中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
6期
745-747
,共3页
不育,女(雌)性/治疗%肝炎,乙型/治疗%授精,人工
不育,女(雌)性/治療%肝炎,乙型/治療%授精,人工
불육,녀(자)성/치료%간염,을형/치료%수정,인공
Infertility,female/TH%Hepatitis B/TH%Insemination,artificial
目的 探讨不孕症乙肝病毒携带者接受体外受精治疗的安全性.方法 对149例女性不孕症乙肝病毒携带者及144例男性乙肝病毒携带者采用酶联免疫方法(ELISA),于取卵日分别检测患者的血液、卵泡液、受精培养液、精液、洗涤后精液的HBV血清学五项标志物的阳性率,按HBV血清学五项标志物将男女HBV携带者分为HBV大三阳和小三阳组,分析其卵泡液及受精培养液;精液和洗涤后精液HBV的阳性率.结果 18例女性血液HBV大三阳者,卵泡液、受精培养液中检测HBV大三阳均为100.0%;131例女性血液HBV小三阳者,其中,64.1%(84/131)的卵泡液及6.1%(8/131)受精培养液为小三阳;23例男性血液HBV大三阳者精液及洗涤后HBV检测大三阳阳性率为26.1%(6/23),洗涤后精液HBV检测均为阴性;121例男性血液HBV小三阳者,精液HBV检测小三阳阳性率为5.8%(7/121),洗涤后精液HBV检测均为阴性.结论 体外受精处理不能改变女性乙肝大三阳患者的通过卵泡液及受精培养液传播HBV的风险,可降低女性乙肝小三阳患者和男性HBV患者的传播风险.
目的 探討不孕癥乙肝病毒攜帶者接受體外受精治療的安全性.方法 對149例女性不孕癥乙肝病毒攜帶者及144例男性乙肝病毒攜帶者採用酶聯免疫方法(ELISA),于取卵日分彆檢測患者的血液、卵泡液、受精培養液、精液、洗滌後精液的HBV血清學五項標誌物的暘性率,按HBV血清學五項標誌物將男女HBV攜帶者分為HBV大三暘和小三暘組,分析其卵泡液及受精培養液;精液和洗滌後精液HBV的暘性率.結果 18例女性血液HBV大三暘者,卵泡液、受精培養液中檢測HBV大三暘均為100.0%;131例女性血液HBV小三暘者,其中,64.1%(84/131)的卵泡液及6.1%(8/131)受精培養液為小三暘;23例男性血液HBV大三暘者精液及洗滌後HBV檢測大三暘暘性率為26.1%(6/23),洗滌後精液HBV檢測均為陰性;121例男性血液HBV小三暘者,精液HBV檢測小三暘暘性率為5.8%(7/121),洗滌後精液HBV檢測均為陰性.結論 體外受精處理不能改變女性乙肝大三暘患者的通過卵泡液及受精培養液傳播HBV的風險,可降低女性乙肝小三暘患者和男性HBV患者的傳播風險.
목적 탐토불잉증을간병독휴대자접수체외수정치료적안전성.방법 대149례녀성불잉증을간병독휴대자급144례남성을간병독휴대자채용매련면역방법(ELISA),우취란일분별검측환자적혈액、란포액、수정배양액、정액、세조후정액적HBV혈청학오항표지물적양성솔,안HBV혈청학오항표지물장남녀HBV휴대자분위HBV대삼양화소삼양조,분석기란포액급수정배양액;정액화세조후정액HBV적양성솔.결과 18례녀성혈액HBV대삼양자,란포액、수정배양액중검측HBV대삼양균위100.0%;131례녀성혈액HBV소삼양자,기중,64.1%(84/131)적란포액급6.1%(8/131)수정배양액위소삼양;23례남성혈액HBV대삼양자정액급세조후HBV검측대삼양양성솔위26.1%(6/23),세조후정액HBV검측균위음성;121례남성혈액HBV소삼양자,정액HBV검측소삼양양성솔위5.8%(7/121),세조후정액HBV검측균위음성.결론 체외수정처리불능개변녀성을간대삼양환자적통과란포액급수정배양액전파HBV적풍험,가강저녀성을간소삼양환자화남성HBV환자적전파풍험.
Objective To study the security in HBV carried infertility patients during the in-vitro-fertilization procedure. Methods Serologic testing of HBV infection (HBsAg, HBsAb, HBeAg, anti-HBe, anti-HBc-IgG) of blood, follicle fluid/sperm and fertilization culture medium/post-washing sperm in female/male carried patients were detected by ELISA on the day of oocytes collection. Results In 18 female patients who were seropositive for HBsAg, HBeAg and anti-HBc-lgG, the same antigen and antibody could be detected in all 18 folli-cle-fluid and fertilization culture medium(100%, 100%). In 131 female patients who were sernpesitive for HBsAg, anti-HBe, anti-HBc-IgG, the same antigen and antibody could be detected in 84 follicle-fluid(64. 1%), and 8 fertilization culture medium(6. 1%). If the patients'follicle-fluld was negative for all the markers, their fertilization culture medium remained negative. In 23 male patients who were sero-positive for HBsAg , HBeAg,and anti-HBc-lgG, the same antigen and antibody could be detected in only 6 sperm (26. 1%), all the postwashing sperm were exhibited negative. In 121 nude patients who were seropositive for HBsAg, anti-HBe, anti-HBc-lgG, the same antigen and antibody could be detected in only 7 sperm (5. 8%), and all the post-washing sperm were negative. Conclusion In IVF-ET procedures, the risk of HBV transmission by follicle-fluid and fertilization culture medium in those female patients who showed serepesitive for HBsAg , HBeAg, and anti-HBc-lgG can not be decreased. The risk in those female patients who showed seropositive for HBsAg, anti-HBe, anti-HBe-lgG and male patients in IVF-ET procedures can be decreased.
