CAJ | 학술논문

为给板栗的分子遗传育种工作提供重要的分子数据参考,作者通过cDNA-AFLP的方法,以同株板栗幼年花序和叶片为实验材料,在转录水平上进行差示分析.结果显示:64对引物组合共产生了2131条PCR产物,对其中的110条克隆测序,发现有28条基因与已知功能基因相关,其中包括参与植物转运、代谢、能量产生相关的蛋白,从而证明这些基因在板栗生长发育中的重要作用.
위급판률적분자유전육충공작제공중요적분자수거삼고,작자통과cDNA-AFLP적방법,이동주판률유년화서화협편위실험재료,재전록수평상진행차시분석.결과현시:64대인물조합공산생료2131조PCR산물,대기중적110조극륭측서,발현유28조기인여이지공능기인상관,기중포괄삼여식물전운、대사、능양산생상관적단백,종이증명저사기인재판률생장발육중적중요작용.
In order to provide more references for chestnut molecular breeding, authors used the method of cDNA-AFLP to analyze gene expression profiles between inflorescences and leaves of chestnut. The results showed that: sixty-four combination of AFLP primers produced 2,131 fragments; twenty-eight genes showed significant homolog to known genes according to the sequencing of one hundred and ten genes, including transport facilitation, metabolism and energy-related proteins, which indicate the importance of them.