国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2008年
3期
132-134
,共3页
张媛%童睿%郑秋月%谢明杰%曹际娟
張媛%童睿%鄭鞦月%謝明傑%曹際娟
장원%동예%정추월%사명걸%조제연
华支睾吸虫%PCR%实时荧光PCR
華支睪吸蟲%PCR%實時熒光PCR
화지고흡충%PCR%실시형광PCR
Clonorchis sinensis%PCR%Real-time PCR
目的 利用PCR技术建立快速、灵敏、特异的检测华支睾吸虫的方法,并比较PCR和实时荧光PCR检测华支睾吸虫的灵敏性和特异性,探讨其在华支睾吸虫检测中的应用价值.方法 根据华支睾吸虫的特异性基因序列,设计了PCR引物及实时荧光PCR的特异性引物和探针,分别进行灵敏性和特异性实验.结果 设计的引物能够扩增华支睾吸虫DNA,而且探针的特异性很高.实时荧光PCR对华支睾吸虫的检测阈值为3 fg/μl,灵敏度比PCR高两个数量级.结论 PCR和实时荧光PCR检测华支睾吸虫的灵敏性和特异性均很高,操作简便,为华支睾吸虫的检测提供了有效的技术手段和方法.
目的 利用PCR技術建立快速、靈敏、特異的檢測華支睪吸蟲的方法,併比較PCR和實時熒光PCR檢測華支睪吸蟲的靈敏性和特異性,探討其在華支睪吸蟲檢測中的應用價值.方法 根據華支睪吸蟲的特異性基因序列,設計瞭PCR引物及實時熒光PCR的特異性引物和探針,分彆進行靈敏性和特異性實驗.結果 設計的引物能夠擴增華支睪吸蟲DNA,而且探針的特異性很高.實時熒光PCR對華支睪吸蟲的檢測閾值為3 fg/μl,靈敏度比PCR高兩箇數量級.結論 PCR和實時熒光PCR檢測華支睪吸蟲的靈敏性和特異性均很高,操作簡便,為華支睪吸蟲的檢測提供瞭有效的技術手段和方法.
목적 이용PCR기술건립쾌속、령민、특이적검측화지고흡충적방법,병비교PCR화실시형광PCR검측화지고흡충적령민성화특이성,탐토기재화지고흡충검측중적응용개치.방법 근거화지고흡충적특이성기인서렬,설계료PCR인물급실시형광PCR적특이성인물화탐침,분별진행령민성화특이성실험.결과 설계적인물능구확증화지고흡충DNA,이차탐침적특이성흔고.실시형광PCR대화지고흡충적검측역치위3 fg/μl,령민도비PCR고량개수량급.결론 PCR화실시형광PCR검측화지고흡충적령민성화특이성균흔고,조작간편,위화지고흡충적검측제공료유효적기술수단화방법.
Objective To establish a quick, sensitive and specific method for detecting Clonorchis sinensis and evaluate the sensitivity and specificity of PCR and real-time PCR in detection of C. sinensis.Methods According to the specific sequence of C. sinensis genes, the primers of PCR and real-time PCR were designed separately and the fluorogenic probe was designed and synthesized. Results The designed primers could amplify the DNA of C.sinensis and the probes were of high specificity and stability.The detection thres hold by real-time PCR was 3 fg/μl,and the sensitivity of real-time PCR Was higher than PCR. Conclusion PCR and real-time PCR are both simple.rapid methods with hish sensitivity and specificity.Both of them provide an efficient way for detection of C. sinensis.
