现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2009年
15期
2241-2242
,共2页
鼻咽肿瘤%比较基因组杂交
鼻嚥腫瘤%比較基因組雜交
비인종류%비교기인조잡교
Nasopharyngeal neoplasms%Comparative genomic hybridization
目的:探讨鼻咽癌三株不同细胞系CNE-1,HNE-2,5-8F的遗传变异特性.方法:采用比较基因组杂交技术,分析CNE-1,HNE-2,5-8F染色体的扩增与缺失.结果:共同的扩增区域为3q21-qter 5p13-pter,20q11-qter,12号染色体.共同的缺失区域为18号染色体,CNE-1与HNE-2相比,除T4p14-qter在CNE-1缺失,而HNE-2无明显变化外,其余的扩增与缺失区域均相同.而5-8F与CNE-1和HNE-2相比,则存在明显的不同.结论:鼻咽癌具有遗传变异,且不同细胞系之间染色体的扩增与缺失存在共同的区域,但亦有差异.
目的:探討鼻嚥癌三株不同細胞繫CNE-1,HNE-2,5-8F的遺傳變異特性.方法:採用比較基因組雜交技術,分析CNE-1,HNE-2,5-8F染色體的擴增與缺失.結果:共同的擴增區域為3q21-qter 5p13-pter,20q11-qter,12號染色體.共同的缺失區域為18號染色體,CNE-1與HNE-2相比,除T4p14-qter在CNE-1缺失,而HNE-2無明顯變化外,其餘的擴增與缺失區域均相同.而5-8F與CNE-1和HNE-2相比,則存在明顯的不同.結論:鼻嚥癌具有遺傳變異,且不同細胞繫之間染色體的擴增與缺失存在共同的區域,但亦有差異.
목적:탐토비인암삼주불동세포계CNE-1,HNE-2,5-8F적유전변이특성.방법:채용비교기인조잡교기술,분석CNE-1,HNE-2,5-8F염색체적확증여결실.결과:공동적확증구역위3q21-qter 5p13-pter,20q11-qter,12호염색체.공동적결실구역위18호염색체,CNE-1여HNE-2상비,제T4p14-qter재CNE-1결실,이HNE-2무명현변화외,기여적확증여결실구역균상동.이5-8F여CNE-1화HNE-2상비,칙존재명현적불동.결론:비인암구유유전변이,차불동세포계지간염색체적확증여결실존재공동적구역,단역유차이.
Objective:To observe the genetic alterations in different nasopharyngeal carc inoma(NPC) cell lines CNE-1, H NE-2 and 5-SF.Methods:The different NPC cell lines were detected by comparative genomic hybridization analysis in the gains and losses of chromosomes. Results:The common gains of chromosomes were 3q21-qter 5p13-pter,2q11-qter and chromosome 12.The common loss is chromosome 18.Compared with HNE-2,the only difference was the loss of 4p14-qter in CNE-1.Obvious difference existed in both the losses and gains of chromosomes between 5-8F and CNE-1.Conclusion:There are characteristic chromosome changes in nasopharyngeal carcinoma.Both sameness and difference exist in the chromosome changes of NPC cell lines.
