中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2009年
7期
445-450
,共6页
熊佶%刘颖%李超%朱静静%叶诸榕%毛颖%汪寅
熊佶%劉穎%李超%硃靜靜%葉諸榕%毛穎%汪寅
웅길%류영%리초%주정정%협제용%모영%왕인
少突神经胶质瘤%染色体,人,1对%染色体,人,19对%杂合子丢失%肿瘤抑制蛋白质p53
少突神經膠質瘤%染色體,人,1對%染色體,人,19對%雜閤子丟失%腫瘤抑製蛋白質p53
소돌신경효질류%염색체,인,1대%염색체,인,19대%잡합자주실%종류억제단백질p53
Oligodendroglioma%Chromosomes,human,pairl%Chromosomes,human,pairl9%Loss of heterozagosity%Tumor suppressor protein p53
目的 探讨少突胶质细胞瘤染色体1p/19q杂合性缺失及p53蛋白的表达情况,与星形细胞起源的肿瘤进行比较研究并探讨其意义.方法 选择2004-2005年间,经病理组织学诊断为不同类型和级别的胶质瘤合计191例,包括:WHOⅡ级少突胶质细胞瘤116例,其中30例为新鲜组织;间变性少突胶质细胞瘤45例和不同级别星形细胞起源的肿瘤石蜡组织30例;采用PCR-微卫星技术检测染色体1p/19q杂合性缺失情况;采用免疫组织化学方法 对184例胶质瘤石蜡切片p53蛋白表达情况进行半定量分析.结果 86例WHO Ⅱ级少突胶质细胞瘤石蜡标本染色体1p缺失率为69.8%(60/86)、19q缺失率为64.0%(55/86)、1p/19q联合缺失率为57.0%(49/86);45例间变性少突胶质细胞瘤中,1p缺失率为71.1%(32/45)、19q缺失率为60.0%(27/45)、1p/19q联合缺失率为55.6%(25/45);两种级别间差异无统计学意义(P>0.05).30例WHO Ⅱ级少突胶质细胞瘤新鲜标本染色体1p缺失率为70.0%(21/30)、19q缺失率为63.3%(19/30)、1p/19q联合缺失率为60.0%(18/30),与石蜡标本的缺失率比较差异无统计学意义(P>0.05).30例星形细胞起源的肿瘤染色体对应三种缺失率分别为23.3%(7/30)、33.3%(10/30)及20.0%(6/30),与少突胶质细胞瘤差异有统计学意义(P<0.05).86例WHO Ⅱ级少突胶质细胞瘤中,仅7例有p53蛋白阳性表达(占8.1%);45例间变性少突胶质细胞瘤中,有14例呈阳性表达(31.1%),两者差异有统计学意义(P=0.007).少突胶质细胞瘤p53蛋白阳性表达明显低于星形细胞起源的肿瘤(P=0.001).在间变性少突胶质细胞瘤中,染色体1p/19q杂合性缺失与p53蛋白阳性表达呈负相关(P<0.05).结论 石蜡和新鲜组织均可用于染色体1p/19q杂合性缺失的检测.在间变性少突胶质细胞瘤中,染色体1p/19q杂合性缺失与p53蛋白阳性表达呈负相关.检测少突胶质细胞瘤染色体1p/19q杂合性缺失和p53蛋白表达,对提高病理诊断的精确性、指导治疗及预后判断具有重要意义.
目的 探討少突膠質細胞瘤染色體1p/19q雜閤性缺失及p53蛋白的錶達情況,與星形細胞起源的腫瘤進行比較研究併探討其意義.方法 選擇2004-2005年間,經病理組織學診斷為不同類型和級彆的膠質瘤閤計191例,包括:WHOⅡ級少突膠質細胞瘤116例,其中30例為新鮮組織;間變性少突膠質細胞瘤45例和不同級彆星形細胞起源的腫瘤石蠟組織30例;採用PCR-微衛星技術檢測染色體1p/19q雜閤性缺失情況;採用免疫組織化學方法 對184例膠質瘤石蠟切片p53蛋白錶達情況進行半定量分析.結果 86例WHO Ⅱ級少突膠質細胞瘤石蠟標本染色體1p缺失率為69.8%(60/86)、19q缺失率為64.0%(55/86)、1p/19q聯閤缺失率為57.0%(49/86);45例間變性少突膠質細胞瘤中,1p缺失率為71.1%(32/45)、19q缺失率為60.0%(27/45)、1p/19q聯閤缺失率為55.6%(25/45);兩種級彆間差異無統計學意義(P>0.05).30例WHO Ⅱ級少突膠質細胞瘤新鮮標本染色體1p缺失率為70.0%(21/30)、19q缺失率為63.3%(19/30)、1p/19q聯閤缺失率為60.0%(18/30),與石蠟標本的缺失率比較差異無統計學意義(P>0.05).30例星形細胞起源的腫瘤染色體對應三種缺失率分彆為23.3%(7/30)、33.3%(10/30)及20.0%(6/30),與少突膠質細胞瘤差異有統計學意義(P<0.05).86例WHO Ⅱ級少突膠質細胞瘤中,僅7例有p53蛋白暘性錶達(佔8.1%);45例間變性少突膠質細胞瘤中,有14例呈暘性錶達(31.1%),兩者差異有統計學意義(P=0.007).少突膠質細胞瘤p53蛋白暘性錶達明顯低于星形細胞起源的腫瘤(P=0.001).在間變性少突膠質細胞瘤中,染色體1p/19q雜閤性缺失與p53蛋白暘性錶達呈負相關(P<0.05).結論 石蠟和新鮮組織均可用于染色體1p/19q雜閤性缺失的檢測.在間變性少突膠質細胞瘤中,染色體1p/19q雜閤性缺失與p53蛋白暘性錶達呈負相關.檢測少突膠質細胞瘤染色體1p/19q雜閤性缺失和p53蛋白錶達,對提高病理診斷的精確性、指導治療及預後判斷具有重要意義.
목적 탐토소돌효질세포류염색체1p/19q잡합성결실급p53단백적표체정황,여성형세포기원적종류진행비교연구병탐토기의의.방법 선택2004-2005년간,경병리조직학진단위불동류형화급별적효질류합계191례,포괄:WHOⅡ급소돌효질세포류116례,기중30례위신선조직;간변성소돌효질세포류45례화불동급별성형세포기원적종류석사조직30례;채용PCR-미위성기술검측염색체1p/19q잡합성결실정황;채용면역조직화학방법 대184례효질류석사절편p53단백표체정황진행반정량분석.결과 86례WHO Ⅱ급소돌효질세포류석사표본염색체1p결실솔위69.8%(60/86)、19q결실솔위64.0%(55/86)、1p/19q연합결실솔위57.0%(49/86);45례간변성소돌효질세포류중,1p결실솔위71.1%(32/45)、19q결실솔위60.0%(27/45)、1p/19q연합결실솔위55.6%(25/45);량충급별간차이무통계학의의(P>0.05).30례WHO Ⅱ급소돌효질세포류신선표본염색체1p결실솔위70.0%(21/30)、19q결실솔위63.3%(19/30)、1p/19q연합결실솔위60.0%(18/30),여석사표본적결실솔비교차이무통계학의의(P>0.05).30례성형세포기원적종류염색체대응삼충결실솔분별위23.3%(7/30)、33.3%(10/30)급20.0%(6/30),여소돌효질세포류차이유통계학의의(P<0.05).86례WHO Ⅱ급소돌효질세포류중,부7례유p53단백양성표체(점8.1%);45례간변성소돌효질세포류중,유14례정양성표체(31.1%),량자차이유통계학의의(P=0.007).소돌효질세포류p53단백양성표체명현저우성형세포기원적종류(P=0.001).재간변성소돌효질세포류중,염색체1p/19q잡합성결실여p53단백양성표체정부상관(P<0.05).결론 석사화신선조직균가용우염색체1p/19q잡합성결실적검측.재간변성소돌효질세포류중,염색체1p/19q잡합성결실여p53단백양성표체정부상관.검측소돌효질세포류염색체1p/19q잡합성결실화p53단백표체,대제고병리진단적정학성、지도치료급예후판단구유중요의의.
Objective To study the status of loss of heterozygosity ( LOH) of chromosome lp/19q and p53 protein expression in oligodendroglioma,as compared to astrocytoma.Methods One hundred and ninety-one cases of glioma of different histologic types and grades,including 116 cases of low-grade of oligodendroglioma ( 86 paraffin-embedded and 30 fresh tissues) ,45 cases of anaplastic oligodendroglioma (all paraffin-embedded tissues) and 30 cases of astrocytoma of various grades ( all paraffin-embedded tissues),were enrolled into the study.The LOH of chromosome lp/19q was investigated by polymerase chain reaction ( PCR) -based microsatellite analysis.The p53 protein expression was demonstrated by immunohistochemical staining.Results The rates of lp loss, 19q loss and lp/19q loss were 69.8% ,64%,and 57.0% respectively in the 86 paraffin-embedded low-grade oligodendroglioma samples,as compared to 71.1%,60.0% and 55.6% respectively in the 45 paraffin-embedded anaplastic oligodendroglioma samples.There was no difference of LOH of lp/19q between low-grade oligodendroglioma and anaplastic oligodendroglioma (P > 0.05).In the 30 cases of low-grade oligodendroglioma with fresh tissues available,the rates of lp loss,19q loss and lp/19q loss were 70.0%,63.3% and 60.0% respectively.The LOH of lp/19q between paraffin-embedded and fresh samples was not statistically significant (P >0.05).In the 30 cases of astrocytoma,the rates of lp loss,19q loss and lp/19q loss were 23.3%,33.3% and 20.0% respectively,which were significantly less than those in oligodendroglioma (P < 0.05).The expression of p53 protein was significantly lower in low-grade oligodendroglioma (8.1 % ) than in anaplastic oligodendroglioma (31.1%,P = 0.007).The expression of p53 protein in oligodendroglioma was also lower than in astrocytoma ( P = 0.001 ).Furthermore,p53 protein expression negatively correlated with lp/19q loss in anaplastic oligodendroglioma (P <0.05).Conclusions Both paraffin-embedded and fresh tissues are suitable for analysis of LOH of chromosome lp/19q.Oligodendroglioma demonstrates a higher frequency of LOH of chromosome lp/19q and lower expression of p53 protein than astrocytoma.The LOH of chromosome lp/19q negatively correlates with the expression of p53 protein.These parameters have both diagnostic and prognostic values.
