静脉血栓形成模型大鼠精氨酸酶Ⅰ的表达变化,分析其在深静脉血栓形成过程中可能发挥的作用.方法:将100只SD大鼠随机分为正常对照组和模型组,采用血管钳夹股静脉+双后肢髋人字石膏外固定制动的方式建立大鼠创伤性深静脉血栓模型,根据不同的观察时间点和血栓形成的不同病理生理过程情况分为血栓形成前组、血栓形成高峰期组和无血栓形成组,提取各组血液RNA,应用实时荧光定量PCR检测精氨酸酶Ⅰ在各组血细胞中的表达变化.结果与结论:血栓形成高峰期组精氨酸酶Ⅰ表达量比其他3组明显上升(P < 0.01),正常对照组、血栓形成前组和无血栓形成组精氨酸酶Ⅰ表达差异无显著性意义 (P > 0.05).提示精氨酸酶Ⅰ与深静脉血栓形成密切相关.
揹景:近期相關研究髮現精氨痠酶Ⅰ與多種心血管疾病髮生髮展有關,但國內外相關研究大都集中在動脈,併未對靜脈性疾病,進行廣汎研究.目的:觀察深靜脈血栓形成模型大鼠精氨痠酶Ⅰ的錶達變化,分析其在深靜脈血栓形成過程中可能髮揮的作用.方法:將100隻SD大鼠隨機分為正常對照組和模型組,採用血管鉗夾股靜脈+雙後肢髖人字石膏外固定製動的方式建立大鼠創傷性深靜脈血栓模型,根據不同的觀察時間點和血栓形成的不同病理生理過程情況分為血栓形成前組、血栓形成高峰期組和無血栓形成組,提取各組血液RNA,應用實時熒光定量PCR檢測精氨痠酶Ⅰ在各組血細胞中的錶達變化.結果與結論:血栓形成高峰期組精氨痠酶Ⅰ錶達量比其他3組明顯上升(P < 0.01),正常對照組、血栓形成前組和無血栓形成組精氨痠酶Ⅰ錶達差異無顯著性意義 (P > 0.05).提示精氨痠酶Ⅰ與深靜脈血栓形成密切相關.
배경:근기상관연구발현정안산매Ⅰ여다충심혈관질병발생발전유관,단국내외상관연구대도집중재동맥,병미대정맥성질병,진행엄범연구.목적:관찰심정맥혈전형성모형대서정안산매Ⅰ적표체변화,분석기재심정맥혈전형성과정중가능발휘적작용.방법:장100지SD대서수궤분위정상대조조화모형조,채용혈관겸협고정맥+쌍후지관인자석고외고정제동적방식건립대서창상성심정맥혈전모형,근거불동적관찰시간점화혈전형성적불동병리생리과정정황분위혈전형성전조、혈전형성고봉기조화무혈전형성조,제취각조혈액RNA,응용실시형광정량PCR검측정안산매Ⅰ재각조혈세포중적표체변화.결과여결론:혈전형성고봉기조정안산매Ⅰ표체량비기타3조명현상승(P < 0.01),정상대조조、혈전형성전조화무혈전형성조정안산매Ⅰ표체차이무현저성의의 (P > 0.05).제시정안산매Ⅰ여심정맥혈전형성밀절상관.
BACKGROUND:Studies in recent years have demonstrated that arginase Ⅰ contribute to the process of numerous cardiovascular diseases,however,most of studies focus on arteries,few regarding venous diseases.OBJECTIVE:To explore the changes of arginase Ⅰ expression in rat traumatic deep venous thrombosis models,and to analyze the possible function of arginase Ⅰ in deep venous thrombosis formation.METHODS:Totally 100 Sprague Dawley rats were randomly divided into the control and model groups.Traumatic deep venous thrombosis models were established by clamping the femoral vein and immobilizing the bilateral hind limbs (hip spica cast fixation),and assigned into initial thrombosis,peak thrombosis and non-thrombosis groups according to different observing time points and pathophysiological situations of thrombosis.Whole blood RNA of each group was extracted,and the change of arginase I expression in blood cells of each group was detected by real-time PCR.RESULTS AND CONCLUSUON:Expression of arginase Ⅰ in the peak thrombosis group was significantly increased compared with other 3 groups (P < 0.01).There were no significances among control,initial thrombosis and non-thrombosis groups (P > 0.05).The finding demonstrated that arginase Ⅰ is closely related to deep vein thrombosis formation.
