CAJ | 학술논문

目的对分离的262株嗜肺军团菌进行脉冲场凝胶电泳(PFGE)分析,初步建立中国嗜肺军团菌的PFGE分型数据库.方法采用PFGE技术,对2004-2009年中国11个省(市)分离的嗜肺军团菌用AscⅠ酶切,BioNumerics软件分析PFGE图谱,并建立分子分型数据库.结果 262株嗜肺军团菌的PFGE图谱共分为108种不同的PFGE带型,相似性系数在16%～100%之间,通过聚类分析,可以分为差异明显的不同簇.不同省份、年份和血清型的菌株之间有交叉带型.结论中国环境分离的嗜肺军团菌菌株基因组变异较大,同时也具有克隆化特征,且可能有多个克隆系存在.
목적 대분리적262주기폐군단균진행맥충장응효전영(PFGE)분석,초보건립중국기폐군단균적PFGE분형수거고.방법 채용PFGE기술,대2004-2009년중국11개성(시)분리적기폐군단균용AscⅠ매절,BioNumerics연건분석PFGE도보,병건립분자분형수거고.결과 262주기폐군단균적PFGE도보공분위108충불동적PFGE대형,상사성계수재16%～100%지간,통과취류분석,가이분위차이명현적불동족.불동성빈、년빈화혈청형적균주지간유교차대형.결론 중국배경분리적기폐군단균균주기인조변이교대,동시야구유극륭화특정,차가능유다개극륭계존재.
Objective To analyze the molecular types of Legionella (L.) pneumophila strains isolated in China,and to develop the PulseNet-China Database of L.pneumophila.Methods Pulsed field gel electrophoresis (PFGE) was used to analyze 262 L.pneumophila strains collected from 11 provinces between 2004 and 2009 in China.Different kinds of genomic DNA in different L.pneumophila strains were isolated and separated after digesting with Asc Ⅰ.BioNumerics software was used to analysis the PFGE fingerprints.Results L.pneumophila strains isolated in China were quite different regarding their PFGE patterns.There were 108 PFGE types among the 262 strains tested in this study.The similarity value of these strains was in the range of 16%-100% and the same types were discovered in different provinces and years.Conclusion L.pneumophila strains isolated in China were with high genetic variations.There might be different clones existed in China.The development of PulseNet China Database was thus of great significance in monitoring the L.pneumophila strains in the future.