CAJ | 학술논문

目的观察联合美金刚和米诺环素用药在肌萎缩性脊髓侧索硬化病(ALS)转基因小鼠模型治疗中的协同作用.方法 ALS转基因小鼠被随机分成生理盐水对照组、米诺环素组、美金刚组和米诺环素/美金刚组.对腹腔注射米诺环素和美金刚后ALS小鼠的运动能力、发病时间、生存时间以及腓肠肌重量变化进行定量分析.结果 (1)与对照组发病时间(98.3±4.6)d比较,米诺环素组和美金刚组分别为(117.1 ±7.4)、(114.0±3.5) d(P ＜0.05)；对照组生存期为(129.4±6.2)d,米诺环素组和美金刚组分别为(146.2±5.9)、(141.9±6.3) d(P ＜0.05).联合治疗组发病时间和生存期分别为(126.2±8.4)d和(159.1±3.9)d.与对照组比较,米诺环素组、美金刚组和联合治疗组生存时间分别延长13％、11％和23％.(2)对照组小鼠第16周时腓肠肌重量为(83.5±10.2)mg,米诺环素组和美金刚组分别为(133.7 ±12.5)、(130.1 ±10.3) mg,联合治疗组为(186.3±17.9) mg,与对照组和单一用药组比较,差异有统计学意义(P＜0.05).结论联合米诺环素和美金刚对治疗ALS小鼠具有协同治疗作用.
목적 관찰연합미금강화미낙배소용약재기위축성척수측색경화병(ALS)전기인소서모형치료중적협동작용.방법 ALS전기인소서피수궤분성생리염수대조조、미낙배소조、미금강조화미낙배소/미금강조.대복강주사미낙배소화미금강후ALS소서적운동능력、발병시간、생존시간이급비장기중량변화진행정량분석.결과 (1)여대조조발병시간(98.3±4.6)d비교,미낙배소조화미금강조분별위(117.1 ±7.4)、(114.0±3.5) d(P ＜0.05)；대조조생존기위(129.4±6.2)d,미낙배소조화미금강조분별위(146.2±5.9)、(141.9±6.3) d(P ＜0.05).연합치료조발병시간화생존기분별위(126.2±8.4)d화(159.1±3.9)d.여대조조비교,미낙배소조、미금강조화연합치료조생존시간분별연장13％、11％화23％.(2)대조조소서제16주시비장기중량위(83.5±10.2)mg,미낙배소조화미금강조분별위(133.7 ±12.5)、(130.1 ±10.3) mg,연합치료조위(186.3±17.9) mg,여대조조화단일용약조비교,차이유통계학의의(P＜0.05).결론 연합미낙배소화미금강대치료ALS소서구유협동치료작용.
Objective To investigate whether combination of minocycline with memantine would result in increased therapeutic effects in animal model of amyotrophic lateral sclerosis (ALS).Methods ALS mice were divided randomly into normal saline control group,minocycline group,memantine group and minocycline/memantine group.Motor strength,coordination,onset and mortality of ALS mice were evaluated after minocycline and/or memantine treatment.The weight of gastrocnemius was quantitatively analyzed as well.Results ( 1 ) Disease onset was delayed to 117 and 114 days respectively in the minocycline- and memantine-treated groups as compared with 98 days in the control group (P ＜ 0.05).Survival was similarly delayed in the minocycline and memantine groups to 146 and 143 days respectively as compared with 129 days in the control group ( P ＜ 0.05 ).Disease onset and survival in minocycline-memantine combination group were delayed to 126 and 159 days.Survival was extended by 13％,11％ and 23％ in the minocycline,memantine and minocycline-memantine groups respectively as compared with the control group; (2) The weight of gastrocnemius in minocycline group and memantine group was increased to 133.7 mg and 130.1 mg respectively as compared with 83.5 mg in the control group (P ＜0.05).The weight of gastrocnemius in minocycline-memantine group was 186.3 mg,which was significantly greater than in the control group,minocycline group or menmatine group (P ＜ 0.05).Conclusion Combination of minocycline and memantine in ALS mice model results in synergistic therapeutic effects.