中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2012年
5期
305-308
,共4页
李敏%梁翼%吴晓惠%蔚浩%余文景%杨南萍%黄向阳
李敏%樑翼%吳曉惠%蔚浩%餘文景%楊南萍%黃嚮暘
리민%량익%오효혜%위호%여문경%양남평%황향양
红斑狼疮,系统性%肝脏损害%γ-谷氨酰转移酶%碱性磷酸酶%转氨酶%干扰素诱导蛋白4%蛋白激酶
紅斑狼瘡,繫統性%肝髒損害%γ-穀氨酰轉移酶%堿性燐痠酶%轉氨酶%榦擾素誘導蛋白4%蛋白激酶
홍반랑창,계통성%간장손해%γ-곡안선전이매%감성린산매%전안매%간우소유도단백4%단백격매
Lupus erythematosus,systemic%Liver deteriment%γ-glutamyl transpeptidase%Alkaline phosphatase%Transaminase%IFIT4%PRKR
目的 比较干扰素诱导蛋白4(IFIT4)、蛋白激酶(PRKR)在不同类型狼疮肝损害患者的表达差异,及不同类型狼疮肝损害的临床表现及免疫学指标,分析总结其各自特点.方法 将符合狼疮性肝损害诊断的62例患者分为单纯肝酶升高组、单纯胆酶升高组和肝酶胆酶均升高组,再选取同期62例无狼疮性肝损害患者作对照,收集其相关临床数据及外周血白细胞IFIT4、PRKR基因表达进行比较分析.数据采用x2检验、方差分析及Pearson或Spearman相关分析.结果 ①胆酶升高组易并发皮肤黏膜损害(x2=5.625,P=0.018)、狼疮肾炎(x2=5.631,P=0.019),出现贫血及血小板减少(99+21,P=0.028;81±45,P=0.004)等血液系统损害,其C反应蛋白(CRP) (33±43.P=0.004),nRNP (x2=4.862,P=0.027)和抗SSA抗体(=8.087,P=0.004)阳性率均明显高于其他组;②系统性红斑狼疮性肝损害组抗核糖体蛋白(Rib)抗体阳性率明显高于无SLE性肝损害组(x2=19.542,P=0.000);③IFIT4在不同组中表达差异无统计学意义,蛋白激酶在单纯胆酶升高组中明显高表达(F=3.54,P=0.018),并与γ-俗氨酰转氨酶(γ-GGT)、碱性磷酸酶呈正相关(r=0.34,P<0.05;r=0.3.P<0.05).结论 不同类型肝损害有着不同的临床和实验室特点,蛋白激酶在单纯胆酶升高组明显高表达.
目的 比較榦擾素誘導蛋白4(IFIT4)、蛋白激酶(PRKR)在不同類型狼瘡肝損害患者的錶達差異,及不同類型狼瘡肝損害的臨床錶現及免疫學指標,分析總結其各自特點.方法 將符閤狼瘡性肝損害診斷的62例患者分為單純肝酶升高組、單純膽酶升高組和肝酶膽酶均升高組,再選取同期62例無狼瘡性肝損害患者作對照,收集其相關臨床數據及外週血白細胞IFIT4、PRKR基因錶達進行比較分析.數據採用x2檢驗、方差分析及Pearson或Spearman相關分析.結果 ①膽酶升高組易併髮皮膚黏膜損害(x2=5.625,P=0.018)、狼瘡腎炎(x2=5.631,P=0.019),齣現貧血及血小闆減少(99+21,P=0.028;81±45,P=0.004)等血液繫統損害,其C反應蛋白(CRP) (33±43.P=0.004),nRNP (x2=4.862,P=0.027)和抗SSA抗體(=8.087,P=0.004)暘性率均明顯高于其他組;②繫統性紅斑狼瘡性肝損害組抗覈糖體蛋白(Rib)抗體暘性率明顯高于無SLE性肝損害組(x2=19.542,P=0.000);③IFIT4在不同組中錶達差異無統計學意義,蛋白激酶在單純膽酶升高組中明顯高錶達(F=3.54,P=0.018),併與γ-俗氨酰轉氨酶(γ-GGT)、堿性燐痠酶呈正相關(r=0.34,P<0.05;r=0.3.P<0.05).結論 不同類型肝損害有著不同的臨床和實驗室特點,蛋白激酶在單純膽酶升高組明顯高錶達.
목적 비교간우소유도단백4(IFIT4)、단백격매(PRKR)재불동류형랑창간손해환자적표체차이,급불동류형랑창간손해적림상표현급면역학지표,분석총결기각자특점.방법 장부합랑창성간손해진단적62례환자분위단순간매승고조、단순담매승고조화간매담매균승고조,재선취동기62례무랑창성간손해환자작대조,수집기상관림상수거급외주혈백세포IFIT4、PRKR기인표체진행비교분석.수거채용x2검험、방차분석급Pearson혹Spearman상관분석.결과 ①담매승고조역병발피부점막손해(x2=5.625,P=0.018)、랑창신염(x2=5.631,P=0.019),출현빈혈급혈소판감소(99+21,P=0.028;81±45,P=0.004)등혈액계통손해,기C반응단백(CRP) (33±43.P=0.004),nRNP (x2=4.862,P=0.027)화항SSA항체(=8.087,P=0.004)양성솔균명현고우기타조;②계통성홍반랑창성간손해조항핵당체단백(Rib)항체양성솔명현고우무SLE성간손해조(x2=19.542,P=0.000);③IFIT4재불동조중표체차이무통계학의의,단백격매재단순담매승고조중명현고표체(F=3.54,P=0.018),병여γ-속안선전안매(γ-GGT)、감성린산매정정상관(r=0.34,P<0.05;r=0.3.P<0.05).결론 불동류형간손해유착불동적림상화실험실특점,단백격매재단순담매승고조명현고표체.
Objective To analyze the expression of IFIT4,PRKR and investigate the clinical and immunology features of different types of liver involvement in patients with systemic lupus erythematosus (SLE).Methods Clinical data of 62 cases of SLE with liver damage and 62 cases of SLE without liver damage were collected.Peripheral venous blood samples were obtained and total RNA were extracted and transcribed into cDNA.Sybr green dye based real-time quantitative PCR method was used to compare the expression levels of IFIT4,PRKR in patients with SLE.Clinical parameters were analyzed by ANOVA,Chi-square test,Pearson's or Spearman's test.Results ① The increase of γ-GT or ALP was correlated with rash and oral ulcer (x2=5.625,P=0.018),lupus nephritis (x2=5.631,P=0.019),anemia,thrombocytopenia (99±21,P=0.028; 81±45,P=0.004,),CRP (33±43,P=0.004).The positive rate of nRNP (x2=4.862,P=0.027 ) and SSA (x2=8.087,P=0.004) was higher in patients with liver damage than other groups; ② The positive rate of anti-Rib antibody in SLE with liver damage was significantly higher than SLE without liver damage (x2=19.542,P=0.000); ③ There was no difference in the expression of IFIT4 among these groups,but higher expression of PRKR was detected in the group of patients with increased γ-glutamyl transpeptidase (γ-GT) or ALP(F=3.54,P=0.018).Conclusion The different types of liver damage in SLE patients have different clinical and immunology characteristics.The expression of PRKR is higher in patients with increased γ-GT or ALP.