CAJ | 학술논문

目的观察含母牛分枝杆菌菌苗(微卡)凝胶注入肺部后的吸收和安全性,并对注入的适宜剂量和不良反应进行分析,为可用于肺结核局部免疫治疗提供依据.方法 50只日本长耳兔随机分成5组,每组10只,气管插管至末梢支气管,注入相关制剂.第1组为对照组,注人生理盐水3 ml;第2组为单纯凝胶组,注入卡波姆凝胶3 ml;第3、4、5组为微卡凝胶组,分别注入含3.75、7.50、9.37 mg/L母牛分枝杆菌菌苗的卡波姆凝胶3 ml.持续观察4周,期间检测体温、体质量、血常规,实验结束时处死动物,解剖相关器官及进行病理学观察.结果各组间体温、体质量、血常规的比较差异均无统计学意义.第2、3组与第1组相比,肺大体及光镜病理观察均未产生明显变化.第4、5组与第1组相比,细支气管未发生明显变化,肺泡结构基本保持完整,间隔轻度增宽,间隔内可见淋巴细胞浸润.结论含母牛分枝杆菌菌苗凝胶肺内应用具有较好的安全性.
목적 관찰함모우분지간균균묘(미잡)응효주입폐부후적흡수화안전성,병대주입적괄의제량화불량반응진행분석,위가용우폐결핵국부면역치료제공의거.방법 50지일본장이토수궤분성5조,매조10지,기관삽관지말소지기관,주입상관제제.제1조위대조조,주인생리염수3 ml;제2조위단순응효조,주입잡파모응효3 ml;제3、4、5조위미잡응효조,분별주입함3.75、7.50、9.37 mg/L모우분지간균균묘적잡파모응효3 ml.지속관찰4주,기간검측체온、체질량、혈상규,실험결속시처사동물,해부상관기관급진행병이학관찰.결과 각조간체온、체질량、혈상규적비교차이균무통계학의의.제2、3조여제1조상비,폐대체급광경병리관찰균미산생명현변화.제4、5조여제1조상비,세지기관미발생명현변화,폐포결구기본보지완정,간격경도증관,간격내가견림파세포침윤.결론 함모우분지간균균묘응효폐내응용구유교호적안전성.
Objective To provide basis for the local immune therapy of tuberculosis by observing the absorption and security of Mycobacterium vaccae (micro card) gel after administered into lung and analysing the optimum dose and side effects. Methods Fifty Japanese long-eared rabbits were randomly divided into five groups. Group 1 was injected with 3 ml normal sodium. Group 2 was injected with 3 ml carbomer gel. Group 3, 4, 5 were injected with 3 ml carbomer gel with 3.75, 7.50, 9.37 mg/L Mycobacterium vaccae. The temperature, weight, blood were tested durirg four weeks. The animals were sacrificed at the end and tissue pathology was observed. Results There was no statistical significance on temperature, weight, blood among all groups. Compared with group 1, the changes of pathology in lung of group 2 and group 3 were not obvious. There were no obvious changes on the bronchiole between group 4,5 and group 1, alveolar structure remained intact, interval mildly widened,and there was lymphocytic infiltration in interval. Conclusions Gel with Mycobacterium vaccae administered in lung has better security.