国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2011年
5期
550-554
,共5页
乳腺癌%密集化疗%术后复发高危因素%调节性T细胞%AKT蛋白
乳腺癌%密集化療%術後複髮高危因素%調節性T細胞%AKT蛋白
유선암%밀집화료%술후복발고위인소%조절성T세포%AKT단백
Breast cancer%Intensive chemotherapy%High risks for postoperative recurrence%Regulatory T cells%Protein AKT
目的 探讨密集化疗方案对乳腺癌患者调节性T细胞及AKT蛋白含量的影响.方法 收集具有术后复发高危因素的乳腺癌患者36例并随机分为观察组及对照组各18例,前者给予表阿霉素(EPI)联合环磷酰胺(CTX)并序贯紫杉醇(PTX)的2周化疗方案,后者给予EPI联合PTX的3周化疗方案;于化疗前及化疗结束后1周分别采集患者的外周血,采用流式细胞术检测CD4+CD25+调节性T细胞(Treg)的数量;使用免疫组化SP法对病灶活检进行AKT蛋白的免疫组化检测,并采用图像分析软件对AKT蛋白的含量进行定量分析.结果 治疗前两组患者的Treg数量及AKT蛋白表达比较,差异均无显著性(P＞0.05);治疗后观察组患者Treg下降为(9.32±2.47)%,明显低于对照组的(12.96±3.65)%,差异有显著性(P=0.021);治疗后观察组AKT抗原表达密度比值下降为(0.371±0.096),对照组为(0.512±0.108),组间差异具有显著性(P=0.016);相关性分析显示,Treg与AKT蛋白含量呈显著正相关性(r=0.786,P=0.013).结论 密集化疗方案较常规方案更加有效降低具有术后复发高危因素的乳腺癌患者的Treg及AKT蛋白.
目的 探討密集化療方案對乳腺癌患者調節性T細胞及AKT蛋白含量的影響.方法 收集具有術後複髮高危因素的乳腺癌患者36例併隨機分為觀察組及對照組各18例,前者給予錶阿黴素(EPI)聯閤環燐酰胺(CTX)併序貫紫杉醇(PTX)的2週化療方案,後者給予EPI聯閤PTX的3週化療方案;于化療前及化療結束後1週分彆採集患者的外週血,採用流式細胞術檢測CD4+CD25+調節性T細胞(Treg)的數量;使用免疫組化SP法對病竈活檢進行AKT蛋白的免疫組化檢測,併採用圖像分析軟件對AKT蛋白的含量進行定量分析.結果 治療前兩組患者的Treg數量及AKT蛋白錶達比較,差異均無顯著性(P＞0.05);治療後觀察組患者Treg下降為(9.32±2.47)%,明顯低于對照組的(12.96±3.65)%,差異有顯著性(P=0.021);治療後觀察組AKT抗原錶達密度比值下降為(0.371±0.096),對照組為(0.512±0.108),組間差異具有顯著性(P=0.016);相關性分析顯示,Treg與AKT蛋白含量呈顯著正相關性(r=0.786,P=0.013).結論 密集化療方案較常規方案更加有效降低具有術後複髮高危因素的乳腺癌患者的Treg及AKT蛋白.
목적 탐토밀집화료방안대유선암환자조절성T세포급AKT단백함량적영향.방법 수집구유술후복발고위인소적유선암환자36례병수궤분위관찰조급대조조각18례,전자급여표아매소(EPI)연합배린선알(CTX)병서관자삼순(PTX)적2주화료방안,후자급여EPI연합PTX적3주화료방안;우화료전급화료결속후1주분별채집환자적외주혈,채용류식세포술검측CD4+CD25+조절성T세포(Treg)적수량;사용면역조화SP법대병조활검진행AKT단백적면역조화검측,병채용도상분석연건대AKT단백적함량진행정량분석.결과 치료전량조환자적Treg수량급AKT단백표체비교,차이균무현저성(P＞0.05);치료후관찰조환자Treg하강위(9.32±2.47)%,명현저우대조조적(12.96±3.65)%,차이유현저성(P=0.021);치료후관찰조AKT항원표체밀도비치하강위(0.371±0.096),대조조위(0.512±0.108),조간차이구유현저성(P=0.016);상관성분석현시,Treg여AKT단백함량정현저정상관성(r=0.786,P=0.013).결론 밀집화료방안교상규방안경가유효강저구유술후복발고위인소적유선암환자적Treg급AKT단백.
Objective To explore the effect of intensive chemotherapy on levels of regulatory T cells (Treg) and protein AKT in patients with breast cancer. Methods 36 patients with high risk factors for postoperative recurrence were randomly assigned to receive EPI combined with CTX and PTX for two weeks (study group, 18 patients) or EPI plus PTX for 3 weeks. The amounts of Treg, CD4 and CD25 were detected by flow cytometry and AKT level was determined by immunohistochemistry before and one week after chemotherapy. Results The baseline Treg amount and AKT level did not differ significantly between the two groups (P＞0.05). After chemotherapy, the rate of Treg was significantly lower in the study group than in the control group [(9.32 ± 2.47)% vs. (2.96 ± 3.65)%, P= 0.021]; the expression ratio of AKT antigen was significantly lower in the study group than in the control group [(0.371±0.096)vs.(0.512± 0. 108), P= 0.016]. Treg level was positively related with A KT level (r = 0.786, P= 0.013). Conclusions Intensive chemotherapy is more effective than regular chemotherapy in decreasing the expressions of regulatory T cells and protein AKT in patients with breast cancer.
