中国癌症研究(英文版)
中國癌癥研究(英文版)
중국암증연구(영문판)
CHINESE JOURNAL OF CANCER RESEARCH
2004年
4期
260-264
,共5页
王伟%Marianne Ifversen%赵春亭%汪洪毅%赵洪国
王偉%Marianne Ifversen%趙春亭%汪洪毅%趙洪國
왕위%Marianne Ifversen%조춘정%왕홍의%조홍국
Neuroblastoma%Fluorescence in situ hybridization%Nmyc gene
Objective: To establish a method to improve the detection of disseminated tumor cells in bone marrow and peripheral blood samples of neuroblastoma patients and analysis of cytogenetic aberration. Methods: Immunofluorescent staining was performed using a cocktail of primary monoclonal neuroblastoma antibodies (14.G2a, 5.1H11). Fluorescence in situ hybridization was applied with fluorescent probes specific for Nmyc genes afterwards. A novel computer assisted scanning system for automatic search, image analysis and repositioning of these positive cells was developed. Fifty-six bone marrow and peripheral blood samples from 7 patients were evaluated by this method. Results: Fluorescence in situ hybridization can be combined with immunofluorescent staining in detecting Nmyc amplification in neuroblastoma patients. Fluorescence in situ hybridization results correlated well with data obtained by conventional cytogenetic procedures. Conclusion: The technique described allows search of tumor cells in the bone marrow as well as detection of Nmyc amplification in interphase nuclei.