国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2009年
6期
341-343
,共3页
激光扫描共聚焦显微镜%结肠腺癌%lovo细胞%转移
激光掃描共聚焦顯微鏡%結腸腺癌%lovo細胞%轉移
격광소묘공취초현미경%결장선암%lovo세포%전이
Laser scanning confocal microscopy%Metastatic colon cancer%Lovo cells%Metastasis
目的 应用激光扫描共聚焦显微镜体外观察结肠癌细胞的定向转移,探讨一种激光扫描共聚焦显微镜观察肿瘤细胞体外转移的方法.方法 以肝转移性低分化结肠腺癌lovo细胞株为模型,采用激光扫描共聚焦显微镜结合Transwell小室模型在体外观察了lovo细胞在趋化因子白细胞介素8作用下的侵袭和迁移的特征.结果 在白细胞介素8作用下lovo细胞侵袭能力增强,呈现出阿米巴样的变形迁移方式.提示在激光扫描共聚焦显微镜下观察到的结肠腺癌lovo细胞的转移呈现一种类白细胞趋化的特征.结论 激光扫描共聚焦显微镜是研究肿瘤细胞转移的一个新的研究工具.
目的 應用激光掃描共聚焦顯微鏡體外觀察結腸癌細胞的定嚮轉移,探討一種激光掃描共聚焦顯微鏡觀察腫瘤細胞體外轉移的方法.方法 以肝轉移性低分化結腸腺癌lovo細胞株為模型,採用激光掃描共聚焦顯微鏡結閤Transwell小室模型在體外觀察瞭lovo細胞在趨化因子白細胞介素8作用下的侵襲和遷移的特徵.結果 在白細胞介素8作用下lovo細胞侵襲能力增彊,呈現齣阿米巴樣的變形遷移方式.提示在激光掃描共聚焦顯微鏡下觀察到的結腸腺癌lovo細胞的轉移呈現一種類白細胞趨化的特徵.結論 激光掃描共聚焦顯微鏡是研究腫瘤細胞轉移的一箇新的研究工具.
목적 응용격광소묘공취초현미경체외관찰결장암세포적정향전이,탐토일충격광소묘공취초현미경관찰종류세포체외전이적방법.방법 이간전이성저분화결장선암lovo세포주위모형,채용격광소묘공취초현미경결합Transwell소실모형재체외관찰료lovo세포재추화인자백세포개소8작용하적침습화천이적특정.결과 재백세포개소8작용하lovo세포침습능력증강,정현출아미파양적변형천이방식.제시재격광소묘공취초현미경하관찰도적결장선암lovo세포적전이정현일충류백세포추화적특정.결론 격광소묘공취초현미경시연구종류세포전이적일개신적연구공구.
Objective To explore the feasibility of applying laser scanning confocal microscopy to investigate the metastatic characteristic of tumor cells in vitro. Methods The confocal microscope combined with transwell assay was employed to observe the invasion and migration of the metastatic colon cancer ]ovo cells when stimulated by IL-8. Results The results show that the number of invasive lovo cells in the stimulated group was much higher than the unstimulated group (P<0.05). The migration way of lovo cells observed under the confocal microscopy was ameba like movement. These findings suggest that the metastatic ability of lovo cells stimulated by IL-8 was enhanced. Conclusion The metastatic characteristic of colon adenocarcinoma lovo cells is similar to the chemotactic movement of leukocytes.
