中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
2期
286-287
,共2页
己烯雌酚%表皮生长因子%睾丸
己烯雌酚%錶皮生長因子%睪汍
기희자분%표피생장인자%고환
Diethylstilbestrol%Epidermal growth factor%Testis
目的 观察不同剂量己烯雌酚(DES)对新生小鼠睾丸组织中表皮生长因子(EGF)及其受体(EGFR)的影响,探讨DES对睾丸发育影响的机制.方法 建立小鼠DES模型.将72只怀孕的雌性昆明小鼠随机分成3组:正常组、对照组及实验组1～4(DES 10、25、50、100 μg/kg).采用免疫组织化学方法检测各组新生小鼠睾丸组织中EGF、EGFR的表达.结果 EGF和EGFR主要表达在新生小鼠睾丸的间质细胞.实验组1～4中EGF阳性细胞的累积吸光度值(IA)分别为75.43±1. 42、52.22±5.67、13.75±3.14、6.38±3.20,显著低于正常组及对照组中阳性细胞的IA值433.88±11.64、23.44±4.70;实验组EGFR阳性细胞的IA值分别为198.16±34.35、138.00±12.04、46.03±6.74、27.22±5.52,显著低于正常组及对照组中阳性细胞的IA值804.74±22.52、800.03±21.96.两者在正常、不同剂量DES实验组的两两比较差异有统计学意义(P＜0.05).随DES剂量增加,EGF和EGFR表达减弱,各组间差异有统计学意义(P＜0.05).EGF与EGFR呈强正线性相关(r=0.750,P＜0.01).结论 不同剂量DES对新生小鼠睾丸组织中EGF和EGFR表达强度均有影响,可能是影响睾丸发育机制之一.
目的 觀察不同劑量己烯雌酚(DES)對新生小鼠睪汍組織中錶皮生長因子(EGF)及其受體(EGFR)的影響,探討DES對睪汍髮育影響的機製.方法 建立小鼠DES模型.將72隻懷孕的雌性昆明小鼠隨機分成3組:正常組、對照組及實驗組1～4(DES 10、25、50、100 μg/kg).採用免疫組織化學方法檢測各組新生小鼠睪汍組織中EGF、EGFR的錶達.結果 EGF和EGFR主要錶達在新生小鼠睪汍的間質細胞.實驗組1～4中EGF暘性細胞的纍積吸光度值(IA)分彆為75.43±1. 42、52.22±5.67、13.75±3.14、6.38±3.20,顯著低于正常組及對照組中暘性細胞的IA值433.88±11.64、23.44±4.70;實驗組EGFR暘性細胞的IA值分彆為198.16±34.35、138.00±12.04、46.03±6.74、27.22±5.52,顯著低于正常組及對照組中暘性細胞的IA值804.74±22.52、800.03±21.96.兩者在正常、不同劑量DES實驗組的兩兩比較差異有統計學意義(P＜0.05).隨DES劑量增加,EGF和EGFR錶達減弱,各組間差異有統計學意義(P＜0.05).EGF與EGFR呈彊正線性相關(r=0.750,P＜0.01).結論 不同劑量DES對新生小鼠睪汍組織中EGF和EGFR錶達彊度均有影響,可能是影響睪汍髮育機製之一.
목적 관찰불동제량기희자분(DES)대신생소서고환조직중표피생장인자(EGF)급기수체(EGFR)적영향,탐토DES대고환발육영향적궤제.방법 건립소서DES모형.장72지부잉적자성곤명소서수궤분성3조:정상조、대조조급실험조1～4(DES 10、25、50、100 μg/kg).채용면역조직화학방법검측각조신생소서고환조직중EGF、EGFR적표체.결과 EGF화EGFR주요표체재신생소서고환적간질세포.실험조1～4중EGF양성세포적루적흡광도치(IA)분별위75.43±1. 42、52.22±5.67、13.75±3.14、6.38±3.20,현저저우정상조급대조조중양성세포적IA치433.88±11.64、23.44±4.70;실험조EGFR양성세포적IA치분별위198.16±34.35、138.00±12.04、46.03±6.74、27.22±5.52,현저저우정상조급대조조중양성세포적IA치804.74±22.52、800.03±21.96.량자재정상、불동제량DES실험조적량량비교차이유통계학의의(P＜0.05).수DES제량증가,EGF화EGFR표체감약,각조간차이유통계학의의(P＜0.05).EGF여EGFR정강정선성상관(r=0.750,P＜0.01).결론 불동제량DES대신생소서고환조직중EGF화EGFR표체강도균유영향,가능시영향고환발육궤제지일.
Objective To investigate the effects of prenatal exposure to diethylstilbestrol (DES)with different dosages on epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in offspring mice testis, and the possible mechanism. Methods DES model was induced in mice by DES.Female Kungmiag mice were randomly divided into normal group, control group and experimental groups 1-4 ( DES 10, 25, 50, 100 μg/kg). Immunohistochemistry was applied to detect the expression of EGF and EGFR in the testicular tissue in each group. Results EGF and EGFR were expressed mainly in sffspring mice testis Leydig cells. The cumulative absorbance ( IA ) values of EGF positive cells in experimental groups 1-4 were 75. 43 ± 1.42, 52. 22 ± 5.67, 13.75 ± 3. 14, and 6. 38 ± 3.20 respectively, which were significantly lower than in normal and control groups (433.88 ± 11.64,423.44 ±4. 70 respectively).The IA values of EGFR positive cells in experimental groups 1-4 were 198. 16 ± 34. 35, 138.00 ± 12.04,46.03 ± 6. 74, 27.22 ± 5.52 respectively, which were significantly lower than in normal and control groups (804. 74 ± 22. 52,800. 03 ± 21.96 respectively). The expression levels of EGF and EGFR could be detected in each subgroup and statistically significant differences existed in the expression of EGF and EGFR between any two groups ( P ＜ 0. 05 ). With the increase of DES dosage, the expression of EGF and EGFR was decreased, with the difference being significant amond the groups ( P ＜ 0. 05 ). Conclusion DES can influence the expression of EGF and EGFR in mice testis, which might be one of possible mechanisms effecting the development of testis.
