夜来香提取物对小鼠的镇痛作用
야래향제취물대소서적진통작용
Analgesic effect of Cestrum nocturnum L. extract on mice
  • 万方数据
    中国临床康复 중국림상강복
    CHINESE JOURNAL OF CLINICAL REHABILITATION
    2006年 35期 172-174 ,共3页

    黄龙岗%张乡城%肖海%叶和杨%曾靖

    황룡강%장향성%초해%협화양%증정

    镇痛%月见草%植物提取物 鎮痛%月見草%植物提取物
    진통%월견초%식물제취물
    背景:夜来香水提取物具有抗心律失常、局部麻醉以及中枢抑制作用.目的:探讨夜来香提取物对小鼠的镇痛作用,为临床疼痛治疗寻找新药.设计:随机对照观察.单位:赣南医学院现代教育中心与药理教研室.材料:实验于2005-03/04在赣南医学院科研中心实验室完成.①选取健康成年昆明种小鼠150只用于以下4个独立实验.②药品:夜来香提取物由沈阳药科大学植化教研室提供(药品批号:2002080901);盐酸吗啡注射液(沈阳第一制药厂,批号000305);盐酸纳洛酮注射液[盐侨(湖南)制药有限公司,批号20021109].方法:①夜来香提取物对醋酸引起小鼠扭体反应的实验:小鼠40只,随机数字表法分为4组,10只/组,分别腹腔注射0.02 mL/g生理盐水,0.10,0.20 mg/g夜来香提取物,0.10 mg/g氨基比林.15 min后腹腔注射6g/L冰醋酸0.01 mL/g,观察记录15 min内各组小鼠扭体反应次数.②夜来香提取物对小鼠热板法致痛作用的实验:雌性小鼠40只,随机数字表法分为4组,10只/组,分别腹腔注射0.02 mL/g生理盐水,0.10,0.20 mg/g夜来香提取物,0.01 mg/g吗啡,用热板法测定给药后15,30,60 min的痛觉反应.③纳洛酮拮抗吗啡、夜来香提取物对小鼠热板法致痛作用的实验:雌性小鼠30只,随机数字表法分为3组,10只/组,分别腹腔注射0.02 mL/g生理盐水,0.004 mg/g纳洛酮+0.01 mg/g吗啡,0.004 mg/g纳洛酮+0.10 mg/g夜来香提取物,热板法测定给药后15,30,60 min的痛觉反应.④夜来香提取物对电刺激致痛的实验:小鼠40只,随机数字表法分为4组,10只/组,分别腹腔注射0.02 mL/g生理盐水,0.10,0.20 mg/g夜来香提取物,1 g/L吗啡,于给药后20,35,50,70 min重复电刺激,电刺激法测定痛觉反应.*主要观察指标:①小鼠扭体反应次数.②热板法致小鼠痛觉反应的时间.③电刺激法致小鼠镇痛率.结果:共选取健康成年昆明种小鼠150只用于4个独立实验,全部进入结果分析.①小鼠扭体反应次数:0.10,0.20 mg/g夜来香提取物及0.10 mg/g氨基比林对醋酸诱发小鼠扭体反应有非常显著的镇痛作用,给药后扭体反应次数均少于生理盐水组(20.2±10.8,14.5±7.6,7.6±4.5,50.6±15.5,P＜0.01),且夜来香提取物的镇痛效果呈剂量依赖性.②热板法致小鼠痛觉反应的时间:0.10,0.20 mg/g夜来香提取物对热板致痛有显著的镇痛作用,给药后15,30,60min痛觉反应的时间均长于生理盐水组(P＜0.05或0.01),且呈剂量依赖性.纳洛酮0.004 mg/g+夜来香提取物0.10 mg/g组给药后各时间点痛觉反应的时间均长于生理盐水组(P＜0.05或0.01),但纳洛酮0.004 mg/g+吗啡0.01 mg/g组与生理盐水组相接近.③电刺激法致小鼠镇痛率:夜来香提取物0.10,0.20 mg/g组及吗啡组给药后20,35,50,70 min镇痛率均高于生理盐水组P＜0.01).结论:夜来香提取物具有明显的镇痛作用,且镇痛强度呈剂量依赖性.其镇痛作用并非通过激动阿片受体而实现的.
    배경:야래향수제취물구유항심률실상、국부마취이급중추억제작용.목적:탐토야래향제취물대소서적진통작용,위림상동통치료심조신약.설계:수궤대조관찰.단위:공남의학원현대교육중심여약리교연실.재료:실험우2005-03/04재공남의학원과연중심실험실완성.①선취건강성년곤명충소서150지용우이하4개독립실험.②약품:야래향제취물유침양약과대학식화교연실제공(약품비호:2002080901);염산마배주사액(침양제일제약엄,비호000305);염산납락동주사액[염교(호남)제약유한공사,비호20021109].방법:①야래향제취물대작산인기소서뉴체반응적실험:소서40지,수궤수자표법분위4조,10지/조,분별복강주사0.02 mL/g생리염수,0.10,0.20 mg/g야래향제취물,0.10 mg/g안기비림.15 min후복강주사6g/L빙작산0.01 mL/g,관찰기록15 min내각조소서뉴체반응차수.②야래향제취물대소서열판법치통작용적실험:자성소서40지,수궤수자표법분위4조,10지/조,분별복강주사0.02 mL/g생리염수,0.10,0.20 mg/g야래향제취물,0.01 mg/g마배,용열판법측정급약후15,30,60 min적통각반응.③납락동길항마배、야래향제취물대소서열판법치통작용적실험:자성소서30지,수궤수자표법분위3조,10지/조,분별복강주사0.02 mL/g생리염수,0.004 mg/g납락동+0.01 mg/g마배,0.004 mg/g납락동+0.10 mg/g야래향제취물,열판법측정급약후15,30,60 min적통각반응.④야래향제취물대전자격치통적실험:소서40지,수궤수자표법분위4조,10지/조,분별복강주사0.02 mL/g생리염수,0.10,0.20 mg/g야래향제취물,1 g/L마배,우급약후20,35,50,70 min중복전자격,전자격법측정통각반응.*주요관찰지표:①소서뉴체반응차수.②열판법치소서통각반응적시간.③전자격법치소서진통솔.결과:공선취건강성년곤명충소서150지용우4개독립실험,전부진입결과분석.①소서뉴체반응차수:0.10,0.20 mg/g야래향제취물급0.10 mg/g안기비림대작산유발소서뉴체반응유비상현저적진통작용,급약후뉴체반응차수균소우생리염수조(20.2±10.8,14.5±7.6,7.6±4.5,50.6±15.5,P＜0.01),차야래향제취물적진통효과정제량의뢰성.②열판법치소서통각반응적시간:0.10,0.20 mg/g야래향제취물대열판치통유현저적진통작용,급약후15,30,60min통각반응적시간균장우생리염수조(P＜0.05혹0.01),차정제량의뢰성.납락동0.004 mg/g+야래향제취물0.10 mg/g조급약후각시간점통각반응적시간균장우생리염수조(P＜0.05혹0.01),단납락동0.004 mg/g+마배0.01 mg/g조여생리염수조상접근.③전자격법치소서진통솔:야래향제취물0.10,0.20 mg/g조급마배조급약후20,35,50,70 min진통솔균고우생리염수조P＜0.01).결론:야래향제취물구유명현적진통작용,차진통강도정제량의뢰성.기진통작용병비통과격동아편수체이실현적.
    BACKGROUND: It has been considered that Cestrum nocturnum L. (CNL) has the effects of antiarrhythmia, local anesthesia and central inhibition.OBJECTIVE: To investigate the analgesic effect of CNL extract on mice,so as to find new drugs for clinical treatment of pain.DESIGN: A randomized control observation.SETTING: Center of Modern Education and Department of Pharmacology,Gannan Medical College.MATERIALS: The experiments were carried out in the laboratory of scientific research center, Gannan Medical College between March and April in 2005. ① A total of 150 healthy adult Kunming mice were used in four independent experiments. ② Drugs: CNL extract was provided by the Department of Phytochemistry, Shenyang Pharmaceutical University (batch number: 2002080901), morphine hydrochloride injection by Shenyang No.1Pharmaceutical Factory (batch number: 000305), and naloxone hydrochloride injection by Yanqiao (Hunan) Pharmaceutical Co. Ltd., (batch number:20021109).METHODS: ① Effects of CNL extract on writhing times induced by acetic acid: Forty female mice were randomly divided into four groups with 10 mice in each, and they were treated with intraperitoneal injections of 0.02 mL/g saline, 0.10 and 0.20 mg/g CNL extract and 0.10 mg/g aminophenazone respectively. The intraperineal injection of 6 g/L glacial acetic acid was given after 15 minutes. The writhing times of mice within 15 minutes were observed and recorded in each group. ② Effects of CNL extract on the pain induced by hot pla in mice: Forty female mice were randomly divided into four groups with 10 mice in each, and they were treated with intraperineal injections of 0.02 mL/g saline, 0. 10 and 0.20 mg/g CNL extract and 0.10 mg/g morphine respectively. The pain responses were detected at 15, 30 and 60 minutes after administration. ③ The antagonistic effect of naloxone on morphine and CNL extract to the pain induced by hot plate in mice: Thirty female mice were randomly divided into three groups ith 10 mice in each group, and they were given intraperitoneal injections of 0.02 mL/g saline, naloxone 0.004 mg/g +morphine 0.01 mg/g and naloxone 0.004 mg/g+CNL extract 0.01 mg/g respectively. The pain responses were detected at 15, 30 and 60 minutes after administration respectively. ④ Effects of CNL extract on electrostimulation induced pain in mice: Forty mice were randomly divided into four groups with 10 mice in ach group, and they were administrated with intraperineal injections of 0.02 mL/g saline, 0.10 and 0.20 mg/g CNL extract and 1 g/L morphine respectively. Repeated electrostimulations were given at 20, 35, 50 and 70minutes after administration, and the pain responses were detected by means of electrostimulation.MAIN OUTCOME MEASURES: ① Writhing times; ② Time for the pain response induced by hot plate; ③ Analgesic rate induced by electrostimulation.RESULTS: Totally 150 healthy adult Kunming mice were used in the four independent experiments, and all were involved in the analysis of results. ①Writhing times in the mice: 0.10 and 0.20 mg/g CNL extracts and 0.10 mg/g aminophenazone had very significant analgesic effects on writhing induced byacetic acid in mice, and the writhing times after administration were all fewer than those in the saline group (20.2±10.8, 14.5±7.6, 7.6±4.5,50.6±15.5, P ＜ 0.01), and the analgesic effects of CNL extract were dosedependently. ② Time for the pain response induced by hot plate: 0.10 and 0.20 mg/g CNL extracts had significant analgesic effects on the pain in duced by hot plate, and the time for pain sensation at 15, 30 and 60 minutes after administration were all longer than those in the saline group (P ＜ 0.05 or P ＜ 0.01), and the analgesic effect was dose-dependently. The times for pain sensation at each time point after administration in the naloxone 0.004 mg/g+CNL extract 0.01 mg/g group were all longer than those in the saline group, but those were close between the naloxone 0.004 mg/g+morphine 0.01 mg/g group and the saline group. ③ Analgesic rate induced by electrostimulation in the mice: The analgesic rates at20, 35, 50 and 70minutes after administration in the CNL extract 0.10 and 0.20 mg/g groups were all higher than those in the saline group (P ＜ 0.01).CONCLUSION: Our data suggested that CNL extract has obvious analgesic effect, and the analgesic intensity is dose-dependently. Naloxone, an opiate receptor antagonist, can antagonize the analgesic effect of morphine,but cannot antagonize that of CNL extract on mice with pain induced by hot plate, which indicates that CNL extract exert its analgesic role not through binding with opiate receptor.
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