CAJ | 학술논문

目的探讨去细胞异种神经(acellular xenogeneic nerve,AXN)复合骨髓基质干细胞(bone marrow stem cells,BMSCs)修复周围神经缺损的效果.方法体外培养大鼠BMSCs；取雌性Wistar大鼠60只,随机分为3组,每组20只,建立右坐骨神经10 mm缺损修复模型.A组:BMSCs与AXN复合修复神经缺损；B组:单纯AXN修复神经缺损；C组:自体神经移植组.术后4、12周依次进行干细胞的转归、移植免疫、神经电生理检测、新生神经组织学观察和小腿三头肌肌纤维横径等检测,判断坐骨神经功能恢复情况.结果术后移植物内均可见细胞生长,A、C组细胞数目较多,排列整齐,只有A组S-100免疫组化染色阳性细胞内可见BrdU阳性表达,术后12周再生神经已通过远端缝合口.A组、C组组织学及电生理检测指标均优于B组(P＜0.05),A组与C组差异无统计学意义(P＞0.05).结论 BMSCs作为种子细胞可以在体内存活,并可分化为SCs,其与AXN复合构建组织工程神经修复神经缺损的效果接近于自体神经移植.
목적 탐토거세포이충신경(acellular xenogeneic nerve,AXN)복합골수기질간세포(bone marrow stem cells,BMSCs)수복주위신경결손적효과.방법 체외배양대서BMSCs；취자성Wistar대서60지,수궤분위3조,매조20지,건립우좌골신경10 mm결손수복모형.A조:BMSCs여AXN복합수복신경결손；B조:단순AXN수복신경결손；C조:자체신경이식조.술후4、12주의차진행간세포적전귀、이식면역、신경전생리검측、신생신경조직학관찰화소퇴삼두기기섬유횡경등검측,판단좌골신경공능회복정황.결과 술후이식물내균가견세포생장,A、C조세포수목교다,배렬정제,지유A조S-100면역조화염색양성세포내가견BrdU양성표체,술후12주재생신경이통과원단봉합구.A조、C조조직학급전생리검측지표균우우B조(P＜0.05),A조여C조차이무통계학의의(P＞0.05).결론 BMSCs작위충자세포가이재체내존활,병가분화위SCs,기여AXN복합구건조직공정신경수복신경결손적효과접근우자체신경이식.
Objective To evaluate the outcomes of repairing peripheral nerve defects with acellular xenogeneic nerve (AXN) repopulated with bone marrow stromal cells (BMSCs). Methods Rat BMSCs were cultured in vitro.Right sciatic nerve 10 mm defect model was established in 60 female Wistar rats that were randomly divided into 3 groups,with 20 each.The gaps were repaired using one of the following:AXN repopulated with BMSCs (group A),AXN (group B) and autologous nerve graft (group C).Tissues were harvested 4 and 12 weeks after the operation.The status of BMSCs and axonal regeneration were evaluated by general observation,electrophysiological examination,gastrocnemius muscle fiber measurement,histological observation and scanning electron microscopy (SEM) observation of the regenerating nerve. Results After transplantation viable cells were observed in all the grafts.More cells were noted in group A and C,which were well oriented.BrdU positive expression was seen in S-100 positive cells only in group A.After 12 weeks,regenerating axons had passed the defect.Histological and electrophysiological testing were better in group A and C than in group B ( P ＜ 0.05 ).No significant difference was detected between group A and C ( P ＞ 0.05).Conclusion BMSCs can survive in the body,and can differentiate into SCs.AXN repopulated with BMSCs leads to comparable results to autologous nerve graft when used to repair nerve defects.