CAJ | 학술논문

利用抑制性差减杂交文库筛选到的1个被乙烯诱导并与已知植物促丝裂原活化蛋白激酶（mitogen-activated protein kinase kinase,MAPKK;MKK）同源的cDNA片段,通过RACE技术得到该基因的全长cDNA序列。生物信息学分析表明,该基因属于植物MAPKK的A亚族,具有植物MAPKK的磷酸化一致序列S/T＊＊＊＊＊S/T。利用半定量RT-PCR检测该基因的表达量在乙烯处理后增强,推测其在乙烯诱导途径中起重要作用。
이용억제성차감잡교문고사선도적1개피을희유도병여이지식물촉사렬원활화단백격매（mitogen-activated protein kinase kinase,MAPKK;MKK）동원적cDNA편단,통과RACE기술득도해기인적전장cDNA서렬。생물신식학분석표명,해기인속우식물MAPKK적A아족,구유식물MAPKK적린산화일치서렬S/T＊＊＊＊＊S/T。이용반정량RT-PCR검측해기인적표체량재을희처리후증강,추측기재을희유도도경중기중요작용。
A cDNA fragments homologue of the known plant mitogen activated protein kinase（MAPKK,MKK,MEK） was selected by the suppression subtractive hybridization（SSH） library,and which can be induced by ethylene,and Rapid Amplification of cDNA Ends（RACE） were used to obtain the full length cDNA.Bioinformatic analysis results suggested that the gene belongs to a subfamily of plant MAPKK with a conserved region phosphorylation consensus sequence S/T＊＊＊＊＊S/T.Semi-quantitative RT-PCR results indicated that the expression of this gene increased after ethylene treatment,which suggested it may play an important role in the ethylene induction pathway.