中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2011年
7期
510-515
,共6页
章沙沙%万小云%周彩云%吕卫国%谢幸
章沙沙%萬小雲%週綵雲%呂衛國%謝倖
장사사%만소운%주채운%려위국%사행
妊娠滋养细胞肿瘤%葡萄胎%免疫组织化学%磷蛋白磷酸酶%半胱氨酸内肽酶类
妊娠滋養細胞腫瘤%葡萄胎%免疫組織化學%燐蛋白燐痠酶%半胱氨痠內肽酶類
임신자양세포종류%포도태%면역조직화학%린단백린산매%반광안산내태매류
Gestational trophoblastic neoplasms%Hydatidiform mole%Immunohistochemistry%Phosphoprotein phosphatases%Cysteine endopeptidases
目的 榆测低分子质量多肽2(LMP2)和蛋白磷酸酶1A(PPM1A)在妊娠滋养细胞疾病组织中的表达,并探讨两者在预测葡萄胎恶变中的价值.方法 采用免疫组化EnVision二步法检测196例完全性葡萄胎(其中28例恶变)组织中LMP2和PPM1A蛋白的表达,选择妊娠滋养细胞肿瘤12例(侵蚀性葡萄胎7例、绒毛膜癌5例)和正常妊娠绒毛20例作为对照,回顾性分析其临床病理资料.结果 LMP2和PPM1A存细胞滋养细胞、合体滋养细胞和绒毛外滋养细胞中均有表达.LMP2在葡萄胎恶变者中的表达水平明显高于正常绒毛和葡萄胎良性转归者,分别为(6.79±2.38)、(3.10±1.65)、(5.26±2.63)分,分别比较,差异均有统计学意义(P均<0.01);而与妊娠滋养细胞肿瘤者[(6.42±2.68)分]比较,差异无统计学意义(P=0.113).PPM1A在正常绒毛、葡萄胎(良性转归、恶变)和妊娠滋养细胞肿瘤组织中的表达水平依次下调,分别为(6.30±2.98)、(4.93±2.50)、(4.43±2.04)、(3.33±2.06)分,分别比较,差异均有统汁学意义(P<0.01),且葡萄胎恶变者的表达低于良性转归者(P=0.001).LMP2表达与卵巢黄素化囊肿大小有关,PPM1A表达与子宫大小有关(P<0.05).LMP2与PPM1A的表达水平之间小存在相关性(P>0.05).结论 LMP2高表达和PPM1A低表达可能在滋养细胞的运动、侵袭及葡萄胎的恶性转化中发挥着重要作用.通过检测葡萄胎首次清官术组织中LMP2和PPM1A的表达,对判断葡萄胎的预后有一定的参考意义.
目的 榆測低分子質量多肽2(LMP2)和蛋白燐痠酶1A(PPM1A)在妊娠滋養細胞疾病組織中的錶達,併探討兩者在預測葡萄胎噁變中的價值.方法 採用免疫組化EnVision二步法檢測196例完全性葡萄胎(其中28例噁變)組織中LMP2和PPM1A蛋白的錶達,選擇妊娠滋養細胞腫瘤12例(侵蝕性葡萄胎7例、絨毛膜癌5例)和正常妊娠絨毛20例作為對照,迴顧性分析其臨床病理資料.結果 LMP2和PPM1A存細胞滋養細胞、閤體滋養細胞和絨毛外滋養細胞中均有錶達.LMP2在葡萄胎噁變者中的錶達水平明顯高于正常絨毛和葡萄胎良性轉歸者,分彆為(6.79±2.38)、(3.10±1.65)、(5.26±2.63)分,分彆比較,差異均有統計學意義(P均<0.01);而與妊娠滋養細胞腫瘤者[(6.42±2.68)分]比較,差異無統計學意義(P=0.113).PPM1A在正常絨毛、葡萄胎(良性轉歸、噁變)和妊娠滋養細胞腫瘤組織中的錶達水平依次下調,分彆為(6.30±2.98)、(4.93±2.50)、(4.43±2.04)、(3.33±2.06)分,分彆比較,差異均有統汁學意義(P<0.01),且葡萄胎噁變者的錶達低于良性轉歸者(P=0.001).LMP2錶達與卵巢黃素化囊腫大小有關,PPM1A錶達與子宮大小有關(P<0.05).LMP2與PPM1A的錶達水平之間小存在相關性(P>0.05).結論 LMP2高錶達和PPM1A低錶達可能在滋養細胞的運動、侵襲及葡萄胎的噁性轉化中髮揮著重要作用.通過檢測葡萄胎首次清官術組織中LMP2和PPM1A的錶達,對判斷葡萄胎的預後有一定的參攷意義.
목적 유측저분자질량다태2(LMP2)화단백린산매1A(PPM1A)재임신자양세포질병조직중적표체,병탐토량자재예측포도태악변중적개치.방법 채용면역조화EnVision이보법검측196례완전성포도태(기중28례악변)조직중LMP2화PPM1A단백적표체,선택임신자양세포종류12례(침식성포도태7례、융모막암5례)화정상임신융모20례작위대조,회고성분석기림상병리자료.결과 LMP2화PPM1A존세포자양세포、합체자양세포화융모외자양세포중균유표체.LMP2재포도태악변자중적표체수평명현고우정상융모화포도태량성전귀자,분별위(6.79±2.38)、(3.10±1.65)、(5.26±2.63)분,분별비교,차이균유통계학의의(P균<0.01);이여임신자양세포종류자[(6.42±2.68)분]비교,차이무통계학의의(P=0.113).PPM1A재정상융모、포도태(량성전귀、악변)화임신자양세포종류조직중적표체수평의차하조,분별위(6.30±2.98)、(4.93±2.50)、(4.43±2.04)、(3.33±2.06)분,분별비교,차이균유통즙학의의(P<0.01),차포도태악변자적표체저우량성전귀자(P=0.001).LMP2표체여란소황소화낭종대소유관,PPM1A표체여자궁대소유관(P<0.05).LMP2여PPM1A적표체수평지간소존재상관성(P>0.05).결론 LMP2고표체화PPM1A저표체가능재자양세포적운동、침습급포도태적악성전화중발휘착중요작용.통과검측포도태수차청관술조직중LMP2화PPM1A적표체,대판단포도태적예후유일정적삼고의의.
Objective To investigate the expression of low molecular mass polypeptide-2 (LMP2)and protein phosphatase 1A (PPM1A) in gestational trophoblastic disease and elucidate their predictive value in malignant transformation of hydatidiform mole. Methods The expressions of LMP2 and PPM1A protein in 196 complete hydatidiform moles (in which 28 cases with malignant transformation) , 7 invasive moles, 5 choriocarcinomas and 20 normal chorionic villus were detected with the method of En Vision immunohistochemistry. Their clinicopathologic data were retrospectively analyzed. Results LMP2 and PPM1A protein expressed in cytotrophocytes, syncytiotrophoblast and extravillous trophoblast. The level of LMP2 expression in deteriorative hydatidiform mole was significantly higher than that in non-deteriorative hydatidiform mole or normal chorionic villus (6. 79 ±2. 38, 5.26 ±2.63 and 3. 10 ±1.65, all P <0. 01),while there were no difference compared with gestational trophoblastic neoplasms (6. 42 ±2. 68, P=0. 113).The level of PPM1A expression was highest in normal chorionic villus, and decreased gradually in hydatidiform mole (non-deteriorative and deteriorative) and gestational trophoblastic neoplasms (6. 30 ±2. 98, 4. 93 ± 2. 50, 4. 43 ± 2. 04 and 3. 33 ± 2. 06, all P < 0. 01); the level of PPM1A expression in deteriorative hydatidiform mole was significantly lower than that in non-deteriorative hydatidiform mole (P=0.001). The expression of LMP2 protein was correlated to theca lutein ovarian cyst, the expression of PPM1A protein was related with uterine size (P < 0. 05) . While, there was no correlation between the expressions of the two proteins (P >0. 05). Conclusions High expression of LMP2 and low expression of PPM1A might play an important role in the motility and invasiveness of trophohlast cells and malignant transformation of hydatidiform mole. Testing the expression of LMP2 and PPM1A in hydatidiform mole tissues of initial uterine evacuation might be have some reference significance in judging outcomes of hydatidiform mole.
