中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
23期
1630-1634
,共5页
吴东进%郝爱华%张程%崔福斋%王修文%高春正%周成军%刘军莉%谯勇%马胜忠
吳東進%郝愛華%張程%崔福齋%王脩文%高春正%週成軍%劉軍莉%譙勇%馬勝忠
오동진%학애화%장정%최복재%왕수문%고춘정%주성군%류군리%초용%마성충
修复外科手术%骨生成%组织疗法%干细胞
脩複外科手術%骨生成%組織療法%榦細胞
수복외과수술%골생성%조직요법%간세포
Reconstructive surgical procedures%Osteogenesis%Tissue therapy%Stem cells
目的 探讨内皮祖细胞(EPC)与纳米羟基磷灰石/胶原/聚乳酸(nHAC/PLA)复合物在修复兔桡骨节段性骨缺损中促进血管新生及新骨形成的作用.方法 从新西兰大兔骨髓分离、培养EPC并予以鉴定,将EPC与nHAC/PLA支架复合,以扫描电镜(SEM)观察EPC在支架内的生长状态.将36只新西兰大白兔随机数字表法分为3组,制作桡骨节段性骨缺损模型,分别植入EPC-nHAC/PLA复合物(A组,n=16)、nHAC/PLA支架材料(B组,n=16),其余4只(C组)不用任何内植物以作空白对照.分别于不同的时间点处死动物,收取桡骨并行放射学、组织学检查及血管密度计数.结果 自骨髓分离的细胞为EPC,SEM显示其与nHAC/PLA复合后黏附、生长、增殖良好.A组骨缺损处的放射学评分[(4.3±0.5)比(3.0±0.0),均P＜0.05]、新骨形成区占骨缺损区的百分比[(96.0％±4.3％)比(34.0％±6.6％),均P ＜0.05)]、微血管密度[(7.0±0.4)比(4.5±0.4),均P＜0.05)]均高于B组,差异有统计学意义,组织学显示A组的新骨形成及支架降解快于B组.C组无明显新骨形成.结论 EPC-nHAC/PLA复合物能明显促进骨缺损处血管新生和新骨形成,提示其在应用组织工程骨修复大段骨缺损的再血管化和成骨方面有潜在的应用价值.
目的 探討內皮祖細胞(EPC)與納米羥基燐灰石/膠原/聚乳痠(nHAC/PLA)複閤物在脩複兔橈骨節段性骨缺損中促進血管新生及新骨形成的作用.方法 從新西蘭大兔骨髓分離、培養EPC併予以鑒定,將EPC與nHAC/PLA支架複閤,以掃描電鏡(SEM)觀察EPC在支架內的生長狀態.將36隻新西蘭大白兔隨機數字錶法分為3組,製作橈骨節段性骨缺損模型,分彆植入EPC-nHAC/PLA複閤物(A組,n=16)、nHAC/PLA支架材料(B組,n=16),其餘4隻(C組)不用任何內植物以作空白對照.分彆于不同的時間點處死動物,收取橈骨併行放射學、組織學檢查及血管密度計數.結果 自骨髓分離的細胞為EPC,SEM顯示其與nHAC/PLA複閤後黏附、生長、增殖良好.A組骨缺損處的放射學評分[(4.3±0.5)比(3.0±0.0),均P＜0.05]、新骨形成區佔骨缺損區的百分比[(96.0％±4.3％)比(34.0％±6.6％),均P ＜0.05)]、微血管密度[(7.0±0.4)比(4.5±0.4),均P＜0.05)]均高于B組,差異有統計學意義,組織學顯示A組的新骨形成及支架降解快于B組.C組無明顯新骨形成.結論 EPC-nHAC/PLA複閤物能明顯促進骨缺損處血管新生和新骨形成,提示其在應用組織工程骨脩複大段骨缺損的再血管化和成骨方麵有潛在的應用價值.
목적 탐토내피조세포(EPC)여납미간기린회석/효원/취유산(nHAC/PLA)복합물재수복토뇨골절단성골결손중촉진혈관신생급신골형성적작용.방법 종신서란대토골수분리、배양EPC병여이감정,장EPC여nHAC/PLA지가복합,이소묘전경(SEM)관찰EPC재지가내적생장상태.장36지신서란대백토수궤수자표법분위3조,제작뇨골절단성골결손모형,분별식입EPC-nHAC/PLA복합물(A조,n=16)、nHAC/PLA지가재료(B조,n=16),기여4지(C조)불용임하내식물이작공백대조.분별우불동적시간점처사동물,수취뇨골병행방사학、조직학검사급혈관밀도계수.결과 자골수분리적세포위EPC,SEM현시기여nHAC/PLA복합후점부、생장、증식량호.A조골결손처적방사학평분[(4.3±0.5)비(3.0±0.0),균P＜0.05]、신골형성구점골결손구적백분비[(96.0％±4.3％)비(34.0％±6.6％),균P ＜0.05)]、미혈관밀도[(7.0±0.4)비(4.5±0.4),균P＜0.05)]균고우B조,차이유통계학의의,조직학현시A조적신골형성급지가강해쾌우B조.C조무명현신골형성.결론 EPC-nHAC/PLA복합물능명현촉진골결손처혈관신생화신골형성,제시기재응용조직공정골수복대단골결손적재혈관화화성골방면유잠재적응용개치.
Objective To explore the roles of nano-hydroxyapatite/collagen/PLA (nHAC/PLA)plus endothelial progenitor cells (EPCs) in repairing segmental bone defects of rabbit radius and enhancing angiogenesis and new bone formation.Methods EPCs isolated from New Zealand white rabbit bone marrow were cultured,identified and seeded into nHAC/PLA scaffolds.And the growth of EPCs in scaffolds was observed under scanning electron microscopy ( SEM ).Thirty-six were randomly divided into 3 groups to establish segmental bone defect models in radii.Two groups were implanted with EPCs/scaffolds constructs ( group A,n =16) and scaffolds alone (group B,n =16) respectively.The remaining four rabbits were used as negative control (group C) and nothing was implanted.Animals were sacrificed at different timepoints and radii harvested to undergo radiological examination,histological examination and microvessle density test.Results These cells isolated from bone marrow were confirmed as EPCs.SEM showed that EPCs attached to the nHAC/PLA scaffolds, grew and proliferated well.Animal experiments revealed that radiological scores (5w:2.25 ±0.50 vs 1.00 ±0.00; 10w:2.75 ±0.50 vs 1.75 ±0.50; 15w:4.25 ±0.50 vs 3.0 ± 0.0 ; each P ＜ 0.05 ),percentage of new bone formation area in bone defect regions (5w:29.0％ ± 3.5％ vs 8.1％ ±0.8％; 10w:63.4％ ±5.5％ vs 16.6％ ±1.3％; 15w:96.0％ ±4.3％ vs 34.0％ ±6.6％ ; each P ＜ 0.05 ) and microvessel density ( 2w:13.5 ± 0.9 vs 4.3 ± 1.0; 5w:9.8 ± 0.7 vs 4.8 ±0.3 ; 10w:7.0 ± 0.4 vs 4.5 ± 0.4; each P ＜ 0.05 ) in group A were significantly higher than those in group B.No new bone formation occurred in group C.Conclusion The composite structure of EPCs-nHAC/PLA can enhance angiogenesis and new bone formation in segmental bone defects in rabbit radii.It may become a potential candidate of promoting revascularization of tissue engineering bone and repairing large bone defects.
