CAJ | 학술논문

目的:研究塔里木河天然胡杨林部分地区可培养细菌的生态分布.方法:通过塔里木河胡杨林采样,可培养菌分离及16S rDNA序列鉴定.结果:从3种不同样品(水样、土样和胡杨树杆分泌物)中分离筛选了22株细菌,其中17株菌为革兰氏阳性菌,5株为革兰氏阴性菌.根据生理生化特征与16S rDNA序列分析结果表明,其中15株菌属于芽孢杆菌属,4株属于不动杆菌属、假单胞菌属、动性球菌属和Agrococcus属各含有1个分离株.结论:塔里木河胡杨林可培养微生物中芽孢杆菌比较丰富,其中有3个可能的新种.
목적:연구탑리목하천연호양림부분지구가배양세균적생태분포.방법:통과탑리목하호양림채양,가배양균분리급16S rDNA서렬감정.결과:종3충불동양품(수양、토양화호양수간분비물)중분리사선료22주세균,기중17주균위혁란씨양성균,5주위혁란씨음성균.근거생리생화특정여16S rDNA서렬분석결과표명,기중15주균속우아포간균속,4주속우불동간균속、가단포균속、동성구균속화Agrococcus속각함유1개분리주.결론:탑리목하호양림가배양미생물중아포간균비교봉부,기중유3개가능적신충.
Objective: Investigate the bacteria community diversity in a small area of Natrural Populus euphractica Forest of Tarim River Valley, China. Method: Using phenotypic and biochemical characterization, and finally molecular identification by analysis of polymerase chain reaction(PCR)-amphfied 16S rRNA gene sequence.Result: 22 cultivable different bacteria had isolated from soil, waste water and secretion liquid the Poptdus euphractica stand by culturing with LB medium, respectively. Among them 17 strains were gram-positive, 5 strains were gram-negative. Based on analysis of 16S rRNA gene sequence showed that 15 strains found to be Bacilli, 4 strains were Acinetobacter, other than Ba-cilli and Acinetobacter there is one Pseudomonas strain(W1-1), one Planococcus strain (W2-3), and one Agrococcus strain (M5). Conclu-sion:There is a high diversity of Bacilli species in this small area, while some potential novel species were found belong to the Bacillus genus and ornithinibacillus.