中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
10期
829-833
,共5页
基因表达%脓毒症%脾%乌司他丁
基因錶達%膿毒癥%脾%烏司他丁
기인표체%농독증%비%오사타정
Gene expression%Sepsis%Spleen%Ulinstatin
目的 应用基因芯片技术观察乌司他丁(Ulinastatin UTI)预处理脓毒症大鼠脾组织基因表达谱的变化.方法 将45只雄性Wistar大鼠按随机数字表法平均分为假手术组、脓毒症组和UTI组.采用盲肠结扎穿孔术复制脓毒症大鼠模型.UTI组于制模前1 h肌肉注射UTI10万单位/kg；脓毒症组及假手术组肌注平衡液5 ml/kg.采用RatRef-12大鼠表达谱基因芯片进行检测,用计算机软件筛选并分析比较脓毒症组、UTI组与假手术组大鼠脾组织基因表达的变化,并分析脓毒症组和UTI组表达基因的差异.结果 在22 523个基因中,与假手术组比较,脓毒症组大鼠脾组织差异表达基因共205个,占基因芯片总点数的0.910％；其中表达上调者98个,已知功能基因48个,仅在脓毒症组出现32个；表达下调者107个,已知功能基因64个,仅在脓毒症组出现34个.与假手术组比较,UTI组大鼠脾组织差异表达基因共197个,占基因芯片总点数的0.875％.其中表达上调者114个,已知功能基因35个,仅在UTI组出现19个；表达下调者83个,已知功能基因49个,仅在UTI组出现19个.结论 UTI预处理可在一定程度上纠正部分脓毒症大鼠过度炎症反应及免疫抑制所致脾组织基因表达异常,在基因水平上对脾组织有保护作用.
目的 應用基因芯片技術觀察烏司他丁(Ulinastatin UTI)預處理膿毒癥大鼠脾組織基因錶達譜的變化.方法 將45隻雄性Wistar大鼠按隨機數字錶法平均分為假手術組、膿毒癥組和UTI組.採用盲腸結扎穿孔術複製膿毒癥大鼠模型.UTI組于製模前1 h肌肉註射UTI10萬單位/kg；膿毒癥組及假手術組肌註平衡液5 ml/kg.採用RatRef-12大鼠錶達譜基因芯片進行檢測,用計算機軟件篩選併分析比較膿毒癥組、UTI組與假手術組大鼠脾組織基因錶達的變化,併分析膿毒癥組和UTI組錶達基因的差異.結果 在22 523箇基因中,與假手術組比較,膿毒癥組大鼠脾組織差異錶達基因共205箇,佔基因芯片總點數的0.910％；其中錶達上調者98箇,已知功能基因48箇,僅在膿毒癥組齣現32箇；錶達下調者107箇,已知功能基因64箇,僅在膿毒癥組齣現34箇.與假手術組比較,UTI組大鼠脾組織差異錶達基因共197箇,佔基因芯片總點數的0.875％.其中錶達上調者114箇,已知功能基因35箇,僅在UTI組齣現19箇；錶達下調者83箇,已知功能基因49箇,僅在UTI組齣現19箇.結論 UTI預處理可在一定程度上糾正部分膿毒癥大鼠過度炎癥反應及免疫抑製所緻脾組織基因錶達異常,在基因水平上對脾組織有保護作用.
목적 응용기인심편기술관찰오사타정(Ulinastatin UTI)예처리농독증대서비조직기인표체보적변화.방법 장45지웅성Wistar대서안수궤수자표법평균분위가수술조、농독증조화UTI조.채용맹장결찰천공술복제농독증대서모형.UTI조우제모전1 h기육주사UTI10만단위/kg；농독증조급가수술조기주평형액5 ml/kg.채용RatRef-12대서표체보기인심편진행검측,용계산궤연건사선병분석비교농독증조、UTI조여가수술조대서비조직기인표체적변화,병분석농독증조화UTI조표체기인적차이.결과 재22 523개기인중,여가수술조비교,농독증조대서비조직차이표체기인공205개,점기인심편총점수적0.910％；기중표체상조자98개,이지공능기인48개,부재농독증조출현32개；표체하조자107개,이지공능기인64개,부재농독증조출현34개.여가수술조비교,UTI조대서비조직차이표체기인공197개,점기인심편총점수적0.875％.기중표체상조자114개,이지공능기인35개,부재UTI조출현19개；표체하조자83개,이지공능기인49개,부재UTI조출현19개.결론 UTI예처리가재일정정도상규정부분농독증대서과도염증반응급면역억제소치비조직기인표체이상,재기인수평상대비조직유보호작용.
Objective To explore the effect of UTI (Ulinastatin) preconditioning on gene expression profiles of spleen tissue in septic rats by DNA microarray technology. Methods Forty-five male Wistar rats were equally divided into sham group,sepsis group and UTI group by means of random number table.In UTI group the rats were treated with intramuscular injection of UTI( 105 U/kg) one hour before cecal ligation and puncture.In sepsis group and sham group intramuscular balanced solution (5 ml/kg) was given.Cecal ligation and puncture was used to reproduce septic rat model. Gene expression profile was studied by using RatRef-12 Rat gene expression profile microarray to detect the changes in gene expression pattern of rat spleen tissue after cecal ligation and puncture.Then using related computer software was used to screen and analyze the relationship between the Sepsis/UTI group and sham group. Results In 22 523 genes,205 differential genes were found between sepsis group and sham group,accounting for 0.910％.Among them 98 genes were up-regulated,with 48 known functional genes and 32 genes only showed in this group;107 genes were down-regulated,with 64 known functional genes and 34 genes only showed in it.197 differential genes were found in UTI group and sham group,accounting for 0.875％.Among them 114 genes were up-regulated,with 35 known functional genes and 19 genes only showed in this group; 83 genes were down-regulated,with 49 known functional genes and 19 genes only showed in it. Conclusions Abnormal expression of genes in the spleen tissue of rats with sepsis owing to excessive inflammation and immune suppression were partly relieved by UTI preconditioning.UTI pretects spleen at genetic level.
