中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2011年
7期
435-438
,共4页
郭建萍%孙晓麟%李英妮%吴新宇%何菁%李茹%安媛%赵静%潘思思%李春%栗占国
郭建萍%孫曉麟%李英妮%吳新宇%何菁%李茹%安媛%趙靜%潘思思%李春%慄佔國
곽건평%손효린%리영니%오신우%하정%리여%안원%조정%반사사%리춘%률점국
关节炎,类风湿%巨噬细胞%树突状细胞%巨噬细胞诱导性C型凝集素样受体
關節炎,類風濕%巨噬細胞%樹突狀細胞%巨噬細胞誘導性C型凝集素樣受體
관절염,류풍습%거서세포%수돌상세포%거서세포유도성C형응집소양수체
Arthritis,rheumatoid%Macrophages%Dendritic cells%Macrophage-inducible C-type lectin
目的 研究巨噬细胞诱导性C型凝集素样受体(MINCLE)在健康者、类风湿关节炎(RA)和骨关节炎患者外周血及关节滑液中的表达特征,探讨其与RA发病的相关性.方法 ①采用实时定量聚合酶链反应(PCR)方法在mRNA水平检测RA患者(253例)及健康人(71名)外周血单个核细胞(PBMC)中MINCLE的表达;②采用流式细胞术在蛋白水平检测RA患者(18例)、骨关节炎患者(5例)及健康人(12名)外周血及关节滑液巨噬细胞、髓样树突状细胞(mDC)和浆细胞样树突状细胞(pDC)中MINCLE的表达;采用Mann-Whitney U检验或配对t检验统计分析MINCLE在健康者、骨关节炎患者和RA患者外周血及关节滑液之间的表达差异.结果 ①在mRNA水平,外周血PBMC中RA患者的MINCLE表达达高于健康对照组[(1.65±0.36)和(0.37±0.06),U=6057,P=2.75×10-5];②在蛋白水平,健康人及骨关节炎患者外周血巨噬细胞、mDC及pDC中未见MINCLE的表达;在骨关节炎关节滑液中,MINCLE仅低水平表达于mDC[(7.5±2.9)%]中,而MINCLE在RA患者关节滑液mDC中呈高表达[(34.8±4.4)%,U=0,P=2.6×10-3];MINCLE在RA患者的滑液巨噬细胞及mDC的表达均明显高于其在相对应的外周血中的水平[巨噬细胞(2.01±0.53)%和(0.27±0.51)%,t=4.879.P=2.23×10-6;mDC为(34.8±4.4)%和(21.7±5.5)%,t=2.535,P=0.017].结论 MINCLE的表达特征具有RA组织特异性,MINCLE很可能为RA发病的一个重要标记物.
目的 研究巨噬細胞誘導性C型凝集素樣受體(MINCLE)在健康者、類風濕關節炎(RA)和骨關節炎患者外週血及關節滑液中的錶達特徵,探討其與RA髮病的相關性.方法 ①採用實時定量聚閤酶鏈反應(PCR)方法在mRNA水平檢測RA患者(253例)及健康人(71名)外週血單箇覈細胞(PBMC)中MINCLE的錶達;②採用流式細胞術在蛋白水平檢測RA患者(18例)、骨關節炎患者(5例)及健康人(12名)外週血及關節滑液巨噬細胞、髓樣樹突狀細胞(mDC)和漿細胞樣樹突狀細胞(pDC)中MINCLE的錶達;採用Mann-Whitney U檢驗或配對t檢驗統計分析MINCLE在健康者、骨關節炎患者和RA患者外週血及關節滑液之間的錶達差異.結果 ①在mRNA水平,外週血PBMC中RA患者的MINCLE錶達達高于健康對照組[(1.65±0.36)和(0.37±0.06),U=6057,P=2.75×10-5];②在蛋白水平,健康人及骨關節炎患者外週血巨噬細胞、mDC及pDC中未見MINCLE的錶達;在骨關節炎關節滑液中,MINCLE僅低水平錶達于mDC[(7.5±2.9)%]中,而MINCLE在RA患者關節滑液mDC中呈高錶達[(34.8±4.4)%,U=0,P=2.6×10-3];MINCLE在RA患者的滑液巨噬細胞及mDC的錶達均明顯高于其在相對應的外週血中的水平[巨噬細胞(2.01±0.53)%和(0.27±0.51)%,t=4.879.P=2.23×10-6;mDC為(34.8±4.4)%和(21.7±5.5)%,t=2.535,P=0.017].結論 MINCLE的錶達特徵具有RA組織特異性,MINCLE很可能為RA髮病的一箇重要標記物.
목적 연구거서세포유도성C형응집소양수체(MINCLE)재건강자、류풍습관절염(RA)화골관절염환자외주혈급관절활액중적표체특정,탐토기여RA발병적상관성.방법 ①채용실시정량취합매련반응(PCR)방법재mRNA수평검측RA환자(253례)급건강인(71명)외주혈단개핵세포(PBMC)중MINCLE적표체;②채용류식세포술재단백수평검측RA환자(18례)、골관절염환자(5례)급건강인(12명)외주혈급관절활액거서세포、수양수돌상세포(mDC)화장세포양수돌상세포(pDC)중MINCLE적표체;채용Mann-Whitney U검험혹배대t검험통계분석MINCLE재건강자、골관절염환자화RA환자외주혈급관절활액지간적표체차이.결과 ①재mRNA수평,외주혈PBMC중RA환자적MINCLE표체체고우건강대조조[(1.65±0.36)화(0.37±0.06),U=6057,P=2.75×10-5];②재단백수평,건강인급골관절염환자외주혈거서세포、mDC급pDC중미견MINCLE적표체;재골관절염관절활액중,MINCLE부저수평표체우mDC[(7.5±2.9)%]중,이MINCLE재RA환자관절활액mDC중정고표체[(34.8±4.4)%,U=0,P=2.6×10-3];MINCLE재RA환자적활액거서세포급mDC적표체균명현고우기재상대응적외주혈중적수평[거서세포(2.01±0.53)%화(0.27±0.51)%,t=4.879.P=2.23×10-6;mDC위(34.8±4.4)%화(21.7±5.5)%,t=2.535,P=0.017].결론 MINCLE적표체특정구유RA조직특이성,MINCLE흔가능위RA발병적일개중요표기물.
Objective To determine the expression pattern of macrophage-inducible c-type lectin (MINCLE)on Macrophage(Mφ),myeloid dendritic cell (mDC)and plasmacytoid DC(pDC)in peripheral blood (PB)and synovial fluid(SF)in patients with rheumatoid arthritis (RA).Methods For mRNA expression of MINCLE,253 RA patients and 71 healthy control subjects were enrolled.The mRNA level of MINCLE was determined by real-time PCR.For protein expression of MINCLE,18 patients with RA,5 patients with osteoarthritis(OA)and 12 healthy control subjects were enrolled.The expression of MINCLE on Mφ,mDC and pDC were detected by flow cytometry.The differences of MINCLE expressions in PB between RA patients,OA patients and healthy controls,or differences between PB and SF in RA patients were analyzed using Mann-Whitney U test or paired-samples t test.Results ①Compared to the healthy controls,RA patients showed elevated mRNA expression level of MINCLE in PBMCs[(1.65±0.36)vs (0.37±0.06),U=6057,P=2.75×10-5].②At protein level,MINCLE was hardly detected in Mφ,mDC and pDC in PB of OA patients and healthy controls.In SF,MINCLE was highiy expressed on mDC in RA patients,compared with that in OA patients[(34.8±4.4)%,U=0,P=2.6×10-3].In RA patients,the expression level of MINCLE was remarkably elevated in Mφ,mDC and pDC in SF compared with that in PB[Mφ(2.01±0.53)%vs(0.273±0.51)%,t=4.879,P=2.23×10-6;mDC(34.8±4.4)%vs(22.7±5.5)%t=2.535.P=0.017].Conclusion MINCLE is selectively expressed on Mφ.mDC and pDC in SF in RA patients.MINCLE may serve as a potential important marker,or even target,for RA and possibly even for inflammation in general.
